Continuous flow fast atom bombardment liquid chromatography/mass spectrometry: Studies involving conventional bore liquid chromatography with simultaneous ultraviolet detection

Abstract

A conventional bore liquid chromatograph has been interfaced to quadrupole and magnetic sector mass spectrometers configured for fast atom bombardment ionization via a continuous flow FAB probe. It is shown that post-column addition of FAB matrix and in-line ultraviolet detection facilities do not significantly compromise chromatographic integrity and that high quality mass spectra are obtainable from such FAB LC/MS studies.

Continuous-flow fast atom bombardment mass spectrometry of oligonucleotides

“…Because Grotjahn et al [15][16][17] used glycerol as the FAB matrix for the analysis of oligonucleotides and because glycerol solutions in aqueous methanol [12,24] or acetonitrile [10,11] have functioned satisfactorily as mobile phases for most other continuous-flow FAB applications, a mobile phase consisting of methanol, water, and glycerol was initially evaluated for the analysis of oligonucleotides by frlt-FAB mass spectrometry. The ratio of methanol, water, and glyc- Mass spectra illustrating the determination of the limits of detection for d(pC)s anddtp'T}, are shown in Figures 1 and 2, respectively.…”

“…In order to determine whether any ion suppression effects for oligonucleotide mixture~could be minimized by using frit-FAB mass spectrometry, exchange chromatography that requires high ionic strength buffers to elute oligonucleotides. Also overcome by this approach is the need for postcoiumn addition of matrix [27], which could circumvent the problem of high pH in the mobile phase during chromatography. Although only simple oligonucleotide mixtures have been investigated by LC/MS so far, the separation power of HPLC combined with the speed and convenience of on-line mass selective detection should make this LC/MS technique of great utility in the characterization of oligonucleotide mixtures.…”

“…Advantages of continuous-flow FAS and frit-FAB mass spectrometry over the standard FAB probe method of sample introduction include the ability to carry out on-line HPLC separations with detection by FAB mass spectrometry [5], reduced chemical noise from the FAS matrix [8], increased signal-to-noise ratio [8), and less suppression of sample ions when mixtures of samples are analyzed [9,10]. Examples of classes of compounds that have been analyzed by continuous-flow or frit-FAB LC/MS in clu de peptides [11][12][13], bile acids [6), and oligosaccharides [7). In addition, a series of mononucleotides and metallated mononucleotides has been analyzed by Bertrand et al [14] using continuous-flow FAS.…”

Nanoscale packed-capillary liquid chromatography coupled with mass spectrometry using a coaxial continuous-flow fast atom bombardment interface

“…The coupling of FAB with flowing liquid streams by Ito et al (Frit-FAB/MS) (24) and Caprioli et al (continuous-flow FAB/MS) (25) has provided a very useful interface between LC and FAB/MS (26)(27)(28)(29)(30)(31)(32). The success of these LC/continuous-flow FAB/MS interfaces led us to investigate the coupling of nanoscale LC columns with continuous-flow FAB.…”

Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules

“…The CF-FAB/MS interfaces developed thus far employ a single fused silica capillary column to transport the analytes to the FAB probe tip in the mass spectrometer. Addition of the matrix is accomplished either by adding the matrix to the analyte solutions or by postcolumn addition (3,4,8). However, precolumn addition of the matrix into the LC mobile phase can compromise the chromatography due to changes in the polarity and viscosity of the mobile phase.…”