Improving Wastewater Treatment Using Dried Banana Leaves and Bacteriophage Cocktail

El-Dougdoug, Noha K.; Nasr-Eldin, Mohamed A.; Azzam, Mohamed I.; Mohamed, Aya; Hazaa, Mahmoud

doi:10.21608/ejbo.2019.7597.1295

Cited by 4 publications

(13 citation statements)

References 23 publications

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“…All isolated phages speci c for E.coli and P.aeruginosa form a circular, clear plaque with a diameter of 1mm to 5mm, which is consistent with the ndings of El-Dougdoug et al [39], who found that all isolated phages from wastewater samples collected from New Cairo, Gabal El-Asfar, and Helwan formed circular plaques with diameters ranging from 1mm to 5mm. C1, C2, and C3 are the names of the E. coli-speci c phages while P. aeruginosa-speci c phages were known as Ps1, Ps2, and Ps3.…”

Section: Discussionsupporting

confidence: 89%

“…After enrichment, phages speci c for E. coli strains and P. aeruginosa strains were collected from all studied sites with concentrations ranging from 10 7 to 10 9 pfu ml − 1 for E. coli and 10 6 to 10 8 pfu ml − 1 for P. aeruginosa phages, respectively, and designed as C1 to C3 phages speci c for E. coli and Ps1 to Ps3 phages speci c for P. aeruginosa. Due to the variance in density of bacteria speci c to phages, the titre of phages for E. coli and P. aeruginosa (10 2 pfu ml − 1 ) detected by El-Dougdoug et al [39] was so far from these results. On the other hand, these ndings were similar to those reported by Mahgoub et al [38], who collected sewage water from wastewater treatment plants (WTs) in Egypt's El-Sharkia Governorate.…”

Section: Discussionmentioning

confidence: 86%

“…The removal e ciency (percent) for E. coli and P. strains increased throughout time and peaked at hours after incubation with the phages cocktail. After 24 hours of treatment with a mixture of phages, E. coli and P. aeruginosa microorganisms in wastewater samples showed a 99 percent reduction [38][39]33].…”

Section: Discussionmentioning

confidence: 99%

“…DNA was isolated and ampli ed using the traditional PCR method with particular primer sequences for E. coli and P. aeruginosa 16s rDNA genes. Several studies have used the 16s rDNA gene sequence to investigate the genetic diversity of E. coli and P. aeruginosa isolates [38][39]33]. Using the DNAMAN tool, the sequences were matched to bacterial species in the GenBank database and identi ed as P. aeruginosa strain R2 (Accession No.…”

Section: Discussionmentioning

confidence: 99%

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Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

Soliman¹,

Othman²,

Shoman³

et al. 2022

Preprint

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In areas with limited water resources, the reuse of treated drainage water for non-potable purposes is increasingly recognised as a valuable and sustainable water resource. Numerous pathogenic bacteria found in drainage water have a detrimental impact on public health. The emergence of antibiotic-resistant bacteria and the current worldwide delay in the production of new antibiotics may make the issue of this microbial water pollution even more challenging. This challenge aided the resumption of phage treatment to address this alarming issue. In this study, strains of Escherichia coli and Pseudomonas aeruginosa and their phages were isolated from drainage and surface water from Bahr El-Baqar and El-Manzala Lake in Damietta governorate, Egypt. Bacterial strains were identified by microscopical and biochemical examinations which were confirmed by 16S rDNA gene sequencing. The susceptibility of these bacteria to several antibiotics revealed that most of the isolates had multiple antibiotic resistances (MAR). The calculated MAR index values (> 0.25) categorized study sites as potentially hazardous to health. Lytic bacteriophages against these multidrug resistant strains of E. coli and P. aeruginosa were isolated and characterized. The isolated phages were found to be pH and heat stable and were all members of the Caudovirales order as recognized by electron microscope. They infect 88.9% of E. coli strains and 100% of P. aeruginosa strains examined. Under laboratory conditions, the use of a phage cocktail resulted in a considerable reduction in bacterial growth. The removal efficiency (%) for E. coli and P. aeruginosa colonies increased with time and maximized at 24 hr revealing nearly 100% reduction after incubation with the phage mixture. The study candidates new phages for detecting and control other bacterial pathogens of public health concern to limit water pollution and maintain adequate hygiene.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

Soliman¹,

Othman²,

Shoman³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The suspension was used when heavily turbid, with optical density equivalent to a bacterial count of 10 9 CFU/ml ( Gayar et al., 2014 ). For phage isolation, TSB was prepared as double strength broth, especially supplemented with traces of calcium carbonate and magnesium sulfate heptahydrate ( Serwer et al., 2004 ; El-Dougdoug et al., 2019 ; Ács et al., 2020 ). A mixture of the bacterial host inoculum, the environmental sample, and the isolation medium at a ratio of 1:1:10, respectively, was incubated overnight at 28°;C, 180 rpm ( Hussein et al., 2019 ).…”

Section: Methodsmentioning

confidence: 99%

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Abd-Allah

El-Housseiny

Alshahrani

et al. 2022

Front. Cell. Infect. Microbiol.

