Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Song, He; Chen, Xiaochun; Cao, Jiaming; Fang, Ting; Bai, Jianxin; Xiong, Jing; Ying, Hanjie

doi:10.1016/j.radphyschem.2010.03.005

Cited by 13 publications

(9 citation statements)

References 20 publications

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“…Mutation breeding is a common method to obtain high mutation efficiency . Chemical mutagenesis is a useful tool to study the physiological processes in microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits

Liang

et al. 2014

J Sci Food Agric

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“…Mutation breeding is a common method to obtain high mutation efficiency . Chemical mutagenesis is a useful tool to study the physiological processes in microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits

Liang

et al. 2014

J Sci Food Agric

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“…Low-energy ion beam implantation, as a new mutagenesis technique [1], has attracted much attention in breeding of microorganisms and crops for its advantages, such as higher mutation rate, wider spectrum and lower injury rate of mutation than the traditional mutation methods [2]. Many high-yield strains have been obtained by ion beam implantation in industrial microorganisms [3,4].…”

Section: Introductionmentioning

confidence: 99%

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Zhaoyang

et al. 2011

Indian J Microbiol

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In this paper, in order to obtain some industrial strains with high yield of L-(?)-lactic acid, the wild type strain Lactobacillus casei CICC6028 was mutated by nitrogen ions implantation. By study, it was found that the high positive mutation rate was obtained when the output power was 10 keV and the dose of N ? implantation was 50 9 2.6 9 10 13 ions/cm 2 . In addition, the initial screening methods were also studied, and it was found that the transparent halos method was unavailable, for some high yield strains of L-(?)-lactic acid were missed. Then a mutant strain which was named as N-2 was isolated, its optimum fermentation temperature was 40°C and the L-(?)-lactic acid yield was 136 g/l compared to the original strain whose optimum fermentation temperature was 34°C and L-(?)-lactic acid production was 98 g/l. Finally, High Performance Liquid Chromatography method was used to analyze the purity of L-(?)-lactic acid that was produced by the mutant N-2, and the result showed the main production of N-2 was L-(?)-lactic acid.

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“…This was essentially different from the exponential curve for which the survival rate decreased with the increasing of radiation dose, such as proposed by UV and 60 Co -rays [19][20]. The saddle-shape curve was regarded as the typical and important mutation effect of ion implantation in many researches [4,8,[16][17][18], and was also proven by our experimental results. According to the theory of saddle-shape curve by Song [21], the optimum dose for hbs1 was 1×10 14 ion/cm 2 , while the survival rate was 25.5%.…”

Section: Effect Of N + Implantation On Survival Rate Of Hbs1mentioning

confidence: 48%

Mutagenesis and screening of highly efficient pyrene degrading strains by N<sup>+</sup>-implantation

Meng

Jin

2011

2011 International Conference on Remote Sensing, Environment and Transportation Engineering

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In this study, nitrogen ion implantation was used to mutate a pyrene-degrading bacterium, Gordona sp. hbs1, which was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil. The pyrene degradation characteristics of hbs1, including isotherm and pH effect were observed. 200 mg/L pyrene, 53.78% was degraded by hbs1, equals to 10.75 mg/L. The optimum initial pH for pyrene degradation was 7 to 7.5. The mutagenesis was conducted by 30 KeV nitrogen ion implantation with the doses of 5×10 12 , 1×10 13 , 2×10 13 , 4×10 13 , 5×10 13 , 1×10 14 , 2×10 14 , 5×10 14 ions/cm 2 . The results showed that the optimum dose was 1×10 14 ions/cm 2 . Three highly pyrene degrading strains, hbs1-m11, hbs1-m23 and hbs1-m48 were obtained and their increased efficiencies were 18.72%, 10.71% and 13.10%, respectively. Furthermore, pyrene degrading rate of hbs1-m11 was higher than hbs1. These results imply that ion implantation could be a potential technology in environmental bioremediation .

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Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

Cited by 13 publications

References 20 publications

Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits

Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Mutagenesis and screening of highly efficient pyrene degrading strains by N<sup>+</sup>-implantation

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