Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka, Nobuo; McCalley, David V.

doi:10.1021/acs.analchem.5b04093

Cited by 150 publications

(108 citation statements)

References 174 publications

(353 reference statements)

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“…could be more pronounced due to wall effects during the packing procedure. Commonly, a smaller psd is given as a reason why the packing of core–shell stationary phases is more uniform compared to fully porous particles leading to a reduced A‐term contribution . The efficiency of the monolithic column is comparable to 3.0 μm fully porous particles, which was also found by Olah et al.…”

Section: Resultssupporting

confidence: 63%

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Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

et al. 2016

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The efficiency of miniaturized liquid chromatography columns with inner diameters between 200 and 300 μm has been investigated using a dedicated micro-liquid chromatography system. Fully porous, core-shell and monolithic commercially available stationary phases were compared applying van Deemter and kinetic plot analysis. The sub-2 μm fully porous as well as the 2.7 μm core-shell particle packed columns showed superior efficiency and similar values for the minimum reduced plate heights (2.56-2.69) before correction for extra-column contribution compared to normal-bore columns. Moreover, the influence of extra-column contribution was investigated to demonstrate the difference between apparent and intrinsic efficiency by replacing the column by a zero dead volume union to determine the band spreading caused by the system. It was demonstrated that 72% of the intrinsic efficiency could be reached. The results of the kinetic plot analysis indicate the superior performance of the sub-2 μm fully porous particle packed column for ultra-fast liquid chromatography.

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Section: Resultssupporting

confidence: 63%

“…between 50 μm and 300 μm. Furthermore, the need for increased detection sensitivity in combination with MS favors miniaturized separation techniques because lower flow rates improve the ionization and ion desorption process .…”

Section: Introductionmentioning

confidence: 99%

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

et al. 2016

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“…[1][2][3][4][5][6][7][8][9][10][11] However, it is difficult to select the optimal packing support, and to find suitable separation conditions, given a specific pressure in an HPLC system; and peripheral studies on the theory have thus far been limited. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] In 2005, Desmet et al invented kinetic performance methods 28 by expanding both Bristow and Knox's, 29 and Poppe's 30 methods.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

2018

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There has been considerable discussion of the speed performance of HPLC separation, especially regarding the relationship between theoretical plates and hold-up time. The fundamental discussion focuses on the optimal velocity, u0,opt, which gives a minimal height equivalent to a theoretical plate of the van Deemter plot. On the other hand, Desmet's method, using the kinetic performance limit (KPL), calculates the highest performance with a constant pressure drop, without focusing solely on the optimal velocity. In this paper, a precise method based on the KPL is proposed, to understand how increasing pressure enhances both theoretical plates and hold-up time. A three-dimensional representation method that combines the pressure drop with two axes of time and theoretical plates will be useful for discussing the effect of pressure in pressure-driven chromatography. Using three dimensions, the methods based on u0,opt and the KPL can be combined, because u0,opt can be visualized three-dimensionally, including the neighbor of u0,opt; and the question of whether the KPL is an asymptotic or effective limit can be investigated. Three performances of high resolution, high speed, and low pressure can be understood on different packing supports at a glance.

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“…The development of modern LC has targeted fast and efficient separations, with improvements being made in innovative stationary phases and instrumentation . The stationary phase materials in LC can be generally classified into two categories: microspheres and monoliths . Monolith columns can offer fast separation and may be particularly useful for separation of large macromolecules.…”

Section: Introductionmentioning

confidence: 99%

Core–shell microspheres with porous nanostructured shells for liquid chromatography

Ahmed

Skinley

Herodotou

et al. 2017

J of Separation Science

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The development of new stationary phases has been the key aspect for fast and efficient high-performance liquid chromatography separation with relatively low backpressure.Core-shell particles, with a solid core and porous shell, have been extensively investigated and commercially manufactured in the last decade. The excellent performance of core-shell particles columns has been recorded for a wide range of analytes, covering small and large molecules, neutral and ionic (acidic and basic), biomolecules and metabolites. In this review, we first introduce the advance and advantages of coreshell particles (or more widely known as superficially porous particles) against nonporous particles and fully porous particles. This is followed by the detailed description of various methods used to fabricate core-shell particles. We then discuss the applications of common silica core-shell particles (mostly commercially manufactured), spheres-on-sphere particles and core-shell particles with a non-silica shell. This review concludes with a summary and perspective on the development of stationary phase materials for high-performance liquid chromatography applications.

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Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Cited by 150 publications

References 174 publications

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

Core–shell microspheres with porous nanostructured shells for liquid chromatography

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