Relationship of CCK/gastrin receptor binding to amylase release in dog pancreatic acini

Fourmy, Daniel; Zahidi, Ahmed; Pradayrol, Lucien; Vayssette, Jacqueline; Ribet, A

doi:10.1016/0167-0115(84)90053-3

Cited by 27 publications

(10 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 1984, CCK receptors having pharmacological features different from those of the rodent pancreatic receptor were found on pancreatic acini from the dog [216]. Indeed, gastrin, and the tetrapeptide common to gastrin and CCK, were only 25-fold and 100-fold less potent, respectively, than CCK-8 in inhibiting CCK radioligand binding to these receptors.…”

Section: The Cck-b Receptor In the Normal Pancreas And The Ar4-2j Celmentioning

confidence: 99%

The peripheral cholecystokinin receptors

Silvente-Poirot

Dufresne

Vaysse

et al. 1993

European Journal of Biochemistry

Self Cite

125

View full text Add to dashboard Cite

Section: The Cck-b Receptor In the Normal Pancreas And The Ar4-2j Celmentioning

confidence: 99%

The peripheral cholecystokinin receptors

Silvente-Poirot

Dufresne

Vaysse

et al. 1993

European Journal of Biochemistry

Self Cite

125

View full text Add to dashboard Cite

“…Binding Studies were radioiodinatcd by conjugation of the peptide to ,25I-BH reagent and purified by reverse-phase high performance liquid chromatography as previously de scribed [8], Binding assays were carried out on iso lated cells as described [6], ODC Assay ODC activity was measured as previously de scribed [6] with minor modifications. Cytosolic ex tracts were prepared by scraping off cells from dishes, and lysis was performed by freezing and thawing cells in buffer containing 100 mA/ Tris-HCl (pH 7.4), 1 mM EDTA and I mA/ dithiothreitol.…”

Section: Methodsmentioning

confidence: 99%

Gastrin Modulates Growth of a Rat Acinar Pancreatic Cell Line: Receptor Analysis and Signal Transduction

et al. 1990

View full text Add to dashboard Cite

Recent studies have demonstrated the presence of both CCK_A and CCK_B receptors on dog and guinea pig pancreas. Although CCK_A receptors are implicated in enzymatic secretion, biological effects of CCK_B receptors are still unknown. We have previously found that a rat acinar pancreatic cell line (AR4–2J) possesses both receptor subtypes. In this work we report the ability of various CCK/gastrin agonists and antagonists to bind with these receptors. We found that gastrin, pentagastrin and Gastrin/CCK4 induce ornithine decarboxylase activity, an early event involved in cell proliferation, as well as ³H-thymidine incorporation. Furthermore, these effects occur at doses at which these peptides interact only with the CCK_B receptor subtype. In view of these data we propose that modulation of AR4–2J cell growth by gastrin agonists specifically involve occupation of the CCK_B receptor.

show abstract

“…Additionally, no specific gastrin binding was demonstrated in pancreata of foetal rats (day 19 of gestation). In control adult rats, only by using very high amounts of membrane material and high ligand concentrations were specific '251-BH-de~-S03H-ga~trin (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) …”

Section: )mentioning

confidence: 99%

“…[Thr34,Ahx37]-CCK(31-39) (Ahx, aminohexanoic acid), human cles-S03H-[AhxlS]gastrin, des-S03H-gastrin(2 -17) and gastrin-(2-17) were synthesized as described in [13, 141. (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) and gastrin (2 -17) were radiodinated by conjugation with lz5I-BH and purified by reverse-phase HPLC, as described in [16]. The specific activity was 66.6-74.0 GBq/pmol.…”

Section: Chemicalsmentioning

confidence: 99%

Pharmacological and biochemical characterization of cholecystokinin/gastrin receptors in developing rat pancreas

Hadjiivanova

Dufresne

Silvente-Poirot

et al. 1992

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

Cholecystokinin/gastrin receptors in the pancreas of newborn (3-day-old) rats are of type A, as in control mature rats, revealed by pharmacological analysis of specific ' 251-Bolton-Hunter-reagent- day-old rats, 90 -125 kDa in 10-day-old rats and 85 -100 kDa in 14-day-old and 21 -day-old rats, as found in control adult rats. Endo-a-N-acetylglucosaminidase F treatment yielded a core protein of 42 kDa in all developmental stages. These findings are consistent with an age-related postnatal expression of distinct glycoforms of pancreatic cholecystokinin receptors. Furthermore, it was observed that the period 2-3 weeks after birth, characterized by stabilization of the mass of the cholecystokinin receptor, precedes the dramatic increase in the receptor number.Cholecystokinin (CCK) and gastrin are hormonal peptides that share the same C-terminal pentapeptide amino acid sequence [I]. Pancreatic acinar cells from dog and guinea pig have been shown to possess two types of CCK receptor [2 -61. The CCKA receptor type, has a high affinity for CCK, and the pancreatic CCKn or gastrin receptor type has a high affinity for gastrin and poorly discriminates between CCK and gastrin peptides.The existence of specific CCK receptors in term foetus and neonatal rat pancreas was previously documented, although acini or lobules seem to be unresponsive to CCK stimulation near birth [7-91. It was also shown that the capacity of the high-affinity sites on rat pancreatic acini, as well as amylase secretion due to CCK stimulation, gradually increases with

show abstract

Relationship of CCK/gastrin receptor binding to amylase release in dog pancreatic acini

Cited by 27 publications

References 18 publications

The peripheral cholecystokinin receptors

The peripheral cholecystokinin receptors

Gastrin Modulates Growth of a Rat Acinar Pancreatic Cell Line: Receptor Analysis and Signal Transduction

Pharmacological and biochemical characterization of cholecystokinin/gastrin receptors in developing rat pancreas

Contact Info

Product

Resources

About