Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring

Hassan, Sammer-ul; Nightingale, Adrian M.; Niu, Xize

doi:10.1039/c6an00620e

Cited by 42 publications

(60 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Absorption spectroscopyis one of the techniques used in microfluidics to quantify analytes of interest, which is described by Beer-Lambert law. Several LEDs could be used in combination with various filters to detect different analytes while the overall instrument cost can be still low [13,14]. Detection of several microchannels requires multiple LED/Photodiode arrays with multiple electrical connections in series.…”

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Hassan

Zhang

2020

Micromachines

Self Cite

View full text Add to dashboard Cite

Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.

show abstract

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Hassan

Zhang

2020

Micromachines

Self Cite

View full text Add to dashboard Cite

show abstract

“…The velocity can be also affected by an interplay of various physico‐chemical parameters like flow rate and interfacial tension . Hence, droplet characterization in real time is crucial, in particular for applications based on continuous droplet flow involving multipoint detection or multistep droplet systems .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Droplets in Microfluidic Devices: Simultaneous Determination of Velocity, Size and Content

et al. 2019

View full text Add to dashboard Cite

Microfluidic devices were designed to electrochemically detect in a two‐phase flow the velocity, size and content of aqueous droplets containing redox species. The principle of these determinations is based on the analysis of a unique chronoamperometric response recorded during the passage of a droplet over channel microelectrodes. Two configurations of electrochemical cell with different geometries were investigated both theoretically and experimentally. Velocity and size of droplets, as well as internal recirculating convection within droplets, were evaluated from chronoamperometric curves by specific transition times depending on the cell configuration. In addition, the droplet content was probed from the Faradaic current controlled by mass transport and by internal hydrodynamic regime. For droplet velocity and size, experimental data were systematically compared to optical measurements. All the results demonstrated the high performance of the electrochemical detection reached under these conditions. They successfully validate the concept of self‐consistent electrochemical detections of aqueous droplets within microchannels for the simultaneous determination of their velocity, size and content.

show abstract

“…Enzyme‐based biosensing is an area of great importance in areas as varied as point‐of‐care diagnostics, clinical monitoring, bioprocess technology, winemaking, or food processing . Multiscale porosity has become a fundamental feature for materials used as enzyme supports in biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Pérez-Anguiano

Pugin

Scolan

et al. 2017

Adv Funct Materials

View full text Add to dashboard Cite

Hierarchically porous transparent silica coatings combine large specific surface area with enhanced pore accessibility for optical biosensing. This paper describes a versatile approach to fabricate optically transparent silica coatings with multiscale porosity. Thin films (around 1 μm in thickness) of an aqueous suspension of primary silica aggregates form a mesoporous, interconnected matrix, and sacrificial polymer particles template well‐defined, discrete macropores with high structural integrity. The total surface area achieved is around 200 m2 g−1 with mesopore sizes of 20–40 nm and macropores of 250 nm, with a total porosity of 84%. The macro/meso dual range of porosity allows enhanced biocatalyst loadings of l‐lactate dehydrogenase for detection of lactate. The functionalized films showed a linear response within the range of interest of 1–20 × 10−3m of lactate. These biosensing coatings therefore strongly enhance sensitivity, speed and reliability of optically based lactate detection as compared to classical thin films with monomodal mesopore structure. Particle‐based simulations and experiments reveal that both the location and connectivity of the macropores control the biosensing performance. The coatings and procedure presented here are versatile, scalable, inexpensive, and are therefore compatible with a wide range of deposition techniques suitable for industrial and health care applications.

show abstract

Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring

Abstract: We present a miniaturised, field-deployable multi-detector flow cell to analyse enzyme kinetics in microdroplets.

Cited by 42 publications

References 35 publications

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Electrochemical Detection of Droplets in Microfluidic Devices: Simultaneous Determination of Velocity, Size and Content

Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Contact Info

Product

Resources

About