Contribution of Major Hydrophilic and Lipophilic Antioxidants from Papaya Fruit to Total Antioxidant Capacity

Abstract

Several studies have shown that phenolic and carotenoid compounds protect against oxidative stress, reducing the risk of cardiovascular diseases and some types of cancers. The objective of this research was to determine the individual contribution of the main phenolic compounds from the papaya fruit skin and the individual contribution of main carotenoids from the pulp for total antioxidant capacity at four ripening stages; and the individual and combined radical scavenging ability used the essays DPPH (radical 2, 2-diphenyl-1-picryhydrazyl) and ABTS (radical 2, 20-azino-bis (3-ethylbenzothiazoline)-6 sulphonic acid). Phenolic acids standards for this study were ferulic (FA), caffeic (CA) and p-coumaric (pCA) acids and carotenoids studied were Lycopene, β-carotene and β-cryptoxanthin. The phenol that showed the best DPPH• and ABTS•+ radical scavenging ability was CA, with 89.47% and 92.98%, respectively. Lycopene contributed the greatest to the TAC (11.9-43.2). Lycopene also showed the best DPPH• and ABTS•+ radical scavenging ability with 62.12% and 94.26%, respectively. Antiradical ability of phenolics and carotenoids depended on the structure of the compound and its concentration.

Phytochemical Properties of Processed Chickpea Varieties of Haryana (India)

“…According to Shahidi (2002) it was stated that positive and prominent correlation existed between catechin content and antioxidant activity in beach pea seed coats. As per finding of Sancho et al, 2013, some hydrophilic phenols and lipophilic phenols showed contribution towards total antioxidant activity in papaya fruit while others showed antagonistic effect. In conformity to the above results Yao et al, 2010 explained the existence of positive correlation in between total phenolics and antioxidant activity in seven different colored grains.…”

Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha

“…The scavenging of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or diammonium salt radical cation (ABTS+; Sigma-Aldrich) was determined based on literature procedure ( Sancho et al, 2013 ). The 2.4 mM Potassium persulfate (2.4 mM) 7 mM ABTS (7 mM) were mixed at a ratio of 1:1, protected from light with aluminum foil, and allowed to react for 14 h at ambient temperature (22 °C) and then labelled as.…”

Is it possible to use the DPPH and ABTS methods for reliable estimation of antioxidant power of colored compounds?

“…As results from the literature (Arnao 2000;Pérez-Jiménez et al 2008;Paśko et al 2009;Shalaby and Shanab 2013;Gayosso-García Sancho et al 2013), the mentioned methods should not be applied for the estimation of antioxidant properties of substances which themselves (or their oxidation products formed during neutralization of radicals) absorb in the range of the monitored wave length. The presence of such compounds in the measuring system falsifies the changes of absorbance resulting from the concentration decrease of the colored radical and makes the estimation of the real antioxidant activity of the examined compounds impossible.…”