Cord blood‐derived human cultured mast cells produce transforming growth factor β<sub>1</sub>

Kanbe, Naotomo; Kurosawa, Motohiro; Nagata, H; Saitoh, Hiroki

doi:10.1046/j.1365-2222.1999.00459.x

Cited by 58 publications

(43 citation statements)

References 40 publications

(78 reference statements)

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“…Therefore, our data indicate that TGF-b1 from MCs and ECs can be activated by allergen-IgE receptor-mediated stimulation of MCs. l g E / a n t i -l g E l g E / a n t i -l g E + S B T I l g E / a n t i -l g E + A n t i p a Our gene expression data confirmed the previous report by Kanbe and colleagues (35), demonstrating that human MCs constitutively express TGF-b1 and that the levels of TGF-b1 mRNA are not dramatically altered by stimulation. The real-time RT-PCR data also indicate that TGF-b1 is probably the main form of TGF-b produced by human MCs compared with TGF-b2.…”

Section: Discussionsupporting

confidence: 81%

“…Kanbe and colleagues (35) previously reported that human MCs produce TGF-b1. However, the concentration of TGF-b1 was too low to be detectable by ELISA in their experiments, and, to our knowledge, there are no other reports of the production of active TGF-b1 in human MCs other than reports of immunohistochemical staining of TGF-b1 in MCs (36).…”

Section: Ige-stimulated Mcs Produce Significant Quantities Of Active mentioning

confidence: 99%

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Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Lee

Kato

Takabayashi

et al. 2015

Am J Respir Cell Mol Biol

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Previous reports suggest that plasminogen activator inhibitor-1 (PAI-1) promotes airway remodeling and that human and mouse mast cells (MCs) are an important source of PAI-1. In the present study we investigated MC-epithelial cell (EC) interactions in the production of PAI-1. We stimulated the human MC line LAD2 with IgE-receptor cross-linking and collected the supernatants. We incubated the human bronchial EC line BEAS-2B with the LAD2 supernatants and measured the level of PAI-1. When the supernatants from IgE-stimulated LAD2 were added to BEAS-2B, there was a significant enhancement of PAI-1 production by BEAS-2B. When we treated the MC supernatants with a transforming growth factor (TGF)-b1 neutralizing antibody, the MC-derived induction of PAI-1 from BEAS-2B was completely abrogated. Although TGF-b1 mRNA was constitutively expressed in resting LAD2, it was not highly induced by IgE-mediated stimulation. Nonetheless, active TGF-b1 protein was significantly increased in LAD2 after IgE-mediated stimulation. Active TGF-b1 produced by primary cultured human MCs was significantly reduced in the presence of a chymase inhibitor, suggesting a role of MC chymase as an activator of latent TGF-b1. This study indicates that stimulation of human MCs by IgE receptor cross-linking triggers activation of TGF-b1, at least in part via chymase, which in turn induces the production of PAI-1 by bronchial ECs. Our data suggest that human MCs may play an important role in airway remodeling in asthma as a direct source of PAI-1 and by activating bronchial ECs to produce further PAI-1 via a TGF-b1-mediated activation pathway.

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Section: Discussionsupporting

confidence: 81%

Section: Ige-stimulated Mcs Produce Significant Quantities Of Active mentioning

confidence: 99%

Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Lee

Kato

Takabayashi

et al. 2015

Am J Respir Cell Mol Biol

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“…In addition, IL-4-positive mast cells are signiWcantly associated with the size of allergic skin prick-test wheal reaction and serum total IgE levels [156]. Even though human mast cells have been shown to express IL-6 and TGF-1 [88,99]; it is, however, not known to what extent mast cells contain these Th17 priming cytokines in the psoriatic skin. Our preliminary results suggest that mast cells contain IL-6 immunoreactivity in the developing psoriatic lesion induced by the tape-stripping technique (Suttle et al, unpublished results) (Fig.…”

Section: Mast Cells In Relation To Cells Of the Immune System In Psormentioning

confidence: 99%

Is there a role for mast cells in psoriasis?

