Contribution of the phosphoenolpyruvate:mannose phosphotransferase system to carbon catabolite repression in Lactobacillus pentosus

Chaillou, Stéphane; Postma, Pieter W.; Pouwels, Peter H.

doi:10.1099/00221287-147-3-671

Cited by 37 publications

(38 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In CD630, gntR was upstream of genes encoding the mannose-specific phosphotransferase system (PTS) (Sebaihia et al, 2006). The mannose PTS is involved in sugar transport and global regulation of gene expression, in a number of Gram-positive genera (Abranches et al, 2003;Arous et al, 2004;Chaillou et al, 2001;Reizer et al, 1999), including the regulation of energy metabolism and virulence genes in Streptococcus mutans (Abranches et al, 2006). Hypothetically, integration of wC2 into CD630 could lead to significant changes in the C. difficile phenotype through mannose PTS deregulation.…”

Section: Discussionmentioning

confidence: 99%

The complete genome sequence of Clostridium difficile phage ϕC2 and comparisons to ϕCD119 and inducible prophages of CD630

et al. 2007

View full text Add to dashboard Cite

The complete genome sequence of Clostridium difficile phage wC2 and comparisons to wCD119 and inducible prophages of CD630 The complete genomic sequence of a previously characterized temperate phage of Clostridium difficile, wC2, is reported. The genome is 56 538 bp and organized into 84 putative ORFs in six functional modules. The head and tail structural proteins showed similarities to that of C. difficile phage wCD119 and Streptococcus pneumoniae phage EJ-1, respectively. Homologues of structural and replication proteins were found in prophages 1 and 2 of the sequenced C. difficile CD630 genome. A putative holin appears unique to the C. difficile phages and was functional when expressed in Escherichia coli. Nucleotide sequence comparisons of wC2 to wCD119 and the CD630 prophage sequences showed relatedness between wC2 and the prophages, but less so to wCD119. wC2 integrated into a gene encoding a putative transcriptional regulator of the gntR family. wC2, wCD119 and CD630 prophage 1 genomes had a Cdu1-attP-integrase arrangement, suggesting that the pathogenicity locus (PaLoc) of C. difficile, flanked by cdu1, has phage origins. The attP sequences of wC2, wCD119 and CD630 prophages were dissimilar. wC2-related sequences were found in 84 % of 37 clinical C. difficile isolates and typed reference strains. INTRODUCTIONClostridium difficile has emerged as an important intestinal pathogen since the 1970s, continuing to plague hospital settings worldwide and causing recent epidemics in the USA and Canada (Loo et al., 2005;Warny et al., 2005). The major virulence factors of C. difficile are toxins A and B encoded by tcdA and tcdB respectively, which are located on a 19 kb genomic region termed the pathogenicity locus (PaLoc) (Braun et al., 1996;Hammond & Johnson, 1995). The toxins are positively regulated by TcdR (Mani & Dupuy, 2001;Rupnik et al., 2005) and are negatively regulated by TcdC (Hundsberger et al., 1997;Matamouros et al., 2006); they are encoded by tcdR and tcdC respectively, also on the PaLoc. Another toxin-associated gene, tcdE, appears phage related but its function is unknown (Tan et al., 2001). C. difficile acquires antibiotic resistance and virulence genes through plasmids and transposons (Bruggemann, 2005) shown to significantly contribute to genome plasticity (Sebaihia et al., 2006). It is possible that phages also contribute to variance in virulence-associated genes (Lemee et al., 2005) and to the emergence of outbreak strains . However, the prevalence of phage genes within C. difficile genomes is not known. In comparison to phages of Escherichia coli, Staphylococcus aureus and Lactobacillus species, the study of clostridial phages is in its infancy. Only one phage specific for C. difficile, temperate phage wCD119, has been sequenced , while two putative prophage sequences were detected in the recently sequenced genome of C. difficile CD630 (Sebaihia et al., 2006).

