Is it possible to use the DPPH and ABTS methods for reliable estimation of antioxidant power of colored compounds?

Olszowy, Małgorzata; Dawidowicz, Andrzej L.

doi:10.1007/s11696-017-0288-3

Cited by 77 publications

(56 citation statements)

References 22 publications

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“…Radical scavenging activities are essential because of the detrimental role of free radicals in foodstuffs and biological systems. ABTS and/or DPPH scavenging assays are common spectrophotometric methods for analyzing the radical scavenging activities (Gülçin et al, 2010;Olszowy & Dawidowicz, 2018). The DPPH assay offers an effective, quick and simple way to assess potential antioxidants.…”

Section: Antioxidant Capacitymentioning

confidence: 99%

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

et al. 2021

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The present study outlines the antioxidant and toxic activity, total phenol, flavonoid and tannin content of Digitalis ferruginea's extracts in addition to evaluating its proximate parameters and mineral elements. Successive extraction was made using different solvents (ethanol, acetone, water). Total phenol content was determined by the Folin-Ciocalteu's reagent method and flavonoid was determined by the aluminum chloride colorimetric method. The antioxidant activities were investigated using different assays. The phenolics were determined using HPLC. In addition an evaluation was made of the proximate parameters and mineral elements. The radical scavenging capacities were highest in the water extract. The same extract was effective in total antioxidant activities (β-carotene, 83.75%). The acetone extract demonstrated stronger reducing power and phosphomolybdenum antioxidant activity with 0.52 mg/mL and, 107.43 µg/mg, respectively. The HPLC results determined major phenolics: rutin and ferulic acid. This plant also have rich in polyphenolic content together with toxic activity and possesses nutrients and mineral elements. As well as demonstrating the good antioxidant activity of D. ferruginea this study suggest that the plant could be of particular interest from a practical perspective, as it is a significant source of potential natural antioxidants that can be used for the prevention of a range of diseases.

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Section: Antioxidant Capacitymentioning

confidence: 99%

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

et al. 2021

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“…Therefore, for determining the antioxidant activity of plant extracts different methods are applied in parallel. Among these, DPPH and ABTS radical scavenging methods are the most popular and most commonly used due to their sensitivity and ease [38]. These methods allow determining the activity of both hydrophobic and hydrophilic compounds [39,40].…”

Section: H 2 O 2 Content Antioxidant Enzymes Activity and Antioxidanmentioning

confidence: 99%

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

et al. 2020

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The aim of the study was to assess the effects of Melittis melissophyllum shading on its development and accumulation of phenolics. Their content (verbascoside, apiin, luteolin-7-O-glucoside, coumarin, 3,4-dihydroxycoumarin, o-coumaric acid 2-O-glucoside as well as o-coumaric, p-coumaric, chlorogenic, caffeic, ferulic and cichoric acid) was determined in the herb using HPLC-DAD. The results showed that the content of abovementioned flavonoids and phenolic acids was highest in plants grown under full sunlight. On the other hand, a higher content of coumarin was observed in shaded plants, especially after the seed-setting stage. A similar tendency was noted for the amount of chlorophyll a and b. The content of hydrogen peroxide and malondialdehyde, the activity of polyphenol oxidase and catalase and the antioxidant capacity of plant extracts (measured using DPPH, ABTS and FRAP assays) were found to be the highest in the plants grown in full sunlight. However, the plants grown in moderate (30%) shade were found to thrive best.

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“…The radical scavenging activity (RSA) of extruded products depends not only on the phenolic compounds but also on the interactions between these bioactive compounds and the food matrix [46]. The antioxidant capacity of extrudates and non-extruded control samples was determined using the DPPH assay, a sensitive electron-transfer reaction [47]. Figure 1c shows that the highest RSA (lowest IC 50 ) was obtained for samples processed at 70 • C and 150 rpm.…”

Section: Radical Scavenging Activitymentioning

confidence: 99%

In Vitro Antioxidant Activity Optimization of Nut Shell (Carya illinoinensis) by Extrusion Using Response Surface Methods

et al. 2019

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The pecan (Carya illinoinensis) nut shell is an important byproduct of the food processing industry that has not been previously explored as an antioxidant compound. This work aims to study the effect of the extrusion temperature and screw speed on the moisture content, water and oil absorption index, water solubility index, color, phenolic compounds, condensed tannin compounds, and antioxidant activity of pecan nut shell extrudates. Extrusion variables were adjusted using a response surface methodology. Extrusion, performed at 70 °C and 150 rpm, almost doubled the concentration of polyphenols in the non-extruded shell and significantly increased radical scavenging activity. Compounds in extrudates, performed at 70 °C and 150 rpm, were quantified by high-performance liquid chromatography (HPLC) with a diode-array detector (DAD) and identified by liquid chromatography coupled with time-of-flight mass spectrometry (LC-MSD-TOF). Extrusion significantly increased most phenolic acid compounds, including gallic acid, ellagic acid pentose, ellagic acid, dimethyl ellagic acid rhamnoside, and dimethyl ellagic acid. The soluble fiber in extrudates was more than three-fold higher than in the control. Therefore, extrusion at 70 °C and 150 rpm increased the concentration of phenolic compounds, antioxidant activity, and total dietary and soluble fiber. Our findings support the notion that extruded pecan nut shell can be used in clean-label products and improve their nutraceutical value.

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Is it possible to use the DPPH and ABTS methods for reliable estimation of antioxidant power of colored compounds?

Cited by 77 publications

References 22 publications

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

In Vitro Antioxidant Activity Optimization of Nut Shell (Carya illinoinensis) by Extrusion Using Response Surface Methods

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