Correlation  between estrogen and alkaline phosphatase expression in osteoporotic rat model

Salim, Sherman

doi:10.20473/j.djmkg.v49.i2.p76-80

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…45,46 The coupling reaction between the RANK receptor expressed by osteoclasts and RANK ligand secreted by osteoblasts enhances the maturation and activity of osteoclast cells thus, increasing bone resorption. [47][48][49] Excessive bone resorption in postmenopausal osteoporosis is characterized by elevated serum ALP activity, osteocalcin, and RANKL levels in the estrogen-deficient rat model. [50][51][52] Interestingly, chrysin significantly suppressed the increased of BALP activity, osteocalcin, and RANKL levels in OVX rats when compared to untreated OVX rats.…”

Section: Discussionmentioning

confidence: 99%

Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico

et al. 2021

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Background Evidences are beginning to accrue that flavonoids, particularly phytoestrogens, could have beneficial effects against several age-related diseases linked to estrogen deficiency including postmenopausal osteoporosis. Methods In this study, the effect of chrysin on selected bone-remodeling markers in ovariectomized rats and its estrogen-like activity in silico were investigated. Results The data indicated that administration of chrysin at 50 mg/kg and 100 mg/kg for 6 weeks to OVX rats significantly ( p < 0.05) prevented body weight gain and partially reverse uterine weight loss. In addition, treatment of OVX rats significantly ( p < 0.01) increased femur dry weight, femur ash weight, bone ash calcium, and phosphorous levels in a dose-dependent manner. However, there was significant ( p < 0.001) decline in serum estradiol level in all OVX rats compared to the sham-operated group. Interestingly, administration of chrysin significantly ( p < 0.05) reversed the reduction of estradiol induced by ovariectomy compared to untreated OVX rats. Moreover, administration of chrysin to OVX rats significantly ( p < 0.05) suppressed excessive elevation of bone-remodeling markers expression compared to untreated OVX rats. Similarly, molecular docking analysis revealed that chrysin interacts with both α and β estrogen receptors with exothermic binding energies of −229.83 kcal/Mol and −252.72 kcal/Mol, respectively, and also fits perfectly into the active site of both α and β estrogen receptors. Conclusion This study demonstrated that chrysin exhibits potential antiosteoporotic effects against bone loss in OVX rats through enhanced bone mineral contents and preventing excessive elevation of bone-remodeling markers and bone-resorbing cytokine.

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Section: Discussionmentioning

confidence: 99%

Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico

et al. 2021

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“…Osteoporotic women suffer from a low level of estrogen resulting in their bone density decreasing by 50% in trabecular bone and 35% in cortical bone. [910] Estrogen executes an important role in bone formation. Its deficiency can lead to imbalance in remodeling process due to a reduced ratio of osteoblast to osteoclast.…”

Section: Introductionmentioning

confidence: 99%

The effect of low-level estrogen in mandibular bone: An in vivo study

et al. 2019

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Background: Low levels of estrogen can cause osteoporosis and usually occur during a woman's menopausal phase. Osteoporosis can lead to bone resorption, the absence of osseointegration, and implant failure. The aim of this study is to determine the expression of transforming growth factor-beta 1 (TGF-β1), runt-related transcription factor (RUNX2), and osteoblasts in mandibular rats with low levels of estrogen. Materials and Methods: This study is an in vivo experimental research. Female Wistar rats ( n = 18) were divided into two groups: (1) Postsham surgery and (2) ovariectomy group. After 12 weeks, the rats were sacrificed to identify the level of estrogen, while histological analysis was conducted to determine the level of osteoblast and the expression of TGF-β1 and RUNX2. The data were analyzed using t -test ( P < 0.05). Results: There were significant lower levels of estrogen and osteoblast among the ovariectomy group compared to the postsham group ( P < 0.05). RUNX2 levels were found to be significantly higher in the ovariectomy group than that in the postsham group ( P < 0.05). However, there were no significant differences between TGF-β1 levels within the ovariectomy and postsham groups ( P > 0.05). Conclusion: Ovariectomy can lead to decreased osteoblastogenesis in mandibular bone by the reduced level of osteoblast and the increased expression of TGF-β1 and RUNX2.

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Risk Factors of Body Mass Index (BMI), Age of Menarche, Parity and Hormonal Contraception of Genu Osteoarthritis in Female Patients

Alisiya

Sulistiawati

Kurniawati

et al. 2021

FMI

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Osteoarthritis (OA) is a degenerative disease related to joint cartilage and commonly occurs in the knee joint. The 2013 National Survey recorded the prevalence of joint diseases in East Java was 26.9%. OA affected more women than men due to the estrogen and caused disabilities in many women. This study aimed to find the relationship between Body Mass Index (BMI), age of menarche, parity, and the use of hormonal contraceptives against OA in genu at Universitas Airlangga Hospital, Surabaya. This was an analytic observational study with a case-control approach. Data collection was carried out through a short interview using a questionnaire. The sample size was calculated using a formula and found that the study required 42 patients with OA in the case group and 42 patients without OA in the control group. The case group was dominated by patients with 56-60 years old age (62.1%), BMI 23-24.9 kg/m2 (58.7%), menarche age 12-13 years (53.6%), multiparous (52.6%), having contraception pill usage history (62.3%) particularly using combination pills (60.5%) with a mean duration of use > 1 year (56.8%). There was a relationship between BMI and type of hormonal contraceptive used against genu OA in female patients at Universitas Airlangga Hospital Surabaya.

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Correlation between estrogen and alkaline phosphatase expression in osteoporotic rat model

Cited by 3 publications

References 9 publications

Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico

Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico

The effect of low-level estrogen in mandibular bone: An in vivo study

Risk Factors of Body Mass Index (BMI), Age of Menarche, Parity and Hormonal Contraception of Genu Osteoarthritis in Female Patients

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