Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico

Abstract

Background Evidences are beginning to accrue that flavonoids, particularly phytoestrogens, could have beneficial effects against several age-related diseases linked to estrogen deficiency including postmenopausal osteoporosis. Methods In this study, the effect of chrysin on selected bone-remodeling markers in ovariectomized rats and its estrogen-like activity in silico were investigated. Results The data indicated that administration of chrysin at 50 mg/kg and 100 mg/kg for 6 weeks to OVX rats significantly ( p < 0.05) prevented body weight gain and partially reverse uterine weight loss. In addition, treatment of OVX rats significantly ( p < 0.01) increased femur dry weight, femur ash weight, bone ash calcium, and phosphorous levels in a dose-dependent manner. However, there was significant ( p < 0.001) decline in serum estradiol level in all OVX rats compared to the sham-operated group. Interestingly, administration of chrysin significantly ( p < 0.05) reversed the reduction of estradiol induced by ovariectomy compared to untreated OVX rats. Moreover, administration of chrysin to OVX rats significantly ( p < 0.05) suppressed excessive elevation of bone-remodeling markers expression compared to untreated OVX rats. Similarly, molecular docking analysis revealed that chrysin interacts with both α and β estrogen receptors with exothermic binding energies of −229.83 kcal/Mol and −252.72 kcal/Mol, respectively, and also fits perfectly into the active site of both α and β estrogen receptors. Conclusion This study demonstrated that chrysin exhibits potential antiosteoporotic effects against bone loss in OVX rats through enhanced bone mineral contents and preventing excessive elevation of bone-remodeling markers and bone-resorbing cytokine.

Chrysin Attenuates High Glucose-Induced BMSC Dysfunction via the Activation of the PI3K/AKT/Nrf2 Signaling Pathway

“… 14 Besides, chrysin was reported to protect ovariectomized rats against bone loss through enhancing bone mineral contents and inhibiting bone resorption. 15 However, the effect of chrysin on bone regeneration under T1DM conditions is still unclear as of yet.…”

Targeting bone homeostasis regulation: potential of traditional Chinese medicine flavonoids in the treatment of osteoporosis

“…Introducing the variable “estrogen” in studies on flavonoids to regulate the bone immune system would aid in comprehending the effects of TCM on postmenopausal conditions characterized by low estrogen levels and decreased immune function. For instance, molecular docking was used to confirm that chrysin, an extract derived from Oroxylum indicum (L.) Kurz in the family Bignoniaceae, interacts with both α and β estrogen receptors with exothermic binding energies of 229.83 kcal/Mol and 252.72 kcal/Mol, respectively, and also fits perfectly into the active site of both α and β estrogen receptors ( Ibrahim et al, 2021 ). The interaction between the drug and the receptor reversed the reduction in estradiol caused by ovariectomy.…”