et al. 2008

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Mast cells have traditionally been considered as eVector cells in allergy but during the last decade it has been realized that mast cells are essentially involved in the mechanisms of innate and acquired immunity. Upon activation by anaphylactic, piecemeal degranulation or degranulation-independent mechanisms mast cells can secrete rapidly or slowly a number of soluble mediators, such as serine proteinases, histamine, lipid-derived mediators, cytokines, chemokines and growth factors. Mast cells can express cell surface co-stimulatory receptors and ligands, and they can express MHC class II molecules and thereby present antigens. These soluble factors and cell surface molecules can interact with other cells, such as endothelial cells, keratinocytes, sensory nerves, neutrophils, T cell subsets and antigen presenting cells which are essential eVectors in the development of skin inXammation. Besides promoting inXammation, mast cells may attempt in some circumstances to suppress the inXammation and epidermal growth but the regulation between suppressive and proinXammatory mechanisms is unclear. Psoriasis is characterized by epidermal hyperplasia and chronic inXammation where tryptase-and chymase-positive MC TC mast cells are activated early in the developing lesion and later the cells increase in number in the upper dermis with concomitant expression of cytokines and TNF superfamily ligands as well as increased contacts with neuropeptide-containing sensory nerves. Due to the intimate involvement of mast cells in immunity and chronic inXammation the role of mast cells in psoriasis is discussed in this review.

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“…The mast cells produce a variety of fibrogenic cytokines, including plateletderived growth factor, transforming growth factor-b (TGF-b), and basic fibroblast growth factor. [3][4][5] These cytokines are involved in numerous inflammatory and neoplastic conditions in which marrow fibrosis is encountered and are thus not specific to mast cells. In addition, mast cell tryptase, which has been shown to be a powerful fibroblast mitogen, 6 is thought to play an important fibrogenic role in systemic mastocytosis.…”

mentioning

confidence: 99%

The stromal composition of mast cell aggregates in systemic mastocytosis

et al. 2009

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Systemic mastocytosis is a stem cell disorder characterized histologically by the presence of multifocal compact aggregates of mast cells in at least one extracutaneous organ with or without evidence of skin lesions. The mast cell aggregates are accompanied by fibrosis, which is often significant. However, in spite of its frequent occurrence and severity, little is known about its characteristics. In this study, we evaluated the composition of the fibrotic mast cell aggregates by studying eight bone marrow biopsies and two spleens involved by systemic mastocytosis, and compared the findings with those observed in other fibrotic bone marrow disorders such as primary myelofibrosis and metastatic malignancy. Histochemistry and immunohistochemistry were used to evaluate: (a) extracellular matrix (reticulin, trichrome, collagen IV, laminin); (b) stromal reticulum cells (low-affinity nerve growth factor receptor); (c) presence of myofibroblastic differentiation (smooth muscle actin) and (d) microvessel density (CD34). We found that all cases showed marked reticulin and collagen fibrosis. However, unlike primary myelofibrosis and metastatic malignancy, which are usually associated with increased low-affinity nerve growth factor receptor positivity, its expression was low in all cases of systemic mastocytosis. Myofibroblastic differentiation was only focally detected in two of eight bone marrow biopsies. In all cases, the systemic mastocytosis lesions were largely devoid of type IV collagen and laminin. The latter findings were in contrast with those seen in cases of primary myelofibrosis and metastatic malignancy where smooth muscle actin, collagen IV and laminin were expressed in most cases. Also in contrast with the other two conditions, only minimal vascularity was detectable within the fibrotic mast cell lesions. These findings indicate that systemic mastocytosis exhibits a distinct pattern of stromal change, and suggest that the fibrogenetic mechanism in systemic mastocytosis is most likely different from that of other bone marrow neoplasms which are also associated with fibrosis.

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Cord blood‐derived human cultured mast cells produce transforming growth factor β₁

Abstract: Cord blood-derived human cultured mast cells constitutively express mRNA for TGF-beta1 and produce functional TGF-beta1. Because TGF-beta1 has been shown to be highly fibrogenic, these results may highlight a novel role for human mast cells in tissue fibrosis.

Cited by 58 publications

References 40 publications

Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Is there a role for mast cells in psoriasis?

The stromal composition of mast cell aggregates in systemic mastocytosis

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Cord blood‐derived human cultured mast cells produce transforming growth factor β1

Abstract: Cord blood-derived human cultured mast cells constitutively express mRNA for TGF-beta1 and produce functional TGF-beta1. Because TGF-beta1 has been shown to be highly fibrogenic, these results may highlight a novel role for human mast cells in tissue fibrosis.

Cited by 58 publications

References 40 publications

Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Cross-Talk between Human Mast Cells and Bronchial Epithelial Cells in Plasminogen Activator Inhibitor-1 Production via Transforming Growth Factor-β1

Is there a role for mast cells in psoriasis?

The stromal composition of mast cell aggregates in systemic mastocytosis

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Cord blood‐derived human cultured mast cells produce transforming growth factor β₁