Contribution to the packing of liquid chromatographic microcolumns

1.1 μm Superficially porous particles for liquid chromatography

“…This variation in packing rate throughout the duration of bed formation typically produces a more loosely packed bed with a more rapid packing rate at the initiation of column packing [9]. The interparticle porosity of the packed bed has also been found to increase as the particle size monodispersity improves [10]. Further bed density variation can be found to depend on the kinetic energy of the particles while packing.…”

“…These difficulties with column packing have been found to be exacerbated in moving to smaller diameter particles which are expected to yield greater efficiency [1,2,10,11,13,17]. As the particle size is decreased, the surface chemistry of the particle becomes more important due to the increase of the surface area to volume ratio [10].…”

Mesoporous polybutadiene-modified zirconia for high-temperature packed capillary liquid chromatography: column preparation and temperature programming stability

“…Preparation of highly efficient packed capillary LC columns (h < 3) is generally more challenging than preparing conventional sized columns [39]. Mainly, it impose more stringent requirements to the homogeneity and pureness of the slurry, since the disparaging effect on column efficiency caused by irregularities in the packing structure, e.g.…”

Microcolumn liquid chromatography: instrumentation, detection and applications

“…Some research groups have tried to develop a better understanding of the packing processesincorporating colloid chemical studies, filtration theories and stress behavior during bed compression [69,[87][88][89][90]. Although certain trends could be assigned with these studies, a sound theoretical description and understanding of packing methods for microcolumns is not available yet.…”