Contamination of the Shell and Internal Content of Table Eggs With Some Pathogens During Different Storage Periods

Mansour, Amal F.A.; Zayed, Amany F.; Basha, Ola

doi:10.21608/avmj.2015.169765

Cited by 13 publications

(8 citation statements)

References 26 publications

(11 reference statements)

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“…and none of the egg content samples were contaminated with this bacterium. The same results were reported by Mansour et al (2015) who recorded a total absence of Salmonella in the egg content samples. These observed variations can be explained by the different approaches used for the choice of samples (eggshell or egg content), and the methods used to isolate and identify the microorganism.…”

Section: Discussionsupporting

confidence: 87%

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Merati¹,

Boudra²

2023

WVJ

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Salmonellosis is a significant public health problem worldwide. The current study aimed to investigate the presence of Salmonella spp. in commercial eggs of Tiaret province, Algeria, and evaluate the susceptibility of isolated strains to different antimicrobial agents. A total of 180 commercial eggs collected from various retail outlets (groceries, butchers, wholesalers, street vendors) were analyzed by conventional methods, and 13 Salmonella spp. isolates were tested on a panel of 7 antimicrobial agents using the disc diffusion method. Of 180 chicken egg content samples examined, the findings indicated that 13 (7.22%) were positive for Salmonella spp. Regarding the collection site, 2 (1.11%), 4 (2.22%), and 7 (3.88%) of Salmonella spp. isolates were detected from butchers, wholesalers, and street vendors, respectively. Most antibiotic discs have demonstrated widespread resistance with an incidence rate of 100%, including amoxicillin + clavulanic acid, ampicillin, nalidixic acid, and erythromycin. However, colistin sulfate, gentamycin, and tetracycline were more effective against Salmonella isolates. It can be concluded that the highest detection rate of Salmonella spp. was observed for street vendors, and the highest resistance was recorded for commonly used antibiotics in poultry production.

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Section: Discussionsupporting

confidence: 87%

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Merati¹,

Boudra²

2023

WVJ

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“…However, in our study we used higher amounts of bacteria (inoculum: 10 6 CFU/ml), resulting in an L. monocytogenes load of 10 5 CFU/egg, and this load was depleted below our detection limits. Hannah et al () and Mansour et al () reported a prevalence of 68% for aerobic bacteria at loads of ~2.3 log 10 CFU/ml in eggshell rinsate. This model revealed that NEW could reduce bacterial load without affecting the cuticle or the mineral composition of the eggshell.…”

Section: Resultsmentioning

confidence: 99%

The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes

Rivera‐Garcia

Santos‐Ferro

Ramírez-Orejel

et al. 2019

Food Science & Nutrition

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Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from a single Bovans White flock and were exposed to L. monocytogenes . Artificially contaminated eggs were divided into three different treatment groups: NEW, 2% citric acid solution (CAS), and saline solution (SS). To evaluate the bactericidal effect, the Mexican norm for antimicrobial activity determination protocol was performed. The observed bactericidal effect was compared against those obtained from CAS and SS. Bacterial cells present on the eggshells were quantified. NEW exhibited a significantly higher bactericidal effect than CAS when evaluated on the surfaces of chicken eggshells (6.11 log 10 CFU/ml reduction in vitro and a 2.18 log 10 CFU/egg reduction on eggs vs. 1.06 log 10 CFU/ml in vitro reduction and 1.74 log 10 CFU/egg). Additionally, CAS was found to react with the carbonate egg shield, resulting in a loss of cuticle integrity. Mineral content of NEW‐treated eggshells was similar to SS‐treated eggshells; however, CAS‐treated eggshells showed a significant decrease in phosphorous concentration compared to NEW treatment. In this study, we demonstrated the effect of NEW and CAS on the integrity of the L. monocytogenes wall using transmission electron microscopy. To the best of our knowledge, this is the first report of the effect of NEW against L. monocytogenes on eggshells. Our results show that NEW is a viable alternative solution for the disinfection of table eggs that does not affect the cuticle or shell.

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“…Several studies on different parts of the world, including Bangladesh (Haque et al, 2021), India (Arathy et al, 2009), Egypt (Mansour et al, 2015), Nigeria (Adabara et al, 2020), and South Africa (Jambalang et al, 2017) have revealed the presence of E. coli in table eggs. The same prevalence of E. coli in egg shells found in this study was previously reported in farm eggs in Nigeria (9.1%, Adabara et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the egg shells, the prevalence of E. coli was detected in Zambia (Kapena et al, 2020), and Thailand (Siriporn et al, 2015). Besides, E. coli was isolated from egg contents in several previous studies conducted in Trinidad, Egypt, and Nigeria (Adesiyun et al, 2005;Mansour et al, 2015;Adabara et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance of Escherichia coli and Salmonella Species Isolated from Table Eggs in Morocco

Ftouhy¹,

Hmyene²,

Nacer³

et al. 2023

WVJ

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The development of antimicrobial resistance has become a severe global public health emergency. Foods of animal origin are considered possible drivers of resistant bacteria, including Escherichia coli (E. coli) and Salmonella spp. It is associated with the indiscriminate use of antibiotics, resulting in the inability to treat patients infected with antibiotic-resistant pathogens and a high risk of transmission of these resistant pathogens. The current study aimed to determine the prevalence and antibiotic resistance of E. coli and Salmonella spp. in raw table eