Copolymeric hydrogel membranes for immobilization and cultivation of human stem cells

Abstract

The In sti tute of Biocolloid Chem is try named af ter F.D. Ovcharenko, NAS of Ukraine 42, Ac a de mi cian Vernadsky blvd., Kyiv, 03142, Ukraine 1 The In sti tute of Mo lec u lar Bi ol ogy and Ge net ics, NAS of Ukraine 150, Ac a de mi cian Zabolotny Str., Kyiv, 03143, Ukraine Sig nif i cant amount of hydrogel mem branes for stem cells cul ti va tion has been syn the sized on the ba sis of acrylamide, acrylonitrile, and acrylic acid by the method of rad i cal copolymerization. The com par a tive in ves ti ga tions have shown that the op ti mal pa ram e ters, for the im mo bi li za tion of hu man mesenchymal stem cells are in her ent to nonionic co pol y mer hydrogels on acrylamide and acrylonitrile bases. The use of hydrogels with con sid er able acrylic acid con tent is lim ited by pH de crease in cul ti va tion me dium, which is in com pat i ble with life ac tiv ity of the cells un der in ves ti ga tion.

Polyacrylamide Hydrogel Enriched with Amber for In Vitro Plant Rooting

“…Such hydrogels are characterized by high hydrophilicity and biological tolerance, as well as improved optical, sorption, and diffusion properties, which can be adjusted by varying the monomer composition and cross-linking density. The listed peculiarities make them suitable for immobilization of a wide range of both organic and inorganic compounds, as well as for use in novel technologies, namely water treatment [17], manufacturing of selective membranes [18,19], cell cultivation (in particular, stem cells) [19], targeted delivery, and controlled release of anticancer drugs [15]. In recent years, substrates based on such hydrogels have found application for plant vegetation under controlled conditions due to their ability to sorb and slowly release the necessary bioelements into the environment under plant root exudate action [20][21][22][23].…”

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

“…Starting from 2006 until nowadays, on the basis of experimental research data the world's scientists came to the conclusion that mesenchymal stem cells (MSCs) or multipotent mesenchymal stromal cells (MMSCs) and their derivatives, exosomes and secretomes (cultural mediums conditioned by MSCs), are really promising biologically active components for biotechnological wound coverings [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Taking this into account, it was inte res ting for us to compare the activity of human cells of different types and origin in the same experiments.…”

“…In our works [1-4, 12-16, 19-21] we are talking about the creation of new biotechnological dermal coatings: the features of the materials selection for the cellular carrier, as well as cellular components, cells and/or their derivatives, in comparison with known literature data. Polyacrylamide hydrogel was used by us as a first sample of cell carrier for temporary dermal equivalents [1][2][3][4]12]. At the beginning of experimental research in the field of cellular technologies, polyacrylamide hydrogels were not used for the manufacturing of artificial wound coverings.…”

Cross-Linked Hydrogels Based on PolyNIPAAm and Acid-Activated Laponite RD: Swelling and Tunable Thermosensitivity

“…Hydrogels are the hydrophilic three-dimensional polymeric networks that can absorb large amounts of water or other biological fluids, and they are excellent matrixes for drug delivery, growing and cultivating of cells, water purification, and creation of selective membranes . The pioneering studies of Haraguchi made it possible to achieve a significant improvement in the mechanical characteristics of polymer hydrogels by physical cross-linking using synthetic hectorite clay Laponite (Lap) nanoparticles (produced by BYK Additives Ltd.) instead of traditional chemical cross-linking agentsbifunctional monomers (e.g., N , N ′-methylene-bis-acrylamide).…”