Mapping QTL contributing to SCMV resistance in tropical maize

Abstract

Sugarcane mosaic virus (SCMV) has been increasing in importance as a maize disease in Brazil. In this study, we mapped and characterized quantitative trait loci (QTL) associated with resistance to SCMV in a maize population consisting of 150 F 2:3 families from the cross between two tropical maize inbred lines, L520 (resistant) and L19 (susceptible). F 2 individuals were genotyped with microsatellite (SSR) markers, and the derived F 2:3 families were evaluated for their response to artificial inoculation with SCMV under field conditions at Sete Lagoas, MG, Brazil, in 2001 and 2005. Multiple interval mapping was used for QTL detection with a linkage map based on 19 SSR markers. Three QTLs for SCMV resistance were identified with two QTLs (Scm2a and Scm2b) clustered on chromosome 3, bin 3.04, and one QTL (Scm1) on chromosome 6, bin 6.01, explaining 13.34, 41.85 and 7.66% of the phenotypic variation for SCMV resistance, respectively.

Combined linkage and association mapping reveals candidates for Scmv1, a major locus involved in resistance to sugarcane mosaic virus (SCMV) in maize

“…High-resolution mapping using progeny from the cross between FAP1360A (completely resistant to SCMV) and F7 (highly susceptible to SCMV) confirmed that Scmv1 (on the short arm of chromosome 6) and Scmv2 (near the centromere of chromosome 3) are crucial for maize resistance to SCMV [ 13 ]. Similar mapping results have been reported in several independent experiments [ 8 , 14 , 15 ]. The Scmv1 locus has also been detected in a resistant Chinese line, Huangzao4, together with additional QTL on chromosomes 1, 3, 5, and 10 [ 14 ].…”

“…By using an F 9 -derived immortalized RIL population obtained from the cross of Huangzao4×Mo17, a major QTL was detected between chromosome bins 6.00 and 6.01, accounting for 50.0% of the total phenotypic variation and decreasing the disease index by 27.9% [16]. The loci on chromosomes 3 and 6 have also been confirmed in tropical maize germplasm from Brazil [15]. With the aid of the whole-genome B73 sequence, the Scmv2 region was fine-mapped to a region of 0.28 cM, covering a physical distance of 1,342.6 kb.…”

Screening of NCLB Disease and QTLs Mapping in Zea mays

“…Gene action was mostly partially dominant or recessive. This result is accordance to Souza [25] who observed over dominance gene action for mostly region for SCMV disease in maize.…”

Sugarcane Mosaic Disease: Characteristics, Identification and Control

“…The development of molecular markers associated with economic target traits is an extremely slow process for sugarcane. Previous studies on the development of molecular markers for mosaic resistance were mainly focus on corn (2 n = 2 x = 20; genome size =~2300 Mb) [156][157][158][159][160][161][162][163][164]. However, these studies may provide a good reference for developing molecular markers and related gene mining on sugarcane.…”