Controle De Qualidade Analítica Dos Métodos Utilizados Para a Detecção De Protozoários Patogênicos Em Amostras De Água

Neto, Romeu Cantúsio; Santos, Luciana Urbano dos; Sato, Maria Inês Z.; Franco, Regina Maura Bueno

doi:10.1590/1808-1657v78p1692011

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…Even though the collections were carried out in the urban area, this nding is of public health importance as it demonstrates that the lack of prevention of parasites in the rural area can lead to public health problems in the urban area. The presence of protozoa, in greater numbers Giardia, may be linked to environments with precarious basic sanitation, as cysts are released in the feces and can contaminate water and vegetables, one of the main forms of dissemination of the disease, in addition to the lack of hygiene after contact direct from the hands with objects contaminated by the cysts [41]. Thus, the municipalities of Canguçu, Morro Redondo and Turuçu need to invest in public policies that help the population in changing habits and hygiene practices, which contribute to minimizing the risks of the disease.…”

Section: Resultsmentioning

confidence: 99%

Risk analysis of contamination by potentially zoonotic parasites found in public squares at Southern Brazil

Lignon

Bohm

Martins

et al. 2023

Preprint

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Background Human beings and animals are getting closer and closer. This proximity is not limited to the home environment, as animals are also frequenters of public spaces with access to leisure areas and human recreation. As they play the role of definitive hosts for some species of potentially zoonotic helminths and protozoa, the risk of environmental contamination through their feces and the risk of infection of other animals, including humans, increases. From an epidemiological point of view, identifying possible sites at risk of infection allows us to outline adequate control and prevention methods. Therefore, the aim of this work was to carry out a risk analysis of contamination by potentially zoonotic parasites found in public squares in Southern Brazil. Methods Samples of dog feces and sand were collected in public squares in municipalities in the Pelotas microregion, south of Rio Grande do Sul, Brazil. The parasitological diagnosis was performed using the techniques of Willis-Mollay, Faust and Spontaneous sedimentation for feces and Caldwell and Caldwell for sand. The results were interpreted through descriptive data analysis, chi-square test and correspondence analysis. Results In total, 4262 samples were analyzed, where 63% of sand samples and 49.8% of feces samples were parasitized by Ancylostoma. Of all the municipalities in the study, Cerrito, Capão do Leão and Cristal have the highest risk for the presence of parasites in the samples collected. In addition, Pelotas and Pedro Osório municipalities had more positive samples with nematode eggs; Cristal, Cerrito and Capão do Leão had more samples positive for tapeworms; Canguçu, Morro Redondo and Turuçu showed higher positivity of protozoa. Conclusion It is concluded that there is great contamination in the squares studied, by parasites with zoonotic potential, and among the identified parasites, Ancylostoma is the most frequent. Furthermore, it is suggested that small municipalities are at greater risk of presenting parasites in public spaces, such as squares.

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Section: Resultsmentioning

confidence: 99%

Risk analysis of contamination by potentially zoonotic parasites found in public squares at Southern Brazil

Lignon

Bohm

Martins

et al. 2023

Preprint

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Occurrence of Cryptosporidium spp., Giardia spp. and other pathogenic intestinal parasites in the Beberibe River in the State of Pernambuco, Brazil

Freitas

Paiva

Filho

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction: Transmission of pathogenic protozoa and helminths by water is a serious public health problem. In this study, we analyzed the presence of these organisms in the Beberibe River in Pernambuco, Brazil. Methods: Parasite analysis was performed using the Hoffman, Pons, & Janer method followed by centrifugation and preparation of slides by staining with acetic acid and Lugol's solution. Protozoan oocysts were isolated by the modifi ed Ziehl Neelsen method. Results: Cryptosporidium spp., Giardia spp. and other parasites were found in the Beberibe River. Conclusions: Sanitation companies must assess pathogenic intestinal parasites in water basins providing public water and subsequently develop improved treatment systems for removal of such parasites.

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Immunomagnetic separation study applied to detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in water samples

Pinto¹,

Urbano

Neto³

2015

Water Supply

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In this work we studied the IMS purification comparing acid (n = 12) and heat (n = 12) dissociation procedures and investigated the possible losses of target organism in this step. Reagent water samples were directly inoculated with Giardia spp. cysts and Cryptosporidium spp. oocysts (BTF Easy Seed™). Acid dissociation showed higher mean recovery efficiency and precision than heat dissociation for Giardia spp. cysts and Cryptosporidium spp. oocysts, but there were not significant statistic differences for Cryptosporidium spp. oocysts. Mean recovery efficiency for both protozoa were in accordance with the acceptance criteria of Method 1623 by acid and heat dissociation. The bead–cyst/bead–oocysts dissociation procedure is fundamental for better results whereas a significant loss of organisms occurs in this step.

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Controle De Qualidade Analítica Dos Métodos Utilizados Para a Detecção De Protozoários Patogênicos Em Amostras De Água

Cited by 5 publications

References 14 publications

Risk analysis of contamination by potentially zoonotic parasites found in public squares at Southern Brazil

Risk analysis of contamination by potentially zoonotic parasites found in public squares at Southern Brazil

Occurrence of Cryptosporidium spp., Giardia spp. and other pathogenic intestinal parasites in the Beberibe River in the State of Pernambuco, Brazil

Immunomagnetic separation study applied to detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in water samples

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