Content of 8-Hydroxy-2-Deoxyguanosine in Steroid Receptor-Positive and Receptor-Negative Breast Cancer Cells

Abstract

The content of DNA damage marker 8-hydroxy-2-deoxyguanosine in 16 receptor-negative and 18 receptor-positive human breast neoplasms was measured by immunohistochemical methods. Positive staining was revealed in 81.3 and 50.0% samples of groups 1 and 2, respectively. The effect of arylhydrocarbon receptor agonist beta-naphthoflavone on the content of 8-hydroxy-2-deoxyguanosine and number of estrogen and progesterone receptors was evaluated in MCF-7 breast cancer cells. The degree of genotoxic damage significantly increased 1 h after combined treatment with estradiol and beta-naphthoflavone (in contrast to individual treatment) and remained practically unchanged in the follow-up period. According to the estrogen effect-switching phenomenon, genotoxic damage can contribute to the development of R(-)-breast cancer.

Increase in Cerebellar Neurotrophin-3 and Oxidative Stress Markers in Autism

“…1. While it is difficult to compare the 8-OH-dG between various published values due to different ways that the data are expressed, our 8-OH-dG values were several hundred-fold higher than those reported for the epithelial cells in culture [28]. The level of 8-OH-dG is increased from 0.47± 0.053 pmol/μg DNA in cerebellar tissue from control cases (n=6) to 0.768±0.276 pmol/μg in cerebellar tissue from autistic cases (n=5), which represents a 63.4% increase in 8-OH-dG levels in autism.…”

DNA Oxidation and Antioxidant Status in Breast Cancer

“…In an immunohistochemical study, positive staining for 8-OHdG was observed in 81.3% of receptor-positive (estrogen and progesterone) human breast neoplasms and 50.0% of receptor-negative human breast neoplasms. 20 …”

“…In an immunohistochemical study, positive staining for 8-OHdG was observed in 81.3% of receptor-positive (estrogen and progesterone) human breast neoplasms and 50.0% of receptor-negative human breast neoplasms. 20 Under physiological conditions, each tissue maintains a steady and consistent enzymatic pattern that is significantly altered in malignancy as a result of uncontrolled proliferation of cells. In a number of previous studies, G-Px and SOD activities were found to be increased in breast tumor tissues when compared with corresponding uninvolved adjacent tissues, 11,12,17 and activities of these enzymes were at a higher level in blood of breast cancer patients than healthy controls.…”

8‐Hydroxy‐2′‐deoxyguanosine (8‐OH‐dG) as a potential survival biomarker in patients with nonsmall‐cell lung cancer

“…Elevated levels of 8-OHdG have also been linked to increased risk of cancers in breast, bladder, and basal cell, etc. [26][27][28][29] However, it remains largely unknown whether the levels of 8-OH-dG have significant effects on survival or other prognostic variables in patients with cancer. In 72 patients with renal cell carcinoma, Miyake et al 30 found that cancer-specific survival in patients with an elevated 8-OH-dG was significantly lower than that in patients with a normal 8-OH-dG (P ¼ .032, log-rank test).…”