Continuous-flow fast atom bombardment mass spectrometry of oligonucleotides

Breemen, Richard B. van; Martin, Linda D.; Le, John C.

doi:10.1016/1044-0305(91)80009-v

Cited by 12 publications

(2 citation statements)

References 27 publications

(40 reference statements)

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“…Van Breemen, Martin, & Le (1991) separated mixtures of oligodeoxynucleotides in a b-cyclodextrin-modified column with a mobile phase that contained methanol, water, and triethanolamine, and detected the analytes by continuous-flow frit-FAB-MS. Nevertheless, FAB-MS is generally restricted to small oligonucleotides, which excludes most of the nucleic acids of interest.…”

Section: A Ionization Techniques For Nucleic Acids Operable Under Comentioning

confidence: 99%

Analysis of nucleic acids by on‐line liquid chromatography–Mass spectrometry

Huber

Oberacher

2001

Mass Spectrometry Reviews

143

129

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The numerous problems posed by modern biochemistry, biology, and medicine, as well as the growing significance of genetic engineering require the application of fast and reliable methods of utmost sensitivity and selectivity for the analysis of nucleic acids. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) represent established analytical techniques for the characterization and structural elucidation of single- and double-stranded nucleic acids, ranging in size from a few nucleotides to several thousand base pairs. Although both techniques are independently applicable for nucleic acid analysis, the on-line hyphenation significantly enhances their potential for the robust and fully automable routine analysis of minute amounts of biological samples. Among the various chromatographic and mass spectrometric modes available in principle, ion-pair reversed-phase HPLC and electrospray ionization mass spectrometry (ESI-MS) have been shown to be the most suitable for the direct interfacing of liquid chromatography (LC) and MS. Instrumental setup, as well as chromatographic and mass spectrometric experimental conditions, need to be carefully selected in order to maximize the performance of the hyphenated analytical system. Applications of HPLC-ESI-MS include the characterization of oligodeoxynucleotides synthesized by solid-phase synthesis, the analysis of antisense oligodeoxynucleotides, oligonucleotide metabolites, and DNA adducts, the analysis of genomic segments specifically amplified by the polymerase chain reaction (PCR), the characterization of ribonucleic acids, the sizing of double-stranded DNA restriction fragments, the genotyping of short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), the detection of mutations in nucleic acid sequences, and the sequencing of nucleic acids.

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Section: A Ionization Techniques For Nucleic Acids Operable Under Comentioning

confidence: 99%

Analysis of nucleic acids by on‐line liquid chromatography–Mass spectrometry

Huber

Oberacher

2001

Mass Spectrometry Reviews

143

129

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“…Recently, FAB mass spectrometry has been combined online with reversed-phase HPLC in a system called continuous-flow FAB mass spectrometry. Continuous-flow FAB mass spectrometry has been applied to the analysis of a variety of compounds including peptides (8), oligonucleotides (9), and chlorophylls (10). However, no continuous-flow FAB mass spectrometric analyses of polyacetylenes or other conductive polymers have been reported.…”

Section: Introductionmentioning

confidence: 99%

Continuous-flow fast atom bombardment and field desorption mass spectrometry of oligomers of 3,3,3-trifluoro-1-phenylpropyne

Breemen¹,

Bumgardner²

1991

Anal. Chem.

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Field desorption mass spectrometry was used to determine the chain length, molecular weight range, and Identities of the end-capping groups of synthetic oligomers of 3,3,3-trlfluoro-1-phenylpropyne. In order to obtain structurally significant fragment Ions of selected molecular Ion precursors, the MS/MS technique of ß/ -llnked scanning with colllslonal activation (CAD) was used. Because of the short duration and low Intensity of the signal, no useful MS/MS data could be obtained using FD as the Ionization method. However, 5/E-Hnked scans following CAD could be obtained using fast atom bombardment (FAB) mass spectrometry. To overcome Ion "suppression" of components In the unpurified oligomeric mixture and reduce the chemical noise during FAB, continuous-flow FAB liquid chromatography/mass spectrometry (LC/MS) was used to obtain molecular weight Information comparable to field desorption mass spectrometry. Finally, B/E-linked scanning with CAD was carried out during LC/MS In order to obtain fragment ions confirming the presence of phenyl and trlfluoromethyl substituents on the polyacetylene chain.

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