Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers

Dußle, C. M.; Quint, Marcel; Xu, Ming; Melchinger, Albrecht E.; Lübberstedt, T.

doi:10.1007/s00122-002-0964-7

Cited by 29 publications

(12 citation statements)

References 28 publications

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“…Yuan et al (2003) using a different maize population consisting of 121 F 3 lines from cross F7 (susceptible)×FAP1360 (resistant), found that the Scmv1 region harbored two QTLs rather than one as identified in previous studies. Dussle et al (2002) mentioned that in different studies a reason for the lack of resistance‐allele‐specific co‐segregating markers could be the presence of more than one SCMV resistance gene in the Scmv1 region. However, we found clustered QTLs ( Scm2a and Scm2b ) for SCMV resistance at chromosome 3 (bin 3.04).…”

Section: Discussionmentioning

confidence: 99%

Mapping QTL contributing to SCMV resistance in tropical maize

et al. 2008

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Sugarcane mosaic virus (SCMV) has been increasing in importance as a maize disease in Brazil. In this study, we mapped and characterized quantitative trait loci (QTL) associated with resistance to SCMV in a maize population consisting of 150 F 2:3 families from the cross between two tropical maize inbred lines, L520 (resistant) and L19 (susceptible). F 2 individuals were genotyped with microsatellite (SSR) markers, and the derived F 2:3 families were evaluated for their response to artificial inoculation with SCMV under field conditions at Sete Lagoas, MG, Brazil, in 2001 and 2005. Multiple interval mapping was used for QTL detection with a linkage map based on 19 SSR markers. Three QTLs for SCMV resistance were identified with two QTLs (Scm2a and Scm2b) clustered on chromosome 3, bin 3.04, and one QTL (Scm1) on chromosome 6, bin 6.01, explaining 13.34, 41.85 and 7.66% of the phenotypic variation for SCMV resistance, respectively.

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Section: Discussionmentioning

confidence: 99%

Mapping QTL contributing to SCMV resistance in tropical maize

et al. 2008

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“…AFLP has been effectively used to assess genetic diversity (Hagen et al 2002;Le Clerc et al 2002;Lerma et al 2003), construct high density genetic maps (Katengam et al 2002;Strommer et al 2002), analyse quantitative traits (Hoffman and Dahleen 2002;Salland et al 2003), and to enrich DNA markers near a locus of interest (Dussle et al 2002;Duble et al 2003). In sugarcane, attempts have been made to map QTLs for yield components (Hoarau et al 2002), tag genes for smut resistance, establish close linkage with rust resistance gene (Hoarau et al 2001), and to assess genetic diversity among the Brazilian cultivars (Lima et al 2002) and in the related genera Erianthus ) using AFLP markers.…”

Section: Introductionmentioning

confidence: 99%

AFLP Analysis of the Phenetic Organization and Genetic Diversity in the Sugarcane Complex, Saccharum and Erianthus

Selvi

Nair

Noyer

et al. 2006

Genet Resour Crop Evol

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Amplified fragment length polymorphism (AFLP) markers were evaluated for determining the phylogenetic relationships, and the diversity in the Saccharum complex using 30 clones belonging to S. officinarum, S. robustum, S. spontaneum, S. barberi, S. sinense and the related genus Erianthus. The phenetic tree of the species clones based on AFLP data was consistent with the known taxonomical relationships. AFLP gave higher resolution of closely related species into discrete groups than that by RAPD and RFLP markers, reported earlier. The levels of diversity within the various Saccharum species were also found to be higher than those obtained previously with the same set of clones using RAPD markers. The intraspecies similarity in S. barberi and S. sinense was much higher than interspecies similarity suggesting a clear separation of the two, which are considered 'horticultural species'. The genetic similarity matrix derived from a single primer combination highly correlated (r = 0.980) with that obtained from all the 12 primer combination used in the study, thus highlighting the efficiency of a single primer combination in delineating species relationships. All the primer combinations could identify markers that are specific to each of the species and the genus Erianthus. Among the species, specific markers were highest in S. spontaneum followed by S. robustum, S. barberi, S. officinarum and S. sinense. Erianthus had a distinct profile with 30% of the total amplified fragments being specific to it. This offers great scope for identifying intergeneric hybrids, which has been very difficult using morphological traits and RAPD markers. High degree of correspondence between the results from the cluster analysis based on Jaccard's similarity index, Neighbour Joining tree based on Sokal and Michener distance matrix and AFTD (Analyses Factorielle on Table of Distances) analysis clearly demonstrated that AFLP markers would be an appropriate tool in providing better information about the relationships among the species, estimation of diversity, and in revealing species and genus specific markers that could be directly applied in sugarcane breeding programmes.

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“…Non-persistently transmission of SCMV by aphids makes the control of the virus vectors rather inefficient. Therefore, cultivation of resistant varieties is the most promising approach for control of SCMV (Dussle et al, 2002) which in turn requires a complete understanding of the genetic diversity of the virus. Several studies have been performed in recent decades on SCMV biology, genome characterization and sequence diversity (Achon et al, 2007; Alegria et al, 2003; Gao et al, 2011; Wang et al, 2010).…”

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confidence: 99%

Occurrence and Evolutionary Analysis of Coat Protein Gene Sequences of Iranian Isolates of Sugarcane mosaic virus

Moradi

Nazifi

Mehrvar

2017

The Plant Pathology Journal

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Sugarcane mosaic virus (SCMV) is one of the most damaging viruses infecting sugarcane, maize and some other graminaceous species around the world. To investigate the genetic diversity of SCMV in Iran, the coat protein (CP) gene sequences of 23 SCMV isolates from different hosts were determined. The nucleotide sequence identity among Iranian isolates was more than 96%. They shared nucleotide identities of 75.5–99.9% with those of other SCMV isolates available in GenBank, the highest with the Egyptian isolate EGY7-1 (97.5–99.9%). The results of phylogenetic analysis suggested five divergent evolutionary lineages that did not completely reflect the geographical origin or host plant of the isolates. Population genetic analysis revealed greater between-group than within-group evolutionary divergence values, further supporting the results of the phylogenetic analysis. Our results indicated that natural selection might have contributed to the evolution of isolates belonging to the five identified SCMV groups, with infrequent genetic exchanges occurring between them. Phylogenetic analyses and the estimation of genetic distance indicated that Iranian isolates have low genetic diversity. No recombination was found in the CP cistron of Iranian isolates and the CP gene was under negative selection. These findings provide a comprehensive analysis of the population structure and driving forces for the evolution of SCMV with implications for global exchange of sugarcane germplasm. Gene flow, selection and somehow homologous recombination were found to be the important evolutionary factors shaping the genetic structure of SCMV populations.

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Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers

Cited by 29 publications

References 28 publications

Mapping QTL contributing to SCMV resistance in tropical maize

Mapping QTL contributing to SCMV resistance in tropical maize

AFLP Analysis of the Phenetic Organization and Genetic Diversity in the Sugarcane Complex, Saccharum and Erianthus

Occurrence and Evolutionary Analysis of Coat Protein Gene Sequences of Iranian Isolates of Sugarcane mosaic virus

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