Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes

Abstract

Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH• (1,1-diphenyl-2-picryl-hydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (–)- and (+)- catechin.

Influence of genotype and harvest year on polyphenol content and antioxidant activity in murtilla (Ugni molinae Turcz) fruit

“…Bolling et al (2010) found seasonal differences in total phenol and antioxidant constituents in almonds, reporting a 13% greater polyphenol content between different cultivars in 2005 compared to 2007. Because the qualitative and quantitative composition of phenolic compounds in fruits is unique to individual species and genotypes, it is possible to determine similarities and differences among standardized and confirmed genotypes and predict the response of new varieties to specific production and cultivation conditions (Sochor et al, 2010).…”

Automation of Methods for Determination of Lipid Peroxidation

“…Oxidative stress occurs in a situation, when the imbalance between the production of free radicals and effectiveness of antioxidant defence system occurs in a healthy organism. Determination of antioxidant activity or eventually markers directly connected with this variable is one way how to monitor the damage of organisms by these compounds [7][8][9][10][11][12][13][14]. The negative effect of free oxygen radicals consists in the lipid peroxidation.…”

Polyphenols and their Mechanism of Action in Allergic Immune ResponseImmune Response

“…Their antimutagenic, anticarcinogenic and anti-inflammatory effects have been confirmed as well [8][9][10][11].…”