Physiological implications of class IIa bacteriocin resistance in Listeria monocytogenes strains

Vadyvaloo, Viveka; Snoep, Jacky L.; Hastings, John W.; Rautenbach, Marina

doi:10.1099/mic.0.26731-0

Cited by 36 publications

(24 citation statements)

References 27 publications

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“…In many references in the literature, altered carbon metabolism has been linked to class IIa bacteriocin resistance (7,28,48,49). However, some other practical consequences for the field of bacteriocin production stem from the results of this work.…”

Section: Discussionmentioning

confidence: 50%

The Lcn972 Bacteriocin-Encoding Plasmid pBL1 Impairs Cellobiose Metabolism in Lactococcus lactis

Campelo

Gaspar

Roces

et al. 2011

Appl Environ Microbiol

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pBL1 is a Lactococcus lactis theta-replicating 10.9-kbp plasmid that encodes the synthetic machinery of the bacteriocin Lcn972. In this work, the transcriptomes of exponentially growing L. lactis strains with and without pBL1 were compared. A discrete response was observed, with a total of 10 genes showing significantly changed expression. Upregulation of the lactococcal oligopeptide uptake (opp) system was observed, which was likely linked to a higher nitrogen demand required for Lcn972 biosynthesis. Strikingly, celB, coding for the membrane porter IIC of the cellobiose phosphoenolpyruvate-dependent phosphotransferase system (PTS), and the upstream gene llmg0186 were downregulated. Growth profiles for L. lactis strains MG1363, MG1363/pBL1, and MG1363 ⌬celB grown in chemically defined medium (CDM) containing cellobiose confirmed slower growth of MG1363/pBL1 and MG1363 ⌬celB, while no differences were observed with growth on glucose. The presence of pBL1 shifted the fermentation products toward a mixed acid profile and promoted substantial changes in intracellular pool sizes for glycolytic intermediates in cells growing on cellobiose as determined by highpressure liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). Overall, these data support the genetic evidence of a constriction in cellobiose uptake. Notably, several cell wall precursors accumulated, while other UDP-activated sugar pools were lower, which could reflect rerouting of precursors toward the production of structural or storage polysaccharides. Moreover, cells growing slowly on cellobiose and those lacking celB were more tolerant to Lcn972 than cellobiose-adapted cells. Thus, downregulation of celB could help to build up a response against the antimicrobial activity of Lcn972, enhancing self-immunity of the producer cells.

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Section: Discussionmentioning

confidence: 50%

The Lcn972 Bacteriocin-Encoding Plasmid pBL1 Impairs Cellobiose Metabolism in Lactococcus lactis

Campelo

Gaspar

Roces

et al. 2011

Appl Environ Microbiol

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“…reduced glucose consumption, reduced production of lactate and elevated production of formate, acetate and ethanol compared to wild-type cells, have also been observed in resistant mutants of Li. monocytogenes (Naghmouchi et al, 2007;Vadyvaloo et al, 2004a). In resistant cells of Lc.…”

Section: Transport Systems Confer Immunitymentioning

confidence: 99%

Target recognition, resistance, immunity and genome mining of class II bacteriocins from Gram-positive bacteria

et al. 2011

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Due to their very potent antimicrobial activity against diverse food-spoiling bacteria and pathogens and their favourable biochemical properties, peptide bacteriocins from Gram-positive bacteria have long been considered promising for applications in food preservation or medical treatment. To take advantage of bacteriocins in different applications, it is crucial to have detailed knowledge on the molecular mechanisms by which these peptides recognize and kill target cells, how producer cells protect themselves from their own bacteriocin (self-immunity) and how target cells may develop resistance. In this review we discuss some important recent progress in these areas for the non-lantibiotic (class II) bacteriocins. We also discuss some examples of how the current wealth of genome sequences provides an invaluable source in the search for novel class II bacteriocins.

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“…In Listeria species, low-level resistance (two-to fourfold) to class IIa bacteriocins is caused by alterations in membrane lipid composition (13,49,50). High-level resistance (1,000-fold) in Listeria monocytogenes and Enterococcus faecalis results primarily from the loss of EII t Man , which is encoded by the mpt (mannose permease two) operon (14,26,38).…”

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confidence: 99%

Novel Activator of Mannose-Specific Phosphotransferase System Permease Expression in Listeria innocua , Identified by Screening for Pediocin AcH Resistance

Xue¹,

Hunter²,

Steinmetz³

et al. 2005

Appl Environ Microbiol

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To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 g/ml; 1,000-fold less susceptible than the wild type), in which the transposon integrated into the putative promoter of the lin0142 gene, was isolated. lin0142 is located immediately upstream of the mpt operon (mptA/mptC/mptD) that encodes the mannose-specific phosphoenolpyruvate-dependent phosphotransferase system permease EII t Man , which serves as a docking protein for class IIa bacteriocins. The transcription of the mpt operon is known to be positively controlled by 54 factor and ManR (a 54 -associated activator). Transcripts for lin0142 and mpt were undetectable in the G7 mutant, based on quantitative real-time reverse transcriptase PCR analysis. When the wild-type lin0142 gene was expressed at a 7.9-fold-elevated level in the mutant via a multicopy-number plasmid, the level of mpt mRNA became 70% higher than that in the wild-type strain. In addition, the complementation strain reverted back to the pediocin AcH-susceptible phenotype. The levels of manR and rpoN ( 54 ) mRNAs were not directly influenced by the level of lin0142 transcription. lin0142 is the only one of the three mpt regulatory genes whose transcription is induced, albeit slightly (1.2-fold), by glucose. The combined results show that the lin0142 gene encodes a novel activator of the mpt operon. The Lin0142 protein contains a winged-helix DNA-binding motif and is distantly related to the Crp-Fnr family of transcription regulators.

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Physiological implications of class IIa bacteriocin resistance in Listeria monocytogenes strains

Cited by 36 publications

References 27 publications

The Lcn972 Bacteriocin-Encoding Plasmid pBL1 Impairs Cellobiose Metabolism in Lactococcus lactis

The Lcn972 Bacteriocin-Encoding Plasmid pBL1 Impairs Cellobiose Metabolism in Lactococcus lactis

Target recognition, resistance, immunity and genome mining of class II bacteriocins from Gram-positive bacteria

Novel Activator of Mannose-Specific Phosphotransferase System Permease Expression in Listeria innocua , Identified by Screening for Pediocin AcH Resistance

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