show abstract

Section: Discussionmentioning

confidence: 99%

The complete genome sequence of Clostridium difficile phage ϕC2 and comparisons to ϕCD119 and inducible prophages of CD630

et al. 2007

View full text Add to dashboard Cite

show abstract

“…These results suggest that regulation of xylose utilization in L. brevis is not as stringently controlled as in other lactic acid bacteria, in which CCR operates to prioritize carbohydrate utilization more rigorously. 59 In lactic acid bacteria, Chaillou et al 60 demonstrated that transport and phosphorylation of glucose is undertaken by the mannose PTS (HPr, EI and an EII Man complex). Mutations in the EII Man complex inactivate it and elicit loss of the preferential consumption of glucose over other carbon sources, such as lactose in Lactobacillus casei or xylose in Tetragenococcus halophila.…”

Section: Bacillalesmentioning

confidence: 99%

“…The use of mutants affecting mannose PTS expression in S. salivarius has a pleiotropic effect on various metabolic enzymes, as well as on urease activity and on an inducible fructose PTS activity. 60 Interestingly, deletion of EII Man or mpt (mannose-specific PTS) also produces resistance or immunity to different class II bacteriocins. 54,[61][62][63] In addition, in Streptococcus gordonii, CcpA is implicated in penicillin tolerance both in vitro and in vivo, linking antibiotic survival to bacterial sugar metabolism.…”

Section: Bacillalesmentioning

confidence: 99%

Carbon source regulation of antibiotic production

et al. 2010

View full text Add to dashboard Cite

“…There is evidence for the presence of two glucose transport systems in L. monocytogenes, a high-affinity PTS and a low-affinity proton-motive-force-driven system (Parker & Hutkins, 1997). There are indications that the mannose PTS transports glucose in L. monocytogenes (Dalet et al, 2001) and this PTS is also known to transport mannose and 2-deoxyglucose (Chaillou et al, 2001;Romick et al, 1996). In many lactic acid bacteria and streptococci, Abbreviation: PTS, phosphoenolpyruvate-dependent phosphotransferase system.…”

Section: Introductionmentioning

confidence: 99%

“…In many lactic acid bacteria and streptococci, Abbreviation: PTS, phosphoenolpyruvate-dependent phosphotransferase system. transport and phosphorylation of glucose occurs mainly via a mannose PTS (Chaillou et al, 2001;Vadeboncoeur & Pelletier, 1997), which may be similar for L. monocytogenes.…”

Section: Introductionmentioning

confidence: 99%

Physiological implications of class IIa bacteriocin resistance in Listeria monocytogenes strains

et al. 2004

View full text Add to dashboard Cite

High-level resistance to class IIa bacteriocins has been directly associated with the absent EIIAB Man (MptA) subunit of the mannose-specific phosphoenolpyruvate-dependent phosphotransferase system (PTS) (EII Man t ) in Listeria monocytogenes strains. Class IIa bacteriocin-resistant strains used in this study were a spontaneous resistant, L. monocytogenes B73-MR1, and a defined mutant, L. monocytogenes EGDe-mptA. Both strains were previously reported to have the EIIAB Man PTS component missing. This study shows that these class IIa bacteriocin-resistant strains have significantly decreased specific growth and glucose consumption rates, but they also have a significantly higher growth yield than their corresponding wild-type strains, L. monocytogenes B73 and L. monocytogenes EGDe, respectively. In the presence of glucose, the strains showed a shift from a predominantly lactic-acid to a mixed-acid fermentation. It is here proposed that elimination of the EIIAB Man in the resistant strains has caused a reduced glucose consumption rate and a reduced specific growth rate. The lower glucose consumption rate can be correlated to a shift in metabolism to a more efficient pathway with respect to ATP production per glucose, leading to a higher biomass yield. Thus, the cost involved in obtaining bacteriocin resistance, i.e. losing substrate transport capacity leading to a lower growth rate, is compensated for by a higher biomass yield.

show abstract

Contribution of the phosphoenolpyruvate:mannose phosphotransferase system to carbon catabolite repression in Lactobacillus pentosus

Cited by 37 publications

References 35 publications

The complete genome sequence of Clostridium difficile phage ϕC2 and comparisons to ϕCD119 and inducible prophages of CD630

The complete genome sequence of Clostridium difficile phage ϕC2 and comparisons to ϕCD119 and inducible prophages of CD630

Carbon source regulation of antibiotic production

Physiological implications of class IIa bacteriocin resistance in Listeria monocytogenes strains

Contact Info

Product

Resources

About