Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media

Abstract

Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.

Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico

“…Mastitis also has negative effects on the quality of the milk and manufactured milk products, decreasing the shelf life of the liquid milk products (Guerin-Faublee et al 2002). Intramammary infections with mastitis has negative effects resulting in the reduction of milk yield (Souto et al 2010;Fragkou et al 2014) which is considered to be the most important cause of worldwide economic losse in the dairy industry. The most frequently isolated microorganisms are staphylococci, streptococci, and coliforms, but other microorganisms may infect the udder (Blum et al 2014;Hosseinzadeh and Saei 2014).…”

Mastites em ruminantes no Brasil

“…(Bianchini et al 2010) 84,4 4,4 4,4 4,4 2,2 B (Neves et al 2010) 83,3 16,7 B (Peixoto et al 2010a) 29,5 27,1 25,7 2,9 7,1 2,9 4,8 B (Silva et al 2011) 90,3 4,9 1,7 1,7 B (Silva et al 2013) 61,5 3,07 13,9 6,16 9,2 C (Medeiros et al 2013) 49 Souto et al 2010) 29 ). Nos últimos anos, os Staphylococcus Coagulase Negatica (SCN) são isolados com maior frequência a partir de amostras de animais com mastite em diferentes países , Schukken et al 2009, Fessler et al 2010.…”

“…Este microrganismo na maioria dos casos de infecção intramamária é sensível a diferentes antimicrobianos (Gao et al 2012, Krishnaveni et al 2014. No Brasil, os Streptococcus ambientais mais frequentemente isolados são S. dysgalactiae e S. uberis (Souto et al 2010).…”

Effect of Parenteral Supplementation with a Mineral Compound of Cu, Zn, Mn on Bovine Mastitis

“…From a practical perspective, mastitis is the response of the gland to infection commonly acquired by invasion of pathogenic bacteria through the orifice and teat canal. This disease is an inflammatory process of the mammary gland that causes changes in the composition of milk, the secretion of the epithelial cells and reduced functional capacity of the udder [1]. It is the disease with the most prevalence in dairy herds [2], with a great economic impact on milk production [3].…”