Enhancement of rotifer (Brachionus plicatilis) growth by using terrestrial lactic acid bacteria

Abstract

One of the pathways for the entry of bacteria, both pathogenic and probiotic, into the larvae of fish hatcheries is via live prey. As a preventive measure against infections, live prey may be cultured, supplementing the food with probiotics. Some lactic acid bacteria (LAB) have been successfully used in the larviculture. In this study, the nutritional effect of seven terrestrial LAB has been studied regarding the growth of the rotifer Brachionus plicatilis. The cultures were carried out without partial renewal of the culture medium, feeding the rotifers on baker's yeast and adding some of the species of bacteria. In all cases, the addition of the bacteria increased both the specific maximum growth rate and the maximum density obtainable in the cultures. However, the best results were obtained with the addition of Lactococcus lactis spp lactis, Pediococcus acidilactici or Lactobacillus casei ssp. casei. The rates of growth obtained with the individual or joint addition of these three bacteria were 8-13 times greater than those obtained with the control cultures after 4-5 days' culture. In this study, a series of kinetic models have been applied (logistic modified−Gompertz, logistic−logistic and generalised logistic) which describe the experimental data, obtaining a set of parameters of biological significance which facilitate the optimisation of the use of these bacterial strains in the mass production of rotifers.

Effect of Lactobacillus plantarum isolated from digestive tract of wild shrimp on growth and survival of white shrimp (Litopenaeus vannamei) challenged with Vibrio harveyi

“…[6], yeast [7,8] and many Gram-negative bacteria [9,10] have been reported to effectively inhibited vibrios in shrimp cultivation. In the past 20 years, there has been great interest in the application of LAB and their metabolic products as potential probiotics for aquaculture to improve growth and survival of fish [11e13], rotifer [14,15] and shrimp [4,5,16,17]. LAB has been well-known about their ability on the production of antibacterial substances including lactic acid, acetic acid, hydrogen peroxide and bacteriocin that inhibit growth of competing bacteria [18].…”

Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture

“…In a number of studies, probiotic strains such as Lactobacillus sp., terrestrial lactic acid bacteria, Alteromonas sp. or a probiotic mixture of 40 species have proven effective against pathogens and/or considerable enhanced the growth of rotifers and fish larvae (Gatesoupe 1991;Gatesoupe 1997;Douillet 2000a;Planas et al 2004). Douillet (2000b) demonstrated a consistently enhanced rotifer growth rate with the addition of an Alteromonas strain and an unidentified Gram negative strain (B3) under all feeding regimes, in comparison with control cultures inoculated with microbial communities naturally present in seawater, or with cultures that were maintained bacteria free.…”

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“…Probiotics are used as dietary supplementations in aquaculture and their role are already established in intestinal microbial balance, growth, nutrition, health status and resistance against infectious agents (Ghomi et al 2010;Dhama et al, 2011). The positive effect of probiotics depends on both the action mechanisms and the capacity of colonization, that is to say, its ability to reach, remain or reproduce in the place where the effect is required (Planas et al, 2004).…”