Interaction of poly(sodium vinyl sulfonate) and its surface graft with antithrombin III

Ito, Yoshihiro; Liu, LinShu; Imanishi, Yukio

doi:10.1002/jbm.820250108

Cited by 12 publications

(4 citation statements)

References 10 publications

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“…pVS has been explored before as an antithrombogenic agent, , HIV-inhibitor, coatings for antibacterial surfaces, and as polyelectrolytes for antibody and protein purification processes, − but never before as a coactivator for FGFRs. From screening studies, we identified pVS from other polysulfonated polymers as a new heparin-mimicking polymer.…”

Section: Resultsmentioning

confidence: 99%

“…Various concentrations of pVS 80.3 kDa were incubated with HDF cells in the absence of bFGF for 24 h. Then a LIVE/DEAD viability/cytotoxicity assay was performed, which indicated that the polymer was not toxic to HDF cells up to at least 1 mg/mL. pVS has been explored before as an antithrombogenic agent, 37,38 HIV-inhibitor, 39 coatings for antibacterial surfaces, 40 and as polyelectrolytes for antibody and protein purification processes, 41−43 but never before as a coactivator for FGFRs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

et al. 2015

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Heparin is a highly sulfated polysaccharide and useful because of its diverse biological functions. However, because of batch-to-batch variability and other factors, there is significant interest in preparing biomimetics of heparin. To identify polymeric heparin mimetics, a cell-based screening assay was developed in cells that express fibroblast growth factor receptors (FGFRs) but not heparan sulfate proteoglycans. Various sulfated and sulfonated polymers were screened and poly(vinyl sulfonate) (pVS) was identified as the strongest heparin-mimicking polymer in its ability to enhance binding of basic fibroblast growth factor (bFGF) to FGFR. The results were confirmed by an ELISA-based receptor-binding assay. Different molecular weights of pVS polymer were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. The polymers were able to facilitate dimerization of FGFRs leading to cell proliferation in FGFR-expressing cells, and no size dependence was observed. The data showed that pVS is comparable to heparin in these assays. In addition, pVS was not cytotoxic to fibroblast cells up to at least 1 mg/mL. Together this data indicates that pVS should be explored further as a replacement for heparin.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

et al. 2015

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“…The amount of primary amine introduced into the HA chains was determined by spectrophotometric methods 20. Samples were reacted with 2,4,6,‐trinitrobenzenesulfonic acid (TNBS; Sigma) in 4% NaHCO 3 , pH 8.5, at 40°C for 4 h and neutralized to pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

Hyaluronate‐heparin conjugate gels for the delivery of basic fibroblast growth factor (FGF‐2)

Liu¹,

Ng²,

Thompson³

et al. 2002

J. Biomed. Mater. Res.

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The stability and activity of recombinant growth factors administered locally for the repair of damaged tissue can be directly influenced by the physical structure and chemical composition of the delivery matrix. This study describes a novel basic fibroblast growth factor-2 (FGF-2) delivery system synthesized by the conjugation of a structure-stabilizing polymer, hyaluronate (HA), with a sulfated glycosaminoglycan, heparin (HP), that has inherent specific binding sites for members of the FGF family. The biopolymers were formed via stable amine or labile imine bonds by coupling amine-modified HA with oxidized heparin. The addition of recombinant human FGF-2 resulted in the rapid binding of FGF-2 to the heparin segment of the hyaluronate-heparin (HAHP) conjugate. The FGF-2 was released in vitro from the imine-bonded (HAHPi) gels in the form of FGF-2-heparin complexes through the hydrolysis of the imine bonds. In contrast, the release of growth factor from the more stable amine-bonded (HAHPa) gels required treatment with free heparin or enzymatic digestion of the hyaluronate segment. Functional analysis of the released FGF-2 showed that the HAHP conjugate gels increased both the stability and activity of the growth factor.

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“…Heparin is a natural polyanionic polysaccharide carrying sulfonate groups ( , ). Therefore, several investigations using sulfonated polymers have been performed ( − ). Recently, it has been found that sulfated hyaluronic acid has a heparin-like property ( − ).…”

Section: Introductionmentioning

confidence: 99%

Photoimmobilization of Sulfated Hyaluronic Acid for Antithrombogenicity

et al. 1997

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Anticoagulant polymer sulfated hyaluronic acid was patterned immobilized on a poly(ethylene terephthalate) (PET) film in a specific pattern by photolithography. Hyaluronic acid was sulfated by a sulfur trioxide-pyridine complex. The polymer was coupled with azidoaniline. The derivatized polymer was cast on a PET film from aqueous solution. After drying, the film was photoirradiated in the presence or absence of a photomask. The micropatterning was confirmed by staining with a dye, brilliant green. Since the anticoagulant polymer has negative charges, the cationic dye was adsorbed on the regions where the anticoagulant polymer was immobilized. Platelet adhesion was reduced on the sulfated hyaluronic acid-immobilized areas. The immobilized sulfated hyaluronic acid significantly reduced thrombus formation.

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Interaction of poly(sodium vinyl sulfonate) and its surface graft with antithrombin III

Cited by 12 publications

References 10 publications

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

Poly(vinyl sulfonate) Facilitates bFGF-Induced Cell Proliferation

Hyaluronate‐heparin conjugate gels for the delivery of basic fibroblast growth factor (FGF‐2)

Photoimmobilization of Sulfated Hyaluronic Acid for Antithrombogenicity

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