Contribution of nitrated polycyclic aromatic hydrocarbons to the mutagenicity of ultrafine particles in the roadside atmosphere

Kawanaka, Youhei; Matsumoto, Emiko; Wang, Ning; Yun, Sun-Ja; Sakamoto, Kazuhiko

doi:10.1016/j.atmosenv.2008.06.032

Cited by 47 publications

(30 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, such compounds are highly stable in a solid phase, and some of them show higher mutagenicity (2 ϫ 10 5 times) and carcinogenicity (10 times) than PAHs. 29,33 Mutagenicity in the Salmonella Assay DCM PM 2.5 extracts turned out to be mutagenic with a significant dose-response effect in S. typhimurium strains used in experiments (Tables 5 and 6). However, their mutagenic potency differed according to the tester strain used.…”

Section: Ambient Levels Of Pahs and Nitro-pahsmentioning

confidence: 99%

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Piekarska

2010

Journal of the Air & Waste Management Association

View full text Add to dashboard Cite

Ambient air particulate matter less than 2.5 m in aerodynamic diameter (PM 2.5 ) samples were collected during summer and autumn using a Staplex high-volume air sampler. They were later extracted with dichloromethane in a Soxhlet apparatus. Polyaromatic hydrocarbon (PAH) content in extracts was determined by the high-performance liquid chromatography technique using fluorescence detection, whereas the nitro-PAH content was determined by gas chromatography using mass detection. Four Salmonella typhimurium strains (TA98, TA100, YG1041, and YG1042) were used in assays conducted with and without metabolic activation. The extracts were also tested with the SOS chromotest supplied by Environmental Biodetection Products Incorporated. The obtained results confirmed the Salmonella assay and the SOS chromotest usability for the purpose of atmospheric pollution monitoring within an urban agglomeration. The atmospheric pollution extracts under examination differed among each other regarding total content and percentage of individual compounds, depending on the season of sampling. The highest total PAH content and the highest nitro-PAH content in the tested samples as well as the most extensive range of detected compounds were found in the autumn season (heating season). The highest mutagenicity was noted for PM 2.5 samples collected in autumn. The high values of mutagenicity ratios and induction factors were obtained from assays carried out with and without metabolic activation, which is an argument for the presence of promutagens and direct mutagens. The YG1041 strain proved to be the most effective in detection of mutagenicity of the suspended dust extracts because of its notably high sensitivity to nitro-aromatic compounds. The SOS chromotest was very sensitive to a large spectrum of genotoxic air pollutants and showed a high degree of similarity with the results of the Salmonella assay. In comparison with the frequently used Ames test, the SOS chromotest enables quick analysis of the genotoxic effects of samples using only one tester strain. In addition, its miniaturized design decreases the consumption of tested samples.

show abstract

Section: Ambient Levels Of Pahs and Nitro-pahsmentioning

confidence: 99%

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Piekarska

2010

Journal of the Air & Waste Management Association

View full text Add to dashboard Cite

show abstract

“…Allen et al, 1996;Miguel et al, 2004;Kawanaka et al, 2004Kawanaka et al, , 2008Albinet et al, 2008a;Saarnio et al, 2008;Schnelle-Kreis et al, 2001;Duan et al, 2005;Venkataraman and Friedlander, 1994;Venkataraman et al, 2002). These studies, conducted in various countries, showed that the PAH particle size distribution depended on several parameters, such as season or particle aging.…”

Section: Introductionmentioning

confidence: 99%

Particle size distribution of nitrated and oxygenated polycyclic aromatic hydrocarbons (NPAHs and OPAHs) on traffic and suburban sites of a European megacity: Paris (France)

et al. 2012

View full text Add to dashboard Cite

Abstract. The size distribution of particulate nitrated and oxygenated polycyclic aromatic hydrocarbons (NPAHs and OPAHs) was determined during two field campaigns at a traffic site in summer 2010 and at a suburban site during the MEGAPOLI (Megacities: Emissions, urban, regional and Global Atmospheric POLlution and climate effects, and Integrated tools for assessment and mitigation) experiment in summer 2009. Both, OPAHs and NPAHs were strongly associated (>85 %) to fine particles (D p < 2.5 µm) increasing the interest of their study on a sanitary point of view. Results showed really different NPAH and OPAH particle size distributions between both sites. At traffic site, clearly bimodal (notably for NPAHs) particle size distributions (D p = 0.14 and 1.4 µm) were observed, while the particle size distributions were more scattered at the suburban site, especially for OPAHs. Bimodal particle size distribution observed at traffic site for the NPAH could be assigned to the vehicle emissions and the particle resuspension. Broadest distribution observed at the suburban site could be attributed to the mass transfer of compounds by volatilization/sorption processes during the transport of particles in the atmosphere. Results also showed that the combination of the study of particle size distributions applied to marker compounds (primary: 1-nitropyrene; secondary: 2-nitrofluoranthene) and to NPAH or OPAH chemical profiles bring some indications on their primary and/or secondary origin. Indeed, 1,4-anthraquinone seemed only primary emitted by vehicles while 7-nitrobenz[a]anthracene, benz [a]antracen7,12-dione and benzo [b]fluorenone seemed secondarily formed in the atmosphere.

