Controlled release of FGF-2 and BMP-2 in tissue engineered periosteum promotes bone repair in rats

Abstract

The aim of this study was to prepare chitosan-collagen (CS/COL) scaffolds that could release fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein 2 (BMP-2), and to study the effect of this scaffold on bone repair. By improving the double emulsion/solvent evaporation technique, BMP-2 was encapsulated in poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PELA) microcapsules, to the surface of which FGF-2 was attached. The CS/COL scaffold carrying the microcapsules was prepared by freeze-drying. Periosteum derived cells (PDCs) were extracted and cultured on the scaffolds to study their proliferation and differentiation on the scaffolds. In addition, the effects of the scaffolds were investigated on rats with skull defects by micro-computed tomography and histology. We successfully prepared PELA microcapsules with external adherence to FGF-2 and encapsulated with BMP-2. The CS/COL scaffolds were porous and PDCs adhered, proliferated and underwent osteogenic differentiation on the scaffolds. The sequential release of FGF-2/BMP-2 had better osteogenic efficacy than other groups. Our results suggest that CS/COL scaffolds that bind FGF-2 and BMP-2 in combination with PDCs could be a promising new strategy for tissue engineering periosteum.

Acceleration of Pelvic Tissue Construction by Overexpression of Basic Fibroblast Growth Factor in the Stem Cells

“…In this study have shown that indirect co-culture can promote the synthesis of type and collagen bers by ADSC bFGF , and successfully induce themselves differentiate into broblasts [29][30]. Lee et al [31] added connective tissue growth factor to the culture medium to differentiate bone marrow umbilical cord mesenchymal stem cells into broblasts. Yin et al [32] colonized mesenchymal stem cells on interlaced scaffold materials.…”

“…It is one of the important wound healing factors in the body, has a wide range of biological activities and can affect early in ammation, granulation and the entire wound repair process such as tissue regeneration, epithelialization, and tissue remodeling,thus, has an important application value [13].Our data indicate successfully induced the ADSCs into broblasts by bFGF in vitro, and con rmed that bFGF can signi cantly increase the proliferation of differentiated broblasts, moreover the expression of collagen.However, the growth factor half-life is short and the biological permeability is poor, so it is very easy to be inactivated, and repeated dosing is required to maintain the local concentration [13]. Additionally, studies have shown that bFGF injection is associated with severe anemia, which limits the systemic application of bFGF [14][15]. Therefore, it is necessary to achieve continuous release and an effective concentration of bFGF, in order to provide a suitable microenvironment for the directed differentiation of stem cells.…”

Scleral ossicles: angiogenic scaffolds, a novel biomaterial for regenerative medicine applications

“…[27][28][29][30][31][32] A variety of different exogenous chemical or physical stimuli were tested, such as soluble growth/differentiation factors, as well as the application of mechanical forces (i.e., loading). [33][34][35][36][37][38][39][40] Finally, many types of cells have been used alone or in coculture. [41][42][43][44][45][46][47][48] An engineered scaffold-based strategy is the starting point of the recent innovative therapies, for which the choice of material properties, manufacturing methods and component treatments, is crucial.…”

Construction and characterization of an adenoviral vector encoding human bone morphogenetic protein-2

“…10,11 Our work makes high level gene expression of BMP-2 possible and lays the foundation for future applications of BMP-2 in gene therapies for orthopedic conditions including bone defects 12,13 as well as in bone tissue engineering. 14,15 Material and methods…”