Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells

An, Yun Ah; Hwang, Jeoung Yeon; Lee, Jae Soon; Kim, Young Chul

doi:10.5487/tr.2015.31.2.165

Cited by 24 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the present study revealed that C. fructus significantly inhibited CYP2E1 expression and activity. Previous studies suggested that C. fructus contained many active components, such as flavones (quercetin and kaempferol), organic acids, phenolic acids (gallic acid and tannic acid), iridoid glycosides, saponins, and other chemical components [29,30]. In the present study, the methanolic extract of C. fructus is rich in phenols (153.88 ± 1.26 mg/g) and flavones (51.69 ± 0.66 mg/g).…”

Section: Discussionmentioning

confidence: 61%

Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice

et al. 2016

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 61%

Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Total RNA was isolated using the TriZol reagent (Life Technologies; Gaithersburg, MD), and the cDNA was prepared using M-MLV reverse transcriptase (Gibco-BRL; Gaithersburg, MD) according to the manufacturer's instructions [39] , [40] . The following primers were used for the amplification of human c-FLIP, Mcl-1, PSMA5, and actin: c-FLIP (sense) 5′- CGG ACT ATA GAG TGC TGA TGG -3′ and (antisense) 5′- GAT TAT CAG GCA GAT TCC TAG -3′, Mcl-1 (sense) 5′- GCG ACT GGC AAA GCT TGG CCT CAA-3′ and (antisense) 5′- GTT ACA GCT TGG ATC CCA ACT GCA-3′, PSMA5 (sense) 5′- CTT GCA AGA AGT TTA TCA CAA GTC T -3′ and (antisense) 5′- GAA ATT CTG GCC AGG CTG C -3′, and actin (sense) 5′- GGC ATC GTC ACC AAC TGG GAC -3′ and (anti-sense) 5′- CGA TTT CCC GCT CGG CCG TGG -3′.…”

Section: Methodsmentioning

confidence: 99%

NOX4-mediated ROS production induces apoptotic cell death via down-regulation of c-FLIP and Mcl-1 expression in combined treatment with thioridazine and curcumin

Seo

Kim

et al. 2017

Redox Biology

View full text Add to dashboard Cite

Thioridazine is known to have anti-tumor effects by inhibiting PI3K/Akt signaling, which is an important signaling pathway in cell survival. However, thioridazine alone does not induce apoptosis in head and neck squamous cell carcinoma (AMC-HN4), human breast carcinoma (MDA-MB231), and human glioma (U87MG) cells. Therefore, we investigated whether combined treatment with thioridazine and curcumin induces apoptosis. Combined treatment with thioridazine and curcumin markedly induced apoptosis in cancer cells without inducing apoptosis in human normal mesangial cells and human normal umbilical vein cells (EA.hy926). We found that combined treatment with thioridazine and curcumin had synergistic effects in AMC-HN4 cells. Among apoptosis-related proteins, thioridazine plus curcumin induced down-regulation of c-FLIP and Mcl-1 expression at the post-translational levels in a proteasome-dependent manner. Augmentation of proteasome activity was related to the up-regulation of proteasome subunit alpha 5 (PSMA5) expression in curcumin plus thioridazine-treated cells. Combined treatment with curcumin and thioridazine produced intracellular ROS in a NOX4-dependent manner, and ROS-mediated activation of Nrf2/ARE signaling played a critical role in the up-regulation of PSMA5 expression. Furthermore, ectopic expression of c-FLIP and Mcl-1 inhibited apoptosis in thioridazine and curcumin-treated cells. Therefore, we demonstrated that thioridazine plus curcumin induces proteasome activity by up-regulating PSMA5 expression via NOX4-mediated ROS production and that down-regulation of c-FLIP and Mcl-1 expression post-translationally is involved in apoptosis.

show abstract

“…Sodium dodecyl sulfate, however, is a detergent with no reported antibacterial activity [18]. Other components, such as pegylated hydrogenated castor oils, are detergents used in the cosmetic industry with no reported antibacterial activity [19] but a negative impact on keratinocyte viability [20]. Cetylpyridinium chloride possesses antimicrobial activity, is used in oral rinses [21, 22], and has a negative impact on L929 fibroblast viability [23].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Antimicrobial Activity of Oral Rinses In Vitro

Müller

Eick

Moritz

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

While oral rinses used for cosmetic purposes only do not necessarily have to be antiseptic, antimicrobial activity is required for medical indications, including oral and periodontal surgery. So the question arises—is the antimicrobial activity of oral rinses associated with any destructive changes in cell viability in vitro? To answer this question, we examined twelve oral rinses with respect to their antimicrobial and cytotoxic activity. Antimicrobial activity was screened against five bacterial strains using disc diffusion. Cytotoxicity was determined by mitochondrial reductase activity with primary gingival fibroblasts, L929 cells, and HSC-2 epithelial cells. Phase contrast microscopy and trypan blue staining were then performed to reveal cell morphology. Cells remained vital after exposure to oral rinses that were only used for cosmetic purposes. Moderate cytotoxic effects were observed for oral rinses containing 0.05% chlorhexidine, ethanol, or pegylated hydrogenated castor oil and sodium dodecyl sulfate. Other oral rinses containing 0.2% chlorhexidine and cocamidopropyl betaine exhibited strong cytotoxic and antimicrobial activity. Strong cytotoxic but moderate antimicrobial activity was observed in oral rinses containing cetylpyridinium chloride. The in vitro data show that oral rinses are heterogeneous with respect to their cytotoxic and antimicrobial effects. Based on their respective properties, oral rinses can be selected either to reduce the microbial load or for cosmetic purposes.

show abstract

Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells

Cited by 24 publications

References 28 publications

Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice

Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice

NOX4-mediated ROS production induces apoptotic cell death via down-regulation of c-FLIP and Mcl-1 expression in combined treatment with thioridazine and curcumin

Cytotoxicity and Antimicrobial Activity of Oral Rinses In Vitro

Contact Info

Product

Resources

About