show abstract

“…Los resultados observados se pueden atribuir a agentes mutagénicos presentes en el material particulado que pueden alterar el marco de lectura u originar sustitución de bases. Dichos agentes pueden ser derivados nitroaromáticos, HPA, metales y agentes cancerígenos amino (Kawanaka et al 2008, Ianistcki et al 2009). Gran parte de la actividad mutagénica encontrada en este estudio podría ser debida a dinitropirenos, compuestos que no requieren activación metabólica y lesionan el ADN originando pérdida o ganancia de bases (Kawanaka et al 2008).…”

Section: Resultados Y Discusiónunclassified

“…Dichos agentes pueden ser derivados nitroaromáticos, HPA, metales y agentes cancerígenos amino (Kawanaka et al 2008, Ianistcki et al 2009). Gran parte de la actividad mutagénica encontrada en este estudio podría ser debida a dinitropirenos, compuestos que no requieren activación metabólica y lesionan el ADN originando pérdida o ganancia de bases (Kawanaka et al 2008). Estos resultados son similares a los encontrados en las muestras de PM 2.5 del aire de la ciudad de Pamplona Norte de Santander (Meléndez et al 2012).…”

Section: Resultados Y Discusiónunclassified

Actividad Mutagénica Inducida Por Hidrocarburos Aromáticos Policíclicos en Muestras De PM2.5 en Un Sector Residencial De Villa Del Rosario-Norte De Santander, Colombia

Gélvez

Vargas

Parra

2016

Rev. Int. Contam. Ambie.

View full text Add to dashboard Cite

Palabras clave: cromatografía de gases, material particulado, ensayo de Ames, Salmonella typhimurium TA98 y TA100 RESUMENLos contaminantes del aire, especialmente el material particulado (MP), han sido investigados como posibles agentes responsables del cáncer de pulmón. Varios componentes del MP como los hidrocarburos aromáticos policíclicos (HAP) pueden contribuir con efectos adversos sobre la salud. La contaminación atmosférica junto con la ingesta de alimentos contaminados, son la vía primaria de la exposición humana a los HAP. Los HAP en general, son sospechosos de ser carcinogénicos, aunque el nivel sea muy bajo. La principal característica sobre la salud, es su capacidad de inducir cáncer en los organismos expuestos. El transporte vehicular es la fuente más importante de emisiones contaminantes atmosféricas y contribuye con más del 60 % de las emisiones de HAP. El objetivo de este trabajo fue determinar la mutagenicidad de muestras de PM 2.5 , provenientes del aire de Villa del Rosario, norte de Santander, Colombia e identificar los HAP. La identificación se realizó por medio de cromatografía de gases con detector de ionización de llama. Los ensayos mutagénicos se realizaron por el prueba de Ames en cepas de Salmonella typhimurium TA98 y TA 100.Key words: gas chromatography, particulate matter, Ames test, Salmonella typhimuriium TA98 and TA100 ABSTRACTAir pollutants, especially particulate matter (PM) have been investigated as potential agents responsible for lung cancer. Several components of the PM such as polycyclic aromatic hydrocarbons (PAHs) can contribute to adverse health effects. Air pollution, along with the intake of contaminated foods, are the primary route of human exposure to PAHs. PAHs are generally suspected of being carcinogenic at some degree. Although the level is very low, the main health risk associated with PHAs, is its ability to induce cancer in exposed organisms. The vehicular transport is the largest and most important source of atmospheric emissions that contribute over 60 % of PAH emissions. The aim of this study was to determine the mutagenicity of PM2.5 samples, from the air in Villa Rev. Int. Contam.

show abstract

Contribution of nitrated polycyclic aromatic hydrocarbons to the mutagenicity of ultrafine particles in the roadside atmosphere

Cited by 47 publications

References 21 publications

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Particle size distribution of nitrated and oxygenated polycyclic aromatic hydrocarbons (NPAHs and OPAHs) on traffic and suburban sites of a European megacity: Paris (France)

Actividad Mutagénica Inducida Por Hidrocarburos Aromáticos Policíclicos en Muestras De PM2.5 en Un Sector Residencial De Villa Del Rosario-Norte De Santander, Colombia

Contact Info

Product

Resources

About