Correlation between fusaric acid production and virulence of isolates ofFusarium oxysporum that causes potato dry rot in South Africa

Venter, S.L.; Steyn, P. J.

doi:10.1007/bf02358198

Cited by 47 publications

(43 citation statements)

References 9 publications

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“…The results of the present work support those of Venter et al [32], who also demonstrated a signiWcant correlation between the virulence of Fusarium oxysporum isolates and the production of FA in potato during the development of dry rot. The Wndings of Notz et al [27] support those of the present work, since they showed that FA secretion into artiWcial media may diVer according to the isolate and the composition of the media.…”

Section: Pathogenicity Testsupporting

confidence: 94%

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Impact of biological control agents on fusaric acid secreted from Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen in Gladiolus grandiflorus corms

Nosir

McDonald

Woodward

2010

J Ind Microbiol Biotechnol

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Fusaric acid (FA) (5-n-butylpuridine 2-carboxyl acid), a highly toxic secondary metabolite produced by Fusarium oxysporum strains, plays a significant role in disease development. The abilities of three F. oxysporum f. sp. gladioli (Massey) Snyder and Hansen isolates (G010; 649-91; and 160-57) to produce FA in infected Gladiolus corm tissues was evaluated in vitro in relation to the presence of two biological control agents, Trichoderma harzianum T22, and Aneurinobacillus migulanus. Pathogenicity tests were used to differentiate between the abilities of the F. oxysporum strains to secrete FA. FA was identified using LC/MS and quantified using HPLC. Isolate G010 was significantly more virulent (P < 0.01) on Gladiolus grandiflorus corms; it secretes 1.8 μM FA/g fresh weight corm into inoculated Gladiolus. Moreover, G010 was the only isolate that produced FA among the three examined isolates. There was a correlation between the corm lesion area and the FA secretion ability of F. oxysporum f. sp. gladioli (P < 0.001; r (2) = 0.96). No FA was detected in PDA cultures of F.oxysporum f. sp. gladioli isolates. The presence of T. harzianum T22 appeared to prevent FA secretion into the corms. In the presence of A. migulanus, however, the amount of FA secreted into the corm tissues increased. These results support the use of T. harzianum as an effective biological control agent against F. oxysporum f. sp. gladioli.

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Section: Pathogenicity Testsupporting

confidence: 94%

“…Pathogenic and nonpathogenic Fusarium strains produce fusaric acid (FA; 5-butylpicolinic acid) [2,27,29]. FA is toxic to prokaryotes and eukaryotes [5,32]. In addition, FA was shown to be involved in fungal defence against biological control strains of Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Impact of biological control agents on fusaric acid secreted from Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen in Gladiolus grandiflorus corms

Nosir

McDonald

Woodward

2010

J Ind Microbiol Biotechnol

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“…Fusaric acid is one of the earliest discovered fungal metabolites with a proposed role in pathogenesis, since it causes wilting [196]. A large body of correlative evidence is available supporting a role in virulence (e.g., [197,198]), but due to a lack of correlation between loss of fusaric acid production in UV-mutagenized F. oxysporum and virulence this hypothesis has been largely dismissed [199]. A role of fusaric acid has been proposed again in recent literature, for instance in banana wilt [200] or vascular wilt of pea by F. oxysporum f. sp.…”

Section: Fusaric Acidmentioning

confidence: 99%

Fusarium Mycotoxins and Their Role in Plant–Pathogen Interactions

2015

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“…FA was extracted and purified from the culture filtrate of Fusarium oxysporum f.sp. lycopersici (causal agent of Fusarium wilt of tomato) [16]. The purified and characterized FA was stored at 4°C until further use.…”

Section: A Plant Materials and Fusaric Acid Treatmentmentioning

confidence: 99%

“…Control plants were infiltrated with sterile distilled water. Plants were harvested at 0, 4,8,12,16,20,24,36,48 and 72 h after FA treatment.…”

Section: A Plant Materials and Fusaric Acid Treatmentmentioning

confidence: 99%

Induction of Defence Responses by Fusaric Acid (Fusarium Toxin) in Tomato Plant

2016

6th International Conference on Advances in Engineering Sciences and Applied Mathematics (ICAESAM-2016) Dec. 21-22, 2016 Kuala

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Abstract-Fusaric acid (FA) is a host non-specific toxin produced by a number of Fusarium species. The most expansive producer of this toxin is Fusarium oxysporum and its special forms (f. sp.) lycopersici (causal agent of Fusarium wilt of tomato). FA is suspected to be a potent pathogenicity factor in tomato wilt disease development. With this rationale the present study was carried out with biochemical and molecular perspectives. In the present study, the treatment of FA was given to the leaves of tomato directly through infiltration to assess the induction of defence responses. The phytotoxic effect of FA was assessed in the form of cell death in tomato leaves which was observed by increased uptake of Evans blue stain. This cell death inducer (FA) produced an enormous oxidative burst during which large quantities of reactive oxygen species (ROS) like H 2 O 2 was generated in the treated leaf tissues of tomato plants which was evident from enhancement in lipid peroxidation. However, to cope with the toxicity of FA, tomato plants develop a cellular strategy involving activation of antioxidative defence system. This modulation of antioxidative system could reflect a defence response to the cellular damage provoked by treatment of FA. Results demonstrate that production of phytohormone (SA) potentiates the oxidative burst, while ROS increases its production. In this way these signaling molecules stimulate each other's production, thus amplifying the initial signal, ultimately leading to plant cell death. A significant increase of PAL activity in tomato plants confirmed the responsiveness of PAL-mediated primary defence system against the FA exposure. In the present study, the level of total phenols (important component of plant defence machinery) increased significantly in consonance with increase in the ROS level.Furthermore, proteomic study was used as a powerful tool to understand the alterations in cellular protein expression in response to FA exposure. Differential expression in several proteins was observed in the present study. The present study provides clues to understand the biochemical and molecular changes that occurred in the host plant, Solanum lycopersicum L., during the FA treatment. The study provides valuable outputs regarding vulnerability of such vegetable crop plant under FA exposure, which can be used in future breeding to design effective ameliorative strategy for the generation of resistant plants.

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Correlation between fusaric acid production and virulence of isolates ofFusarium oxysporum that causes potato dry rot in South Africa

Cited by 47 publications

References 9 publications

Impact of biological control agents on fusaric acid secreted from Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen in Gladiolus grandiflorus corms

Impact of biological control agents on fusaric acid secreted from Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen in Gladiolus grandiflorus corms

Fusarium Mycotoxins and Their Role in Plant–Pathogen Interactions

Induction of Defence Responses by Fusaric Acid (Fusarium Toxin) in Tomato Plant

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