1.1 μm Superficially porous particles for liquid chromatography

Blue, Laura E.; Jorgenson, James W.

doi:10.1016/j.chroma.2014.12.055

Cited by 47 publications

(21 citation statements)

References 36 publications

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“…Blue and Jorgenson [65] analyzed particle aggregation in different slurry solvents using in-solution microscopy and found that the most efficient column also showed the strongest particle aggregation in the slurry. Conclusions based on the free-slurry behavior need to be considered with care, as slurry flow and the actual pressure distribution can substantially modify conditions during the packing process.…”

Section: Resultsmentioning

confidence: 99%

Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns

Reising

Godinho

Jorgenson

et al. 2017

Journal of Chromatography A

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Column wall effects and the formation of larger voids in the bed during column packing are factors limiting the achievement of highly efficient columns. Systematic variation of packing conditions, combined with three-dimensional bed reconstruction and detailed morphological analysis of column beds, provide valuable insights into the packing process. Here, we study a set of sixteen 75 µm i.d. fused-silica capillary columns packed with 1.9 µm, C18-modified, bridged-ethyl hybrid silica particles slurried in acetone to concentrations ranging from 5 to 200 mg/mL. Bed reconstructions for three of these columns (representing low, optimal, and high slurry concentrations), based on confocal laser scanning microscopy, reveal morphological features associated with the implemented slurry concentration, that lead to differences in column efficiency. At a low slurry concentration, the bed microstructure includes systematic radial heterogeneities such as particle size-segregation and local deviations from bulk packing density near the wall. These effects are suppressed (or at least reduced) with higher slurry concentrations. Concomitantly, larger voids (relative to the mean particle diameter) begin to form in the packing and increase in size and number with the slurry concentration. The most efficient columns are packed at slurry concentrations that balance these counteracting effects. Videos are taken at low and high slurry concentration to elucidate the bed formation process. At low slurry concentrations, particles arrive and settle individually, allowing for rearrangements. At high slurry concentrations, they arrive and pack as large patches (reflecting particle aggregation in the slurry). These processes are discussed with respect to column packing, chromatographic performance, and bed microstructure to help reinforce general trends previously described. Conclusions based on this comprehensive analysis guide us towards further improvement of the packing process.

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Section: Resultsmentioning

confidence: 99%

Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns

Reising

Godinho

Jorgenson

et al. 2017

Journal of Chromatography A

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“…Preparation of the capillary UHPLC columns has been described previously in detail [8–14]. Modifications to the procedure will be highlighted here.…”

Section: Methodsmentioning

confidence: 99%

Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns

Godinho

Reising

Tallarek

et al. 2016

Journal of Chromatography A

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Slurry packing capillary columns for ultrahigh pressure liquid chromatography is complicated by many interdependent experimental variables. Previous results have suggested that combination of high slurry concentration and sonication during packing would create homogeneous bed microstructures and yield highly efficient capillary columns. Herein, the effect of sonication while packing very high slurry concentrations is presented. A series of six, 1 m × 75 μm internal diameter columns were packed with 200 mg/mL slurries of 2.02 μm bridged-ethyl hybrid silica particles. Three of the columns underwent sonication during packing and yielded highly efficient separations with reduced plate heights as low as 1.05.

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“…Jorgenson further evaluated the properties of 1.1 m shell particles having 187 Å pore size that were packed into 30 m ID capillaries, using electrochemical detection 70 . The particles were synthesised from colloidal silica and bonded with octadecyldimethylchlorosilane.…”

Section: Small Particles In Capillary Column Formatmentioning

confidence: 99%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

151

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1.1 μm Superficially porous particles for liquid chromatography

Cited by 47 publications

References 36 publications

Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns

Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns

Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

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