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Accumulating evidence has denoted the danger of resistance in tenacious organisms like methicillin-resistant Staphylococcus aureus (MRSA). MRSA, a supple bacterium that adopts a variety of antibiotic resistance mechanisms, is the cause of multiple life-threatening conditions. Approaching a post-antibiotic era, bacteria-specific natural predators, bacteriophages, are now given the chance to prove eligible for joining the antibacterial weaponry. Considering the foregoing, this study aimed at isolating bacteriophages with promising anti-MRSA lytic activity, followed by characterization and optimization of the production of the bacteriophage with the broadest host range. Five phages were isolated from different environmental sources including the rinse of raw chicken egg, raw milk, and, remarkably, the raw meat rinses of chicken and fish. Examined for lytic activity against a set of 23 MRSA isolates collected from various clinical specimens, all five phages showed relatively broad host ranges with the bacteriophage originally isolated from raw fish rinse showing lytic activity against all the isolates tested. This phage is suggested to be a member of Siphoviridae family, order Caudovirales, as revealed by electron microscopy. It also exhibited good thermal stability and viability at different pH grades. Moreover, it showed reasonable stability against UV light and all viricidal organic solvents tested. Optimization using D-optimal design by response surface methodology was carried out to enhance the phage yield. The optimum conditions suggested by the generated model were a pH value of 7, a carbon source of 0.5% w/v sucrose, and a nitrogen source of 0.1% w/v peptone, at a temperature of 28°C and a bacterial inoculum size of 107 CFU/ml, resulting in a 2 log-fold increase in the produced bacteriophage titer. Overall, the above findings indicate the lytic ability inflicted by this virus on MRSA. Apparently, its stability under some of the extreme conditions tested implies its potential to be a candidate for pharmaceutical formulation as an anti-MRSA therapeutic tool. We hope that bacteriophages could tip the balance in favor of the human front in their battle against multidrug-resistant pathogens.

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Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

et al. 2023

View full text Add to dashboard Cite

In areas with limited water resources, the reuse of treated drainage water for non-potable purposes is increasingly recognised as a valuable and sustainable water resource. Numerous pathogenic bacteria found in drainage water have a detrimental impact on public health. The emergence of antibiotic-resistant bacteria and the current worldwide delay in the production of new antibiotics may make the issue of this microbial water pollution even more challenging. This challenge aided the resumption of phage treatment to address this alarming issue. In this study, strains of Escherichia coli and Pseudomonas aeruginosa and their phages were isolated from drainage and surface water from Bahr El-Baqar and El-Manzala Lake in Damietta governorate, Egypt. Bacterial strains were identified by microscopical and biochemical examinations which were confirmed by 16 S rDNA sequencing. The susceptibility of these bacteria to several antibiotics revealed that most of the isolates had multiple antibiotic resistances (MAR). The calculated MAR index values (> 0.25) categorized study sites as potentially hazardous to health. Lytic bacteriophages against these multidrug-resistant strains of E. coli and P. aeruginosa were isolated and characterized. The isolated phages were found to be pH and heat stable and were all members of the Caudovirales order as recognized by the electron microscope. They infect 88.9% of E. coli strains and 100% of P. aeruginosa strains examined. Under laboratory conditions, the use of a phage cocktail resulted in a considerable reduction in bacterial growth. The removal efficiency (%) for E. coli and P. aeruginosa colonies increased with time and maximized at 24 h revealing a nearly 100% reduction after incubation with the phage mixture. The study candidates new phages for detecting and controlling other bacterial pathogens of public health concern to limit water pollution and maintain adequate hygiene.

show abstract

Improving Wastewater Treatment Using Dried Banana Leaves and Bacteriophage Cocktail

Cited by 4 publications

References 23 publications

Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Biocontrol of multi-drug resistant pathogenic bacteria in drainage water by locally isolated bacteriophage

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