Conventional PCR for detection of Corynespora cassiicola in soybean seeds

Sousa, Marcella Viana de; Siqueira, Carolina da Silva; Machado, José da Cruz

doi:10.1590/2317-1545v38n2152049

Cited by 8 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The vast majority relies on the seed suppliers to provide "clean" seed often with no interest in their seed borne disease status. Due to economics and current environmental awareness, more research effort needs to be directed toward biological seed treatments with further development and application of advanced DNA amplification technologies to enhance the detection of seedborne pathogens that otherwise will go undetected using conventional seed health testing methods (Sousa et al, 2015(Sousa et al, , 2016.…”

Section: Seed Qualitymentioning

confidence: 99%

Enhancing Seed Health for Organic Vegetable Production Systems: Challenges and Opportunities

Nyochembeng¹

2021

JAS

View full text Add to dashboard Cite

Organic vegetable production is a rapidly expanding segment within the fast growing organic sector of agricultural production. Although pests and diseases remain a challenge in organic production, the growth and expansion of this system is dependent on sustained use of good quality organic seed. Due to the limited supply of organic seed, the National Organic Program (NOP) allows the use of untreated conventional seed in organic production of vegetables and other crops. Conventional seed derives from a high input production system using synthetic pesticides and fertilizers. They also offer many varieties and are readily available at a much lower price compared to organic seed. Organic systems demand cultivars with different characteristics often absent in conventional cultivars, and this need begins with the seed. It is not common practice for farmers to test or sanitize seed before planting. Consequently, the use of such conventional seeds, which may not be well adapted to the low input organic production system, could favor disease susceptibility, establishment of seedborne pathogens especially in vegetables and their subsequent dissemination in the organic production system. Our overall goal is to improve organic vegetable crop health and production in the southeastern U.S. through application of sustainable seed health management and help limit seed borne infections, transmission and dissemination in organic vegetable production fields.

show abstract

Section: Seed Qualitymentioning

confidence: 99%

Enhancing Seed Health for Organic Vegetable Production Systems: Challenges and Opportunities

Nyochembeng¹

2021

JAS

View full text Add to dashboard Cite

show abstract

“…Detection of phytopathogens in seeds has become increasingly important especially because of the relevance of this measure as part of plant health management (SOUSA; SIQUEIRA; MACHADO, 2016;SAJEESH et al;2014).…”

Section: Introductionmentioning

confidence: 99%

Corynespora cassiicola in soybean seeds – incidence and transmission

Goulart

Utiamada²

2020

Biosci. J.

View full text Add to dashboard Cite

The fungus Corynespora cassiicola, causal agent of target spot in soybeans, has been considered, from the seed pathology point of view, a seed-borne pathogen of limited importance. Therefore, little importance has been given to the role of the seeds in the transmission of this pathogen. The objectives of this study were to determine the incidence of C. cassiicola in soybean seeds and evaluate the effects of this seed-borne pathogen, inoculated in the seeds, in relation to physiological and epidemiological parameters. The experiments were carried out at TAGRO and Embrapa Western Agriculture under lab (blotter test and seed germination test) and greenhouse conditions (growing on test). The fungus C. cassiicola was detected in 11.3% of the 639 seed samples analyzed, with an average incidence of 0.91% and maximum of 8.5%. The transmission of C. cassiicola from the seeds to above-ground parts of soybean seedlings was demonstrated, by pathogen establishment on the cotyledon, showing circular lesions with concentric rings, reddish-brown in the center and surrounded by a yellowish green halo, as a typical symptoms of target spot. Reddish-brown lesions on the roots and stem of the seedlings were also observed. Considering a sample seed with 66.0% of C. cassiicola incidence, the symptomatic transmission based on cotyledon symptoms was 42.2%, corresponding to a transmission rate of 2.4:1. This is the first report, in a quantified way, about the transmission of C. cassiicola from the seeds to above-ground parts of soybean seedlings. When compared to non-inoculated seeds, seed germination, seedling emergence and seedling initial development were influenced by the presence of the pathogen in the seeds, with the lowest values being observed when the seeds were inoculated.

show abstract

“…This technology was successful in detecting, for example, Stenocarpella complex (S. maydis and S. macrospora) in maize (Romero and Wise, 2015), Fusarium oxysporum f.sp. phaseoli in bean seeds (Sousa et al, 2015), Sclerotinia sclerotiorum in soybean seeds (Botelho et al, 2015) and Corynespora cassiicola in soybean seeds (Sousa et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Specificity and sensibility of primer pair in the detection of Colletotrichum gossypii var. cephalosporioides in cotton seeds by PCR technique

et al. 2020

Self Cite

View full text Add to dashboard Cite

Cotton Ramulosis (Gossypium hirsutum) is an important disease affecting cotton plantations in Brazil, and its causal agent, Colletotrichum gossypiivar.cephalosporioides(Cgc), according to the Brazilian phytosanitary authority, was considered a regulated non quarantine pest. It makes this microorganism subject to standardization in seed certification programs. The current seed health testing for detecting that pathogen in seed samples does not provide reliable results for routine analysis. On this paper, attempts were made to design specific primers for detection of Cgc associated with cotton seed. Two primer sets were selected based on the analysis of a multiple alignment of gene’s sequence encoding the glyceraldehyde 3-phosphate dehydrogenase from Cgc, C. gossypii and reference strains of the C. gloeosporioides species complex. The conserved sites unique to Cgc strains were used to design specific fragment of 140 bp. The primer specificity was confirmed by using other fungi. The primers produced a detectable band of target DNA of Cgc in all inoculum potentials of the pathogen artificially inoculated by the water restriction technique. The developed primer pair represents, therefore, a reliable and rapid mean to diagnose the Ramulosis agent in cotton seed.

show abstract

Conventional PCR for detection of Corynespora cassiicola in soybean seeds

Cited by 8 publications

References 16 publications

Enhancing Seed Health for Organic Vegetable Production Systems: Challenges and Opportunities

Enhancing Seed Health for Organic Vegetable Production Systems: Challenges and Opportunities

Corynespora cassiicola in soybean seeds – incidence and transmission

Specificity and sensibility of primer pair in the detection of Colletotrichum gossypii var. cephalosporioides in cotton seeds by PCR technique

Contact Info

Product

Resources

About