Correlation between key enzyme activities in the inosine synthetic pathway and inosine production

Chu, Ju; Zhang, Siliang; Zhuang, Yingping; Song, Yongbo; Cai, Xiwen

doi:10.1016/j.procbio.2004.02.023

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…5), and was inhibited strongly and specifically by purine compounds (Chu et al, 2005). The accumulation of PRPP also decreased the PRRR synthetase activity under the same conditions (Henderson et al, 1975).…”

Section: Screening Of 8-ag-resistant Mutantsmentioning

confidence: 90%

“…New substance formed after implantation might affect the activity of enzymes involved in cAMP metabolism (Gu et al, 2006). IMP dehydrogenase is an important enzyme in the ribonucleotide synthetic pathway and converts IMP to GMP (Chu et al, 2005). The presence of this enzyme adversely affects accumulation of cAMP.…”

Section: Changes In Key Enzyme Activities In Camp Biosynthesismentioning

confidence: 99%

See 1 more Smart Citation

Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Song

Chen

Cao

et al. 2010

Radiation Physics and Chemistry

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Section: Screening Of 8-ag-resistant Mutantsmentioning

confidence: 90%

Section: Changes In Key Enzyme Activities In Camp Biosynthesismentioning

confidence: 99%

Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Song

Chen

Cao

et al. 2010

Radiation Physics and Chemistry

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“…Considering the carbon balance, it was deduced that some intermediates of metabolism, such as amino acids, organic acids, or some other nitrogenous substances, had accumulated, and the metabolic flux had shifted to synthesis of these intermediates, other than guanosine. As evidenced by the time-course of by-products, key enzymatic activities, and stoichiometric calculation of metabolic flux shift, it was concluded that late-phase production of guanosine competed against alanine accumulation, which provided possible direction for metabolic engineering and also for process optimization [6,34,35]. Another successful case was the optimization of recombinant human serum albumin by an engineered P. pastoris.…”

Section: Process Optimization In the Context Of Association Analysis mentioning

confidence: 99%

Advances and Practices of Bioprocess Scale-up

Xia

Wang

Lin

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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: This chapter addresses the update progress in bioprocess engineering. In addition to an overview of the theory of multi-scale analysis for fermentation process, examples of scale-up practice combining microbial physiological parameters with bioreactor fluid dynamics are also described. Furthermore, the methodology for process optimization and bioreactor scale-up by integrating fluid dynamics with biokinetics is highlighted. In addition to a short review of the heterogeneous environment in large-scale bioreactor and its effect, a scale-down strategy for investigating this issue is addressed. Mathematical models and simulation methodology for integrating flow field in the reactor and microbial kinetics response are described. Finally, a comprehensive discussion on the advantages and challenges of the model-driven scale-up method is given at the end of this chapter.

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“…Inosine (C 10 H 12 N 4 O 5 ; CAS no. 58-63-9; molecular weight, 268.23 g·mol –1 ; IUPAC name, 9-((2 R ,3 R ,4 S ,5 R )-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-3 H -purin-6-one; molecular structure shown in Figure S1 of the Supporting Information) is known as hypoxanthine or hypoxanthine nucleoside, which is a nucleoside derived from a purine derivative antimetabolite, containing both a polar furan ring and an apolar aromatic group. − As we know, nucleoside derivatives have been extensively investigated due to their potential activity as antibiotics, enzyme inhibitors, anticancer, antiviral agents, and ATP generation. − It is an important component of ribonucleic acids, with the hydrophilic–hydrophobic balance of the molecule contributing to their conformational stability, which has a great impact role on a large number of various chemical and biological reactions and other areas. ,, …”

Section: Introductionmentioning

confidence: 99%

Solubility Behavior and Thermodynamic Modeling of Inosine (Form β) in Four Cosolvency Systems at T = 278.15 to 323.15 K

2020

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The N,N-dimethylformamide (DMF) as the main solvent with strong dissolving power was blended with four secondary solvents (ethanol, n-propanol, isopropanol, propylene glycol (PG)) with relatively weak dissolving power to form many new solvents. The dispersion index (Inosine (form β) mole fraction) in five organic solvents such as DMF and in the newly formed solvent was also obtained one by one with static method commonly used in solid-liquid equilibrium. The temperature environment is that the high temperature was set at 323.15 K, and the low temperature was set at 278.15 K and the interval between each temperature was 5 K. The pressure environment was the atmospheric pressure in the natural state and the usual value was 101.0 kPa. In a mixed system, temperature was a non-negligible influencing factor from beginning to end and its increase often leaded the solute to the trend of high solubility. In addition, the proportion of the main solvent also dominated the solubilization trend of the inosine (form β), the larger the proportion of DMF, the easier the dissolution process was. When both of the above factors were fixed at a certain point, the dispersing ability of the dispersing liquid composed of DMF and ethanol was undoubtedly the first. Three models (the Jouyban-Acree model, van't Hoff-Jouyban-Acree model and Modified Apelblat-Jouyban-Acree model) were used to correlate the solubility data. The largest RAD and RMSD were 4.66×10 -2 and 7.27×10 -4 . The dispersion data of inosine (form β) obtained through this experimental process and related thermodynamic parameters obtained through thermodynamic calculations have important application significance for its industrial production and further purification. ■ INTRODUCTIONFrom the history of the development of chemicals, there are many methods for the purification of drugs, such as membrane separation, supercritical fluid technology and so on, but the dissolution crystallization technology is undoubtedly the most efficient and cheapest one and the most widely used one. [1][2][3][4][5] Especially for poorly water-soluble chemicals, how to increase the solubility is a

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Correlation between key enzyme activities in the inosine synthetic pathway and inosine production

Cited by 3 publications

References 5 publications

Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Advances and Practices of Bioprocess Scale-up

Solubility Behavior and Thermodynamic Modeling of Inosine (Form β) in Four Cosolvency Systems at T = 278.15 to 323.15 K

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