Correlation of 16s rRNA with serum levels of the cytokines, TNF-α and IL-1β, in subjects with a positive Helicobacter pylori Stool Antigen test (HPSA)

Moradipour, Ayat; Khosravi, Afra; Mehrabi, Mohammad Reza; Faryadian, Sara

doi:10.18869/acadpub.jbrms.3.2.35

Cited by 3 publications

(6 citation statements)

References 17 publications

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“…Similarly, the H. pylori infection also increases the expression of IL-1β, which results in the decreased secretion of gastric acid. This phenomenon agrees with the report that given the increased production of IL-1β mouse would develop gastric cancer 31 , 37 . Moreover, a high gene expression level of the H. pylori 16s rRNA in feces is positively correlated with the TNF-α activity.…”

Section: Discussionsupporting

confidence: 93%

Fucoidan from Sargassum hemiphyllum inhibits infection and inflammation of Helicobacter pylori

Chen

et al. 2022

Sci Rep

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Having infected by Helicobacter pylori, the infection often leads to gastritis, gastric ulcer, or even gastric cancer. The disease is typically treated with antibiotics as they used to effectively inhibit or kill H. pylori, thus reducing the incidence of gastric adenoma and cancer to significant extent. H. pylori, however, has developed drug resistance to many clinically used antibiotics over the years, highlighting the crisis of antibiotic failure during the H. pylori treatment. We report here that the fucoidan from Sargassum hemiphyllum can significantly reduce the infection of H. pylori without developing to drug resistance. Fucoidan appears to be a strong anti-inflammation agent as manifested by the RAW264.7 cell model examination. Fucoidan can prohibit H. pylori adhesion to host cells, thereby reducing the infection rate by 60%, especially in post treatment in the AGS cell model assay. Mechanistically, fucoidan intervenes the adhesion of BabA and AlpA of H. pylori significantly lowering the total count of H. pylori and the level of IL-6 and TNF-α in vivo. These results all converge on the same fact that fucoidan is an effective agent in a position to protect the stomach from the H. pylori infection by reducing both the total count and induced inflammation.

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Section: Discussionsupporting

confidence: 93%

Fucoidan from Sargassum hemiphyllum inhibits infection and inflammation of Helicobacter pylori

Chen

et al. 2022

Sci Rep

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“…Among them, IL-6 activates the STAT3 pathway, which is correlated to H. pylori-associated gastritis and gastric cancer 23 . Similarly, H. pylori infection increases expression of IL-1β, which results in decreased secretion of gastric acid agreeing with the report that mouse developed gastric cancer given increased production of IL-1β [24]. Moreover, a higher H. pylori 16s rRNA gene expression in feces was detected, which is positively correlated with TNF-α activity.…”

Section: Discussionsupporting

confidence: 89%

“…Moreover, a higher H. pylori 16s rRNA gene expression in feces was detected, which is positively correlated with TNF-α activity. High levels of TNF-α normally come with chronic in ammation 24 . While fucoidan did not signi cantly reduce the level of IL-1β, it signi cantly decreased the level of IL-6 and TNF-α in mice.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan from Sargassum Hemiphyllum Inhibits Infection and Inflammation of Helicobacter Pylori

Chen

et al. 2021

Preprint

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When infected by Helicobacter pylori, it often causes gastritis, gastric ulcer, or gastric cancer. Antibiotics are used to treat H. pylori infection, as they inhibit or kill H. pylori often ex-tending to reduce the incidences of gastric adenoma and cancer. However, H. pylori has developed drug resistance to many clinically used antibiotics over the years, thereby providing no warranty of successful treatment whenever H. pylori infection befalls. We report here that fucoidan from Sargassumhemiphyllum can effectively reduce infection of H. pylori without development of drug resistance. Fucoidan demonstrated a strong anti-inflammation activity in RAW264.7 cell model. Using AGS cell model, fucoidan decreased H. pylori adhesion to host cells and thus reduced its infection rate, especially in post-treatment where the infection rate was reduced to 40%. Mechanistically, fucoidan intervenes the proper functions of adhesion molecules BabA and AlpA of H. pylori. Moreover, fucoidan is able to significantly lower the total count of H. pylori and the levels of IL-6 and TNF-α in vivo. Added together, these convergent results suggest that fucoidan is an effective agent in a position to protect stomach from H. pylori infection by reducing its total count and induced inflammation.

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“…According to the World Health Organization, approximately 50% of adults in developed countries and about 90% of adults in developing countries are suffering from H. pylori infection [2]. In developing countries, 70%-80% of children under the age of 15 years are infected with this bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…Helicobacter pylori are spiral-shaped, Gram-negative, microaerophilic bacteria with flagella that develop gastritis, gastric ulcer, and gastric adenocarcinoma, as well as are associated with primary gastric lymphoma and expansion within the glandular epithelium [1,2]. H. pylori infection often constantly causes chronic swelling, but only some of the Helicobacter strains can create ulcer more than the others.…”

Section: Introductionmentioning

confidence: 99%

Fecal Helicobacter pylori glmM and 16S rRNA genes correlate with serum TNF-α and IL-1β cytokine fluctuations

Moradipour

Khosravi

Piri

2018

AMicr

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The proinflammatory cytokines of TNF-α and IL-1β have been reported to be increased in gastric mucosal surfaces in people with Helicobacter pylori infection. Accordingly, this study was conducted to investigate the relationship between the presence of H. pylori genes and the serum oscillations of these cytokines. In this study, DNA was first extracted from the stool samples of infected individuals and used as DNA template to investigate the presence of glmM and 16S rRNA genes in PCR. The ELISA assay was employed to examine serum levels of TNF-α and IL-1β cytokines. According to statistical analysis, there was a significant correlation between the presence of glmM and 16S rRNA genes in the stool samples of infected persons and the serum oscillations of TNF-α and IL-1β cytokines. At the end of study and analysis of the data in case group with HPSAg+, 47.6% of the glmM gene and 23.6% of the 16S rRNA gene were positive. In addition, a significant correlation was observed between the presence of glmM and 16S rRNA genes in the stool specimens of infected individuals and the serum levels of TNF-α and IL-1β cytokines (p < 0.05). Considering the results, it can be concluded that fluctuations in the amount of HPSA, TNF-α, and IL-1β in H. pylori infection depend on the presence of glmM and 16S rRNA genes. The presence of glmM and 16S rRNA in the stool sample increases by boosting the response level to stool antigen (HPSA), IL-1β, and TNF-α, suggesting the prognosis of the disease with a bacterial virulence form using stool tests.

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Correlation of 16s rRNA with serum levels of the cytokines, TNF-α and IL-1β, in subjects with a positive Helicobacter pylori Stool Antigen test (HPSA)

Cited by 3 publications

References 17 publications

Fucoidan from Sargassum hemiphyllum inhibits infection and inflammation of Helicobacter pylori

Fucoidan from Sargassum hemiphyllum inhibits infection and inflammation of Helicobacter pylori

Fucoidan from Sargassum Hemiphyllum Inhibits Infection and Inflammation of Helicobacter Pylori

Fecal Helicobacter pylori glmM and 16S rRNA genes correlate with serum TNF-α and IL-1β cytokine fluctuations

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