Control ofCryptococcus GattiiBiofilms by an Ethanolic Extract ofCochlospermum Regium(Schrank) Pilger Leaves

Abstract

Cryptococcus gattii is an etiologic agent of cryptococcosis and a serious disease that affects immunocompromised and immunocompetent patients worldwide. The therapeutic arsenal used to treat cryptococcosis is limited to a few antifungal agents, and the ability of C. gattii to form biofilms may hinder treatment and decrease its susceptibility to antifungal agents. The objective of this study was to evaluate the antifungal and antibiofilm activities of an ethanolic extract of Cochlospermum regium (Schrank) Pilger leaves against C. gattii. The antifungal activity was assessed by measuring the minimum inhibitory concentration (MIC) using the broth microdilution technique and interaction of the extract with fluconazole was performed of checkerboard assay. The antibiofilm activity of the extract was evaluated in 96-well polystyrene microplates, and the biofilms were quantified by counting colony forming units. The extract showed antifungal activity at concentrations of 62.5 to 250 μg/mL and when the extract was evaluated in combination with fluconazole, C. gattii was inhibited at sub-MIC levels. The antibiofilm activity of the extract against C. gattii was observed both during biofilm formation and on an already established biofilm. The results showed that the ethanolic extract of the leaves of C. regium shows promise for the development of antifungal drugs to treat cryptococcosis and to combat C. gattii biofilms.

Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

“…In traditional medicine C regium xylopodium, is used to cure gastric problems, ulcers, rheumatism, vaginal discharge, prostatic infections, abscesses, in analgesia, anti-hypertensive, antiinflammatory, in the treatment of arthritis and acne (Carvalho et al, 2018) . Studies evaluating the phytomedicinal properties for xylopodium and leaves showed important biological activities explored mainly from hydroethanolic, hydromethanolic, ethanolic, methanolic extracts and their fractions, as an antimicrobial agent on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida tropicalis and Cryptococcus gattii (Leme et al, 2017;Carvalho et al, 2018;Almeida-Apolonio et al, 2018).…”