Contributions of different intraarticular tissues to the acute phase elevation of synovial fluid MMP‐2 following rat ACL rupture

Tang, Zuoyin; Yang, Li; Wang, Yequan; Xue, Ruyue; Zhang, Jin; Huang, Wei; Chen, Peter C; Sung, ki-wol

doi:10.1002/jor.20763

Cited by 64 publications

(62 citation statements)

References 36 publications

(41 reference statements)

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“…30 Previously, another in vivo model study from our lab also revealed that intra-articular tissue have contributed to the accumulation of MMP-2 production and its elevated proteolytic activity in joint fluid after mechanical injury. 11 Since MMPs are largely involved in the breakdown of ECM in normal physiological processes as well as disease processes, this may suggest one mechanism by which the knee joint tissue are difficult to repair. The accumulation of MMP-2 in the joint fluid after injury might disrupt the delicate balance of removing damaged matrix components with the deposition of newly synthesized materials.…”

Section: Discussionmentioning

confidence: 99%

“…18 Earlier studies have shown that ACL fibroblast repair directly relates to MMP-2 production. 11 Previously, we have reported increased amount of TNF-a, IL-1b, and IL-6 in the joint fluid during the acute inflammatory phase after ACL injury. 11 These cytokines are also considered important chemical mediators in the acute inflammatory phase of wound healing.…”

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confidence: 90%

“…11 Previously, we have reported increased amount of TNF-a, IL-1b, and IL-6 in the joint fluid during the acute inflammatory phase after ACL injury. 11 These cytokines are also considered important chemical mediators in the acute inflammatory phase of wound healing. 19,20 Many studies have reported elevated expressions of cytokines and MMPs in tendon fibroblasts, synoviocytes with/without mechanical stretch/compression.…”

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confidence: 90%

“…11 Briefly, the protein concentration of each sample was measured using a BCA protein assay kit (Bioteke, Beijing, China). Same amount of total proteins from different samples were separated in 10% SDS-PAGE gel copolymerized with 0.05% gelatin.…”

Section: Signal Pathway Inhibitors Treatmentmentioning

confidence: 99%

“…10 Ruptured ACL is exposed to many inflammatory factors along with proteases particularly matrix-metalloproteinases (MMPs) that participate in extracellular matrix degradation. [11][12][13] Most of the MMP's are secreted as inactive pro-proteins. Their activation is tightly controlled by other membrane-type MMPs (MT-MMPs), tissue inhibitors of metalloproteinases (TIMPs), various cytokines, and growth factors in addition to various transcriptional and translational controls.…”

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confidence: 99%

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Combined effects of TNF‐α, IL‐1β, and HIF‐1α on MMP‐2 production in ACL fibroblasts under mechanical stretch: An in vitro study

Wang

Tang

Xue

et al. 2011

Journal Orthopaedic Research

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ABSTRACT:The dynamics between inflammatory factors, mechanical stress, and healing factors, in an intra-articular joint, are very complex after injury. Injury to intra-articular tissue [anterior cruciate ligament (ACL), synovium] results in hypoxia, accumulation of various pro-inflammatory factors, cytokines, and metalloproteases. Although the presence of increased amounts of matrix-metalloproteinases (MMP) in the joint fluid after knee injury is considered the key factor for ACL poor healing ability; however, the exact role of collective participants of the joint fluid on MMP-2 activity and production has not been fully studied yet. To investigate the combined effects of mechanical injury, inflammation and hypoxia induced factor-1a (HIF-1a) on induction of MMP-2; we mimicked the microenvironment of joint cavity after ACL injury. The results show that TNF-a and IL-1b elevate the activity of MMP-2 in a dose-and time-dependent manner. In addition, mechanical stretch further enhances the MMP-2 protein levels with TNF-a, IL-1b, and their mixture. CoCl 2 -induced HIF-1a (100 and 500 mM) also increases the levels and activity of MMP-2. Mechanical stretch has a strong additional effect on MMP-2 production with HIF-1a. Our results conclude that mechanical injury, HIF-1a and inflammatory factors collectively induce increased MMP-2 production in ACL fibroblasts, which was inhibited by NF-kB pathway inhibitor . ß

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Section: Discussionmentioning

confidence: 99%

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confidence: 90%

mentioning

confidence: 90%

Section: Signal Pathway Inhibitors Treatmentmentioning

confidence: 99%

mentioning

confidence: 99%

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Combined effects of TNF‐α, IL‐1β, and HIF‐1α on MMP‐2 production in ACL fibroblasts under mechanical stretch: An in vitro study

Wang

Tang

Xue

et al. 2011

Journal Orthopaedic Research

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Role of Nitric Oxide Production and Matrix Protease Activity in Cruciate Ligament Degeneration

Spreng¹

2010

Advances in the Canine Cranial Cruciate Ligament

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Groove model of tibia‐femoral osteoarthritis in the rat

Visser

Weinans

Coeleveld

et al. 2016

Journal Orthopaedic Research

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Several experimental models of osteoarthritis in rats are used to study the pathophysiology of osteoarthritis. Many mechanically induced models have the limitation that permanent joint instability is induced by, for example, ligament transection or meniscal damage. This permanent instability will counteract the potential beneficial effects of therapy. The groove model of osteoarthritis uses a one‐time trigger, surgically induced cartilage damage on the femoral condyles, and has been validated for the canine tibia‐femoral compartment. The present study evaluates this model for the rat knee joint. The articular cartilage of the weight bearing surface of both femoral condyles and trochlea were damaged (grooved) without damaging the underlying subchondral bone. Severity of joint degeneration was histologically assessed, in addition to patella cartilage damage, and subchondral bone characteristics by means of (contrast‐enhanced) micro‐CT. Mild histological degeneration of the surgically untouched tibial plateau cartilage was observed in addition to damage of the femoral condyles, without clear synovial tissue inflammation. Contrast enhanced micro‐CT demonstrated proteoglycan loss of the surgically untouched patella cartilage. Besides, a more sclerotic structure of the subchondral bone was observed. The tibia‐femoral groove model in a rat results in mild knee joint degeneration, without permanent joint instability and joint inflammation. This makes the rat groove model a useful model to study the onset and progression of post‐traumatic non‐inflammatory osteoarthritis, creating a relatively sensitive model to study disease modifying osteoarthritic drugs. © 2016 The Authors. Journal of Orthopaedic Research published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 35:496–505, 2017.

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Contributions of different intraarticular tissues to the acute phase elevation of synovial fluid MMP‐2 following rat ACL rupture

Cited by 64 publications

References 36 publications

Combined effects of TNF‐α, IL‐1β, and HIF‐1α on MMP‐2 production in ACL fibroblasts under mechanical stretch: An in vitro study

Combined effects of TNF‐α, IL‐1β, and HIF‐1α on MMP‐2 production in ACL fibroblasts under mechanical stretch: An in vitro study

Role of Nitric Oxide Production and Matrix Protease Activity in Cruciate Ligament Degeneration

Groove model of tibia‐femoral osteoarthritis in the rat

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