LC‐MS Bioanalysis of Oligonucleotides

Bartlett, Mark; Chen, Buyun; McGinnis, Aidan

doi:10.1002/9781118671276.ch48

Cited by 4 publications

(2 citation statements)

References 111 publications

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“…It allows for seamless mass spectrometric (MS) study of large biomolecules, such as oligonucleotides, following their separation with liquid chromatography (LC) [1, 2]. However, the choice of optimal solvent systems has proven to be absolutely essential for the successful ionization of analytes by ESI.…”

Section: Introductionmentioning

confidence: 99%

The Role of Fluorinated Alcohols as Mobile Phase Modifiers for LC-MS Analysis of Oligonucleotides

Basiri

Hattum

Dongen

et al. 2016

J. Am. Soc. Mass Spectrom.

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Hexafluoroisopropanol (HFIP) has been widely used as an acidic modifier for mobile phases for liquid chromatography-mass spectrometry (LC-MS) analysis of oligonucleotides ever since the first report of its use for this purpose. This is not surprising considering the exceptional performance of HFIP compared to carboxylic acids which cause significant MS signal suppression in electrospray ionization. However, we have found that other fluorinated alcohols can also be utilized for mobile phase preparation and the choice of optimal fluorinated alcohol is determined by the ion-pairing (IP) agent. While HFIP is a very good choice to be used alongside less hydrophobic IP agents, other fluorinated alcohols such as 1,1,1,3,3,3-hexafluoro-2-methyl-2-propanol (HFMIP) can significantly outperform HFIP when used with more hydrophobic IP agents. We also found that more acidic fluorinated alcohols assist with the transfer of oligonucleotides with secondary structure (e.g. folded strands and hairpins) into the gas phase.

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Section: Introductionmentioning

confidence: 99%

The Role of Fluorinated Alcohols as Mobile Phase Modifiers for LC-MS Analysis of Oligonucleotides

Basiri

Hattum

Dongen

et al. 2016

J. Am. Soc. Mass Spectrom.

Self Cite

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show abstract

“…To find * * * ,..., , In research we will use the following well-known formulas the approximate formulas [7]: . At the end of the path there is a collision, in which a particle can be absorbed with a probability q  1 ; otherwise, she's moving on.…”

Section: Impact Factormentioning

confidence: 99%