Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Malarz, Janusz; Michalska, Klaudia; Yudina, Yulia V.; Stojakowska, Anna

doi:10.3390/plants11151950

Cited by 17 publications

(8 citation statements)

References 216 publications

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“…Further studies on the elicitation of hairy root cultures [172][173][174][175][176] highlighted the potential to produce higher amounts of secondary metabolites. Hashemi and Naghavi [172] demonstrated elicitation in the hairy root culture of Papverorientale with methyl jasmonate and salicylic acid, which resulted in the regulation of the expression of genes in the morphine pathway; moreover, the elicitation of methyl jasmonate (MJ) improved the synthesis of thebaine (3.08 mg/g), morphine (5.38 mg/g) and codeine (2.57 mg/g).…”

Section: Elicitation Of In Vitro Productsmentioning

confidence: 99%

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

et al. 2022

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Medicinal plants are rich sources of bioactive compounds widely used as medicaments, food additives, perfumes, and agrochemicals. These secondary compounds are produced under stress conditions to carry out physiological tasks in plants. Secondary metabolites have a complex chemical structure with pharmacological properties. The widespread use of these metabolites in a lot of industrial sectors has raised the need to increase the production of secondary metabolites. Biotechnological methods of cell culture allow the conservation of plants, as well as the improvement of metabolite biosynthesis and the possibility to modify the synthesis pathways. The objective of this review is to outline the applications of different in vitro culture systems with previously reported relevant examples for the optimal production of plant-derived secondary metabolites.

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Section: Elicitation Of In Vitro Productsmentioning

confidence: 99%

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

et al. 2022

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“…Finally, glyceollins I, II, and III are produced through the condensation of (-)-glycinol and prenyl diphosphate. The addition of prenyl diphosphate in the position 4 of (-)-glycinol is needed to produce glyceollin I, while the addition in position 2 for glyceollins II and III [ 45 , 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1

Lin

Monsalvo

et al. 2023

Plants

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Glyceollins, isoflavonoid-derived antimicrobial metabolites, are the major phytoalexins in soybean (Glycine max). They play essential roles in providing resistance to the soil-borne pathogen Phytophthora sojae and have unconventional anticancer and neuroprotective activities that render them desirable for pharmaceutical development. Our previous studies revealed that the transcription factors GmMYB29A2 and GmNAC42-1 have essential roles in activating glyceollin biosynthesis, yet each cannot activate the transcription of all biosynthesis genes in the absence of a pathogen elicitor treatment. Here, we report that co-overexpressing both transcription factors is also insufficient to activate glyceollin biosynthesis. To understand this insufficiency, we compared the transcriptome profiles of hairy roots overexpressing each transcription factor with glyceollin-synthesizing roots treated with wall glucan elicitor (WGE) from P. sojae. GmMYB29A2 upregulated most of the WGE-regulated genes that encode enzymatic steps spanning from primary metabolism to the last step of glyceollin biosynthesis. By contrast, GmNAC42-1 upregulated glyceollin biosynthesis genes only when overexpressed in the presence of WGE treatment. This is consistent with our recent discovery that, in the absence of WGE, GmNAC42-1 is bound by GmJAZ1 proteins that inhibit its transactivation activity. WGE, and not GmMYB29A2 or GmNAC42-1, upregulated the heat shock family gene GmHSF6-1, the homolog of Arabidopsis HSFB2a that directly activated the transcription of several glyceollin biosynthesis genes. Our results provide important insights into what biosynthesis genes will need to be upregulated to activate the entire glyceollin biosynthetic pathway.

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“…In our previous paper [ 26 ], we summarized the attempts to produce flavonoids, stilbenoids, and hydrolysable tannins in HRs. However, plant polyphenolics are a much larger group of natural products and encompass further subclasses of plant metabolites.…”

Section: Introductionmentioning

confidence: 99%

Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates

Malarz

Yudina

Stojakowska

2023

IJMS

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Plant-derived antioxidants are intrinsic components of human diet and factors implicated in tolerance mechanisms against environmental stresses in both plants and humans. They are being used as food preservatives and additives or ingredients of cosmetics. For nearly forty years, Rhizobium rhizogenes-transformed roots (hairy roots) have been studied in respect to their usability as producers of plant specialized metabolites of different, primarily medical applications. Moreover, the hairy root cultures have proven their value as a tool in crop plant improvement and in plant secondary metabolism investigations. Though cultivated plants remain a major source of plant polyphenolics of economic importance, the decline in biodiversity caused by climate changes and overexploitation of natural resources may increase the interest in hairy roots as a productive and renewable source of biologically active compounds. The present review examines hairy roots as efficient producers of simple phenolics, phenylethanoids, and hydroxycinnamates of plant origin and summarizes efforts to maximize the product yield. Attempts to use Rhizobium rhizogenes-mediated genetic transformation for inducing enhanced production of the plant phenolics/polyphenolics in crop plants are also mentioned.

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Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Cited by 17 publications

References 216 publications

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1

Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates

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