Conventional high-performance liquid chromatography versus derivative spectrophotometry for the determination of 1,3,6-pyrenetrisulfonic acid trisodium salt and 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt in the color additive D&amp;C Green No. 8 (Pyranine)

Jitian, Simion; White, Samuel; Yang, H H Wendy; Weisz, Adrian

doi:10.1016/j.chroma.2013.11.033

Cited by 4 publications

(1 citation statement)

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“…By setting the flow rate and displaying the detection time during cell counting, FCM is convenient and time-saving compared with traditional plate counting [16,17]. In addition, measurement of dyes has an important application in techniques like liquid/gas chromatography and mass spectrometry [18,19]. However, the application of labeling dyes is not productive if the dyes are of unknown composition or if their adsorption/emission spectra have significant overlap.…”

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confidence: 99%

Optimization of Staining with SYTO 9/Propidium Iodide: Interplay, Kinetics and Impact on Brevibacillus Brevis

et al. 2020

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Fluorophores SYTO 9 and propidium iodide (PI) are extensively applied in medicine, food industry and environmental monitoring to assess the viability of bacteria. However, the actual performance of these dyes remains largely unknown. In addition, their effects on the physiology of cells have not been elucidated. Here we characterized the effects of these two dyes on Brevibacillus brevis under optimized staining. We found that SYTO 9 entered cells continuously while PI tended to adhere to the cell wall before entering the cell. In addition, results showed that a high amount of the dyes altered the physicochemical properties of membranes, improving their breakthrough. These results provide new perspectives and ideas for improving the characterization of bacterial viability using flow cytometry.

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confidence: 99%