Correlation Between Ciliary Beat Frequency and the Structure of Ciliated Epithelia in Pathologic Human Nasal Mucosa

Joki, Susanna; Toskala, Elina; Saano, Veijo; Nuutinen, Juhani

doi:10.1097/00005537-199803000-00021

Cited by 42 publications

(33 citation statements)

References 14 publications

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“…A decrease in the CBF was associated with ciliary axes having greater disorientation than the normal standard deviation (CZAJA and MCCAFFREY, 1998). Correlation between disorientation and CBF was also observed in patients with chronic and recurrent sinusitis (JoKI et al, 1998). Contrary to these results, it has been also reported that ciliary disorientation is correlated with mucociliary transport velocity, but not with CBF, in patients with chronic inflammatory sinusitis (RAYNER et al, 1995).…”

Section: Correlation Between C B F and M W Dmentioning

confidence: 75%

Correlation between ciliary beat frequency and metachronal wave disorder using image analysis method

Park

Lee

et al. 2003

Med. Biol. Eng. Comput.

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Ciliary beating and metachronal waves are fundamental to effective mucociliary transport. The ciliary beat frequencies (CBFs) and metachronal wave directions of multiple cilia beating in culture media were measured simultaneously using digital microscopic images. The degree of synchronisation between ciliary beats was determined by the correlation between ciliary signals at two different locations. The wave propagation directions of cilia were determined from a two-dimensional correlation map by a principal axis method. The standard deviation of measured wave directions in a region of interest was defined as a measure of metachronal wave disorder (MWD). Considerable variation was found in the beat frequencies and metachronal wave directions of cilia beating on epithelium. The pooled mean of MWDs was 23.4 +/- 8.8 degrees, and the pooled mean of CBFs was 10.1 +/- 1.9 Hz on 120 cells from five healthy subjects. The means of the MWD and the CBF from subjects were highly correlated (correlation = -0.83). The higher the CBF, the lower the level of the MWD.

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Section: Correlation Between C B F and M W Dmentioning

confidence: 75%

Correlation between ciliary beat frequency and metachronal wave disorder using image analysis method

Park

Lee

et al. 2003

Med. Biol. Eng. Comput.

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“…However, Joki et al [19] showed a correlation between ciliary orientation and CBF in a semi-quantitative manner using scanning electron microscopic images. This discrepancy can be attributed to the different methods used to measure ciliary disorientation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of ciliary wave disorders measured by image analysis and electron microscopy

Lee¹,

Kim

et al. 2005

Acta Oto-Laryngologica

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“…(2) An adequate mucociliary clearance is fundamental not only for the normal functioning of the lungs in a healthy state but also, and principally, in the presence of respiratory infections. (3) Previous studies have shown significant impairment of MCT after lung transplant.…”

Section: Methodsmentioning

confidence: 99%

Efeitos da azatioprina sobre a depuração mucociliar após secção e anastomose brônquica em um modelo experimental em ratos

et al. 2008

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Objective: To evaluate the effects of azathioprine on the mucociliary system in a model of bronchial section and anastomosis in rats. Methods: Thirty-six male Wistar-Furth rats were submitted to left bronchial section and anastomosis and divided into two groups to receive either saline solution or azathioprine. After 7, 15 and 30 days of treatment, six animals from each group were killed, after which in situ mucociliary transport velocity, in vitro mucus transportability, and contact angle of mucus in the right (intact) and left (sectioned) bronchi were measured. Results: In situ mucociliary transport velocity was significantly lower in the sectioned bronchi than in the intact bronchi (p < 0.001). In situ mucociliary transport velocity was lower in the intact bronchi of the animals treated with azathioprine for 7 days (p < 0.05), and those bronchi presented full recovery after 30 days of treatment. The contact angle was higher in the mucus samples collected from the sectioned bronchi of the animals treated with saline solution for 30 days (p < 0.001), which is in accordance with the decreased in vitro mucus transportability observed in the same animals (p < 0.001). Conclusions: We conclude that, in the sectioned bronchi of rats, treatment with azathioprine causes only transitory impairment of mucociliary transport, whereas administration of saline solution impairs mucociliary transport for up to 30 days. In addition, azathioprine protects against alterations in mucus surface properties.Keywords: Mucociliary clearance; Anastomosis, surgical; Immunosuppression; Lung. ResumoObjetivo: Avaliar os efeitos da azatioprina sobre o sistema mucociliar em um modelo de secção e anastomose brônquica em ratos. Métodos: Trinta e seis ratos machos da raça Wistar-Furth foram submetidos à secção e anastomose brônquica esquerda e separados aleatoriamente em dois grupos para receberem solução salina ou azatioprina. Após 7, 15 e 30 dias de terapia, seis animais de cada grupo foram sacrificados, e foram realizadas as medidas da velocidade de transporte mucociliar in situ, da transportabilidade do muco in vitro e do ângulo de contato do muco nos brônquios direito (intacto) e esquerdo (seccionado). Resultados: A velocidade de transporte mucociliar in situ foi significativamente menor nos brônquios seccionados do que nos brônquios intactos (p < 0,001). Houve redução da velocidade de transporte mucociliar in situ nos brônquios intactos dos animais tratados com azatioprina por 7 dias (p < 0,05), havendo completa recuperação após 30 dias de terapia. O ângulo de contato do muco foi maior nos brônquios seccionados dos animais tratados com solução salina por 30 dias (p < 0,001), estando de acordo com a redução da transportabilidade do muco in vitro observada nos mesmos animais (p < 0,001). Conclusões:Concluímos que, nos brônquios seccionados de ratos, a terapia com azatioprina causa um prejuízo apenas transitório do transporte mucociliar, enquanto a administração de solução salina prejudica o transporte mucociliar por até 30 dias. Além...

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Correlation Between Ciliary Beat Frequency and the Structure of Ciliated Epithelia in Pathologic Human Nasal Mucosa

Cited by 42 publications

References 14 publications

Correlation between ciliary beat frequency and metachronal wave disorder using image analysis method

Correlation between ciliary beat frequency and metachronal wave disorder using image analysis method

Comparison of ciliary wave disorders measured by image analysis and electron microscopy

Efeitos da azatioprina sobre a depuração mucociliar após secção e anastomose brônquica em um modelo experimental em ratos

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