Correction of metabolic processes in rats during chronic endotoxicosis using isotope (D/H) exchange reactions

Джимак, С. С.; Басов, А. А.; Fedulova, Liliya; Didikin, A. S.; Bikov, I. M.; Arcybasheva, O M; Naumov, George N.; Барышев, М. Г.

doi:10.1134/s1062359015050064

Cited by 20 publications

(15 citation statements)

References 15 publications

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“…Intermolecular and intramolecular kinetic isotope effects are summarized in biological objects due to complexity of high-molecular compounds (proteins, nucleic acids) and their solvation capacity, and hence the isotopic exchange reactions of hydrogen between chemical dissociating groups and hydration shell of macromolecules. Such changes lead to fluctuation of energy during an enzyme-substrate interaction (intermolecular effect) and can be accompanied by different rates of intramolecular conformational rearrangements (enzyme-substrate complex) (16). These effects may lead to a decrease of time within the individual stage of biocatalytic transformation or acceleration recovery of enzyme to active form after single catalytic cycle, thereby increasing the activity of biochemical processes (17).…”

Section: Discussionmentioning

confidence: 99%

“…Deuterium-depleted water (DDW) is 1 H 2 16 O water isotopologue formed by light stable isotopes of hydrogen and oxygen. Furthermore, international standard mean ocean water (SMOW) and SLAP recorded the basic standards of isotopic composition in hydrosphere, including concentration of water molecules containing heavy isotopes (D, 17 O, 18 O).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Сергеевич

Басов

Елкина

et al. 2018

Jundishapur J Nat Pharm Prod

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The objective of the current study was to evaluate the influence of deuterium-depleted water (DDW) consumption on healthy animals and animals with hepatorenal toxicity. The influence of deuterium-depleted water on hepatorenal toxicity was investigated in Wistar rats. Deuterium-depleted water consumption during 42 days caused changes in isotopic D/H composition of rats' plasma and lyophilized tissues of internal organs (liver and kidney). Moreover, deuterium-depleted water influenced plasma biochemical parameters. Concentration of creatinine and bilirubin, as well as aspartate aminotransferase and alanine aminotransferase activities were reduced in plasma of rats with hepatorenal toxicity. Deuterium-depleted water consumption during 42 days influenced the body and internal organ weights while integral index of chronic intoxication did not increase in rats with chronic intoxication. It was also revealed that deuterium-depleted water consumption did not display any toxic effects.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Сергеевич

Басов

Елкина

et al. 2018

Jundishapur J Nat Pharm Prod

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“…Water with low deuterium intervented to the water menu of the laboratory animals changes the isotopic metabolism rate, and this has an effect on the thermokinetic and thermodynamic biomolecules parameters, accelerating biochemical processes depending on an isotopic composition of enzymes and coenzymes [6]. These kind of change, connected with an isotopiс metabolism reaction, can increase the adaptation potency of an organism by a stimulating of protection systems (antioxidant, for example) [7][8][9], and this can be used in a complex therapy of hepatic diseases.…”

Section: Introductionmentioning

confidence: 99%

Changes in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the water

Быков

Chikhladze

Щава

et al. 2018

Vestn. Ross. univ. družby nar., Ser. Med.

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Abstract. The aim of the research was to study the effect of the water menu with a low D/H on morphological hepatocytes parameters in rabbits in modeling of an acute hypoxic liver damage. Methods: morphological research of liver tissues of the experimental animals had been done by cytological and histological methods, which had been prepared impression smear of the studying organ after which it had been stained with azur-eosin according to a common procedure; the blood plasma had been done by the pulsing nuclear magnetic resonance spectrometer JEOL JNM-ECA 400MHz, evaluate an isotopic composition of rat lyophilized liver tissues had been used an isotopic mass-spectrometer DELTA plus . Results:There are the statistics presented in the given study reveal the feature of the water menu with the low deuterium concentration decreases the liver tissue damage in hypoxia simulating by the compression of a hepatoduodenal ligament. It was demonstrated that the use of the deuterium depleted water (DDW) reliably decreases the deuterium concentration in a blood plasma (by 32%) and in liver tissue (by 9%), which shows the effectiveness of the correlation of isotopic composition of internal environment and organs by the products with the low deuterium concentration.Conclusion: It has been established that the use of the DDW as a hepatoprotective measure is equal in its effectiveness to some of lipophilic antioxidants. This allows to use vegetative antioxidant factors and a water menu with a low deuterium concentration (40 ppm) as a combined measure, in order to correct morphological liver defects in its ischemic damage.

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“…According to analyzed material there were chosen methods aimed to biomolecules selection from animal raw material, comprising water-salt extraction, filtration and ultrafiltration, then lyophilic drying, with pretreated test specimens in all these stages by solutions on the basis of water with modified isotopic D/H compound (WMIC) [16,17]. Comparative study of WMIC selectivity and total extractability carried out in connection with recent publications on the water isotopic composition influence in the biological properties of proteins and peptides to change living systems adaptation properties [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

Fedulova

Vasilevskaya

Kotenkova³

et al. 2017

J. Pharm. Nutr. Sci.

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The influence of protein-peptide compounds, extracted with deuterium water as solubilizer agent from mixture of Sus scofa thymus, spleen and lymph nodes extracts, was carried out on Wistar rats with cytostatic-induced immunodeficiency model. Intragastric administration of extract fraction with molecular weight more than 30 kDa, did not lead to significant changes. Thus, polypeptide compounds with molecular weight less than 30 kD, extracted from Sus scofa immune organs, significantly increased T-lymphocytes amount, affected macrophages system by T-helper (CD4) stimulation and activated cycle of complementary cascade. Differences between the experimental results in T-helper synthesis activation (CD4) in animals treated with the medium and low molecular weight fractions can be explained by the ability of biomolecules having a molecular weight less than 30 kDa (in particular, isolated from the spleen, which, in its cellular structure contains key regulatory factors) to change the speed of cell differentiation.

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Correction of metabolic processes in rats during chronic endotoxicosis using isotope (D/H) exchange reactions

Cited by 20 publications

References 15 publications

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Changes in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the water

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

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