Cordycepin production by a novel endophytic fungus Irpex lacteus CHG05 isolated from Cordyceps hawkesii Gray

Overproduction of cordycepin in Saccharomyces cerevisiae by cordycepin synthase screening and metabolic engineering

“…Cordyceps hawkesii Gray, which belongs to the Phlebia species and can produce 162.05 mg L À1 COR. 37 13 Like E. atlantica, Aspergillus hiratsukae has not been previously reported to produce COR-related compounds, but the genes it possesses indeed have functions related to COR production. By the results of multiple sequence comparisons, we found that about 60 amino acid residues starting at the N-terminal end of enzyme 1 may affect its activity for COR synthesis, since no COR production was detected in the strain SAC1 containing AC1 with an absence of this sequence.…”

“…Masuda et al obtained a C. militaris mutant G81‐3 with high COR content, and they controlled the optimal concentration of adenosine in the liquid medium by adding the precursor substance adenosine, which resulted in a maximum yield of COR of 8.6 g/L 22 . An endophytic fungi identifed as I. lacteus CHG05 was isolated from Cordyceps hawkesii Gray , which belongs to the Phlebia species and can produce 162.05 mg L −1 COR 37 . Huo et al constructed a COR‐producing S. cerevisiae strain, which was fermented and optimized to reach 137.27 mg L −1 of COR 17 .…”