Control of Cyclic AMP Accumulation in Dog Acini: Its Relation to Amylase Release

Abstract

In dispersed acini from dog pancreas, exogenous derivatives of cAMP stimulated the amylase release; 8-Br-cAMP was a more potent stimulant than Bt₂-cAMP, and induced a potentiation of the amylase release stimulated by cerulein. Both secretin and vasoactive intestinal peptide (VIP), increased cellular cAMP, with a greater potency for secretin, and supra-maximal concentration of secretin plus VIP increased cellular cAMP to the same degree as that obtained with secretin alone. ¹²⁵I-VIP-binding studies showed that in dog pancreatic acini there is a 95 % decrease in the number of VIP-preferring receptors with minor changes in receptor affinity as compared to guinea-pig pancreas. The absence of effect between secretin or VIP and cerulein in stimulating amylase release could likely be explained by the low number in VIP-preferring receptors on dog pancreatic acini.