Control and isotopic quantification of affinity of antithrombin III for heparin-like surfaces

Caix, J.; Migonney, Véronique; Baquey, A.; Fougnot, C.; Minnot, A. Perrot; Beziade, A.; Vuillemin, L; Baquey, C.; Ducassou, D.

doi:10.1016/0142-9612(88)90071-3

Cited by 13 publications

(3 citation statements)

References 10 publications

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“…These results are in agreement with the different morphologies of the surface observed in AFM and SEM (Figs. [2][3][4][5]. Indeed, better covering of the virgin polymer was observed when grafting and functionalization were performed with gamma radiation.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies [1][2][3][4][5] have reported that polystyrene (PS) derivatives with antithrombotic properties can be prepared. They are able to catalyze the inhibition of thrombin by antithrombin in the same way as heparin.…”

Section: Introductionmentioning

confidence: 99%

“…4 show the surface topography obtained by SEM of the two kinds of polymer [PVDF and P(VDF-HFP)] at each step of the synthesis: virgin, grafted, and functionalized. When the grafting time increased, the styrene grafting yield, Y, also increased rapidly during the first 2 h and finally reached a saturation level at about 16 h 18.…”

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confidence: 99%

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Development of ?heparin-like? polymers using swift heavy ion and gamma radiation. I. Preparation and characterization of the materials

Durrieu

Aymes-Chodur

Betz

et al. 2000

J. Biomed. Mater. Res.

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The development of ideal antithrombogenic polymers, a major problem in biomaterials sciences, is a primary objective in the fields of cardiovascular prostheses, artificial hearts, and other devices. To decrease their thrombogenicity, which remains the major obstacle, we have developed polymeric materials endowed with a specific affinity for antithrombin III (ATIII) and thus able, like heparin, to catalyze the inhibition of thrombin by ATIII. Sulfonate and sulfonamide groups are introduced onto phenyl rings belonging to styrene residues, which are radiation grafted (using swift heavy ion and gamma radiation) onto poly(vinylidene difluoride) (PVDF) and also onto poly(vinylidene fluoride/hexafluoropropylene) [P(VDF-HFP)]. In contrast to gamma radiation, which leads to a homogeneous modification, the advantage of swift heavy ion grafting is that only small regions are modified; thus, the surface may present hydrophilic (corresponding to the modified areas) and hydrophobic microdomains (corresponding to the unmodified areas) of different sizes, depending on the absorbed dose and grafting yield. Surface topography and composition are characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Sulfur, sodium, fluorine, and carbon are determined by scanning electron microscopy combined with energy-dispersive X-ray analysis (SEM-EDXA). The amount of fluorine decreases as polystyrene (PS) is grafted, whatever the kind of radiation and polymer. When the polymers are functionalized, the amount of fluorine also decreases while sodium and sulfur appear. Functionalization seems to increase the roughness of the surface, and its area.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of ?heparin-like? polymers using swift heavy ion and gamma radiation. I. Preparation and characterization of the materials

Durrieu

Aymes-Chodur

Betz

et al. 2000

J. Biomed. Mater. Res.

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Bioactive Polymers and Surfaces: A Solution for Implant Devices

Migonney¹

2014

Biomaterials

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Interaction of poly(sodium vinyl sulfonate) and its surface graft with antithrombin III

Ito

Liu

Imanishi

1991

J. Biomed. Mater. Res.

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Poly(sodium vinyl sulfonate) (PVS) was found to be 1/14 times as active as heparin in inducing the conformational change and activation of antithrombin III. The conformational change of antithrombin III was investigated in terms of the intrinsic fluorescence of tryptophan residue, the extrinsic fluorescence using 1,6-diphenyl-1,3,5-hexatriene as fluorescent probe, and Fourier-transform infrared spectroscopy. It was evident in the experiment using 2,4,6-trinitrobenzene sulfonate that PVS elicited the activity of antithrombin III by interacting with amino groups of the protein as does heparin. Sodium vinyl sulfonate was graft-polymerized onto polyetherurethane (PEU) film that was treated with glow discharge in advance. PVS-grafted PEU film adsorbed antithrombin III easily and, like ungrafted PVS, induced conformational change and activation of antithrombin III. However, the mechanism of interaction of the PVS graft with antithrombin III did not seem to be completely the same as that of ungrafted PVS in solution.

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Control and isotopic quantification of affinity of antithrombin III for heparin-like surfaces

Cited by 13 publications

References 10 publications

Development of ?heparin-like? polymers using swift heavy ion and gamma radiation. I. Preparation and characterization of the materials

Development of ?heparin-like? polymers using swift heavy ion and gamma radiation. I. Preparation and characterization of the materials

Bioactive Polymers and Surfaces: A Solution for Implant Devices

Interaction of poly(sodium vinyl sulfonate) and its surface graft with antithrombin III

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