Control of Salmonella Newport on cherry tomato using a cocktail of lytic bacteriophages

El-Dougdoug, Noha K.; Cucić, Stevan; Abdelhamid, Ahmed G.; Brovko, Lubov; Kropinski, Andrew M.; Griffiths, Mansel W.; Anany, Hany

doi:10.1016/j.ijfoodmicro.2019.01.003

Cited by 61 publications

(38 citation statements)

References 76 publications

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“…Phages EC10C3PV, EC11B2PV, and EC366VPV revealed the fastest growth rates; phage EC3A1PV showed the slowest growth rate from 10 to 60 min. These results are similar to those reported in previous studies (28).…”

Section: Discussionsupporting

confidence: 93%

“…The data generated were used to determine the latent period, burst time, and phage relative burst size per infected cell. The burst size was calculated as the ratio of the final count of released phage progeny to the initial count of infected bacterial host cell during the latent period using the following formula as previously described (28):…”

Section: One-step Growth Curvementioning

confidence: 99%

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Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Montso

Mlambo

Ateba

2019

Front. Public Health

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The increasing incidence of antibiotic resistance and emergence of virulent bacterial pathogens, coupled with a lack of new effective antibiotics, has reignited interest in the use of lytic bacteriophage therapy. The aim of this study was to characterize lytic Escherichia coli O177-specific bacteriophages isolated from cattle feces to determine their potential application as biocontrol agents. A total of 31 lytic E. coli O177-specific bacteriophages were isolated. A large proportion (71%) of these phage isolates produced large plaques while 29% produced small plaques on 0.3% soft agar. Based on different plaque morphologies and clarity and size of plaques, eight phages were selected for further analyses. Spot test and efficiency of plating (EOP) analyses were performed to determine the host range for selected phages. Phage morphotype and growth were analyzed using transmission electron microscopy and the one-step growth curve method. Phages were also assessed for thermal and pH stability. The spot test revealed that all selected phages were capable of infecting different environmental E. coli strains. However, none of the phages infected American Type Culture Collection (ATCC) and environmental Salmonella strains. Furthermore, EOP analysis (range: 0.1-1.0) showed that phages were capable of infecting a wide range of E. coli isolates. Selected phage isolates had a similar morphotype (an icosahedral head and a contractile tail) and were classified under the order Caudovirales, Myoviridae family. The icosahedral heads ranged from 81.2 to 110.77 nm, while the contractile tails ranged from 115.55 to 132.57 nm in size. The phages were found to be still active after 60 min of incubation at 37 and 40 • C. Incremental levels of pH induced a quadratic response on stability of all phages. The pH optima for all eight phages ranged between 7.6 and 8.0, while at pH 3.0 all phages were inactive. Phage latent period ranged between 15 and 25 min while burst size ranged from 91 to 522 virion particles [plaque-forming unit (PFU)] per infected cell. These results demonstrate that lytic E. coli O177-specific bacteriophages isolated from cattle feces are highly stable and have the capacity to infect different E. coli strains, traits that make them potential biocontrol agents.

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Section: Discussionsupporting

confidence: 93%

Section: One-step Growth Curvementioning

confidence: 99%

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Montso

Mlambo

Ateba

2019

Front. Public Health

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“…The eclipse time, latent period, and the burst size were about 10 min, approximately 50 min and 133 ± 23 PFU/cell, respectively ( Figure 2D). Compared to literature values, the latent period for LPSEYT is shorter than other myoviruses, such as Salmonella phage vB_SnwM_CGG4-2 (about 58 min), Vibrio harveyi phage VhKM4 (about 60 min), and Stenotrophomonas maltophilia phage ϕSMA5 (about 80 min) [39][40][41]. At the same time, its burst size was greater than VhKM4 (about 52 PFU/cell), phSE-1 (about 28 PFU/mL), and phSE-2 (about 53 PFU/mL) [28,40], which is likely to be a reflection of high lytic activity.…”

Section: Morphology and Biological Characteristics Of Lpseytcontrasting

confidence: 58%

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

et al. 2020

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Salmonella is a leading cause of foodborne diseases, and in recent years, many isolates have exhibited a high level of antibiotic resistance, which has led to huge pressures on public health. Phages are a promising strategy to control food-borne pathogens. In this study, one of our environmental phage isolates, LPSEYT, was to be able to restrict the growth of zoonotic Salmonella enterica in vitro over a range of multiplicity of infections. Phage LPSEYT exhibited wide-ranging pH and thermal stability and rapid reproductive activity with a short latent period and a large burst size. Phage LPSEYT demonstrated potential efficiency as a biological control agent against Salmonella in a variety of food matrices, including milk and lettuce. Morphological observation, comparative genomic, and phylogenetic analysis revealed that LPSEYT does not belong to any of the currently identified genera within the Myoviridae family, and we suggest that LPSEYT represents a new genus, the LPSEYTvirus. This study contributes a phage database, develops beneficial phage resources, and sheds light on the potential application value of phages LPSEYT on food safety.

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“…It involves microbial cleanup of environmental pollutants without disruption to the local environment (Vezzulli et al, 2004). Bacteriophages with lytic activity are a novel tool for controlling bacterial pathogens (El-Dougdoug et al, 2019). In addition to that, using phage cocktail has a great effect in bio-desorption, bioremediation of pollutants and recycling of wastewater (Gaikwad et al, 2014;Zhao et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Improving Wastewater Treatment Using Dried Banana Leaves and Bacteriophage Cocktail

et al. 2019

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Control of Salmonella Newport on cherry tomato using a cocktail of lytic bacteriophages

Cited by 61 publications

References 76 publications

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

Improving Wastewater Treatment Using Dried Banana Leaves and Bacteriophage Cocktail

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