Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions

Makridis, Pavlos; Fjellheim, Anders Jón; Skjermo, Jorunn; Vadstein, Ôlav

doi:10.1016/s0044-8486(99)00351-8

Cited by 85 publications

(70 citation statements)

References 28 publications

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“…Both rotifers and Artemia sp. are filter feeders capable of concentrating large amounts of bacteria, and live feed is a major source of bacterial influx to the gastrointestinal tract of fish (Nicolas et al 1989, Skjermo & Vadstein 1993, Makridis et al 2000a. The present results support the view that the composition of this influx influences larval survival.…”

Section: Discussionsupporting

confidence: 83%

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Sandlund

Bergh²

2008

Dis. Aquat. Org.

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In intensive aquaculture systems, high concentrations of nutrients and high densities of fish larvae provide favorable conditions for opportunistic pathogenic bacteria to flourish. We screened potentially pathogenic bacterial strains isolated from moribund Atlantic cod Gadus morhua larvae, pollack Pollachius pollachius, coalfish Pollachius virens, Atlantic halibut Hippoglossus hippoglossus, rotifers, algae and water samples from different hatcheries. Three identical challenge experiments tested a total of 53 strains. A multidish system was used: cod eggs were placed in single wells, together with 2 ml of sterile seawater, and exposed to the bacterial cultures. Final bacterial concentrations in the wells were 10 6 and 10 4 CFU ml -1. Eggs and larvae not exposed to bacteria were used as unchallenged controls. Challenged controls were exposed to Vibrio anguillarum strain 610. Eggs were challenged approximately 48 h prior to hatching and mortality was recorded daily throughout the yolk-sac period. In spite of the high challenge dose of 10 6 CFU ml -1, only 5 bacterial strains tested caused higher mortality than the unchallenged controls. Four of these strains were identified by 16S rDNA and gyrase B gene (GyrB) sequencing as resembling V. anguillarum and 1 strain resembled Carnobacterium sp. Most of the larvae exposed to these strains died within 10 d of challenge. Serotyping of the strains resembling V. anguillarum gave inconclusive results. This indicates differences in serology compared to the serotypes O1, O2 and O3, associated with disease. Three bacterial strains seemed to have a slower infection rate, indicating a longer incubation period. The remaining 45 strains did not seem to have a negative effect on larval survival, suggesting that these are not primary pathogens.

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Section: Discussionsupporting

confidence: 83%

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Sandlund

Bergh²

2008

Dis. Aquat. Org.

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“…In some studies, alternative methods (eggs disinfection, use of microbially-matured water or baths of prey in tap water) to antibiotics have been considered (Vadstein et al, 1993;Planas and Cunha, 1999;Skjermo and Vadstein, 1999) in order to reduce the microflora which accompanies the prey, but also the delivery of probiotic bacteria (Gatesoupe, 1991;;Gatesoupe, 1997;Harzevili et al, 1998;Gómez-Gil et al, 1998;Makridis et al, 2000;Gatesoupe, 2002).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of rotifer (Brachionus plicatilis) growth by using terrestrial lactic acid bacteria

et al. 2004

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One of the pathways for the entry of bacteria, both pathogenic and probiotic, into the larvae of fish hatcheries is via live prey. As a preventive measure against infections, live prey may be cultured, supplementing the food with probiotics. Some lactic acid bacteria (LAB) have been successfully used in the larviculture. In this study, the nutritional effect of seven terrestrial LAB has been studied regarding the growth of the rotifer Brachionus plicatilis. The cultures were carried out without partial renewal of the culture medium, feeding the rotifers on baker's yeast and adding some of the species of bacteria. In all cases, the addition of the bacteria increased both the specific maximum growth rate and the maximum density obtainable in the cultures. However, the best results were obtained with the addition of Lactococcus lactis spp lactis, Pediococcus acidilactici or Lactobacillus casei ssp. casei. The rates of growth obtained with the individual or joint addition of these three bacteria were 8-13 times greater than those obtained with the control cultures after 4-5 days' culture. In this study, a series of kinetic models have been applied (logistic modified−Gompertz, logistic−logistic and generalised logistic) which describe the experimental data, obtaining a set of parameters of biological significance which facilitate the optimisation of the use of these bacterial strains in the mass production of rotifers.

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“…Mortality of fish larvae fed with rotifers has occurred in the past and has been related to infectious diseases. Live prey such as rotifers are important carriers of contamination of the larval digestive tract, which greatly determines the microbiota of the larvae, and in many cases, is responsible for significant mortality rates (Makridis et al, 2000). Transfer of pathogens from live prey to target organisms can be performed directly, by ingestion of the prey, and/or indirectly, by release of pathogens from prey to seawater (Prol-García et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Treatment of seawater for rotifer culture uses applying adsorption and advanced oxidation processes

Poblete-Chávez

Cortés-Pizarro

Rojas-Araya

2016

lajar

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ABSTRACT. The use of live feed is common in aquaculture processes due to its nutritional advantages, and rotifers comprise one of the most used feeds in finfish aquaculture. However, bacteria present in water used for rotifer culture are a significant source of infection and consequently diseases could cause mortality in fishes. Advanced oxidation process (AOPs) are methods that may be useful to eliminate bacterial infections present in aquaculture wastewater and may allow the reuse of this water. Therefore, the prospect of using different advanced oxidation processes to inactivate Vibrio spp. and total bacteria cultures (TCB) present in this kind of water was evaluated. In particular, UVc/O3/H2O2, UVc/H2O2 and O3/H2O2 and the adsorption process were applied to seawater coming from a fish farming tank. The results obtained were highly promising because it was possible to inactivate 100% of bacteria present in the seawater when UVc/ozone/hydrogen peroxide and UVc/hydrogen peroxide were applied, needing around 20 and 80 min for Vibrio spp. and total culturable bacteria inactivation, respectively. These results are justified due to the strong action between H2O2 in the presence of UVc, which may produce a large amount of hydroxyl radicals. Nevertheless, when ozone/hydrogen peroxide was applied, total inactivation of bacteria did not occur. Also, an interesting relationship between bacteria inactivation and oxide reduction potential of seawater was observed during the process. Rotifers put in water after the treatment did not suffer behavior changes (swimming) and no mortalities were observed. Keywords: rotifers, bacteria, UV irradiation, ozone, hydrogen peroxide, aquaculture.Tratamiento de agua de mar para el cultivo de rotíferos mediante procesos de adsorción y oxidación avanzados RESUMEN. El uso de organismos vivos como alimento es común en la acuicultura, debido a sus ventajas nutricionales. Los rotíferos constituyen uno de los alimentos principales en el cultivo de peces. Sin embargo, las bacterias presentes en aguas utilizadas en el cultivo de rotíferos son una fuente importante de infección y pueden causar enfermedades y mortalidad en peces. Los procesos de oxidación avanzados son métodos útiles para eliminar bacterias presentes en aguas residuales de la acuicultura, permitiendo su reutilización. Se evaluó la inactivación de Vibrio spp. y bacterias cultivables totales presentes en aguas residuales de la acuicultura a través procesos de oxidación avanzados. Particularmente, se estudió la aplicación de UVc/O3/H2O2, UVc/H2O2 y O3/H2O2 junto a procesos de adsorción aplicados a agua proveniente de un estanque de cultivo de peces. Los resultados obtenidos son muy prometedores debido a que fue posible inactivar el 100% de bacterias presentes cuando se utilizó UVc/O3/H2O2 y UVc/H2O2, requiriendo 20 y 80 min para la inactivación de Vibrio spp. y bacte- __________________ Corresponding editor: Ricardo Prego 780Latin American Journal of Aquatic Research rias cultivables totales, respectivamente. Estos resultados se expl...

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Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions

Cited by 85 publications

References 28 publications

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Enhancement of rotifer (Brachionus plicatilis) growth by using terrestrial lactic acid bacteria

Treatment of seawater for rotifer culture uses applying adsorption and advanced oxidation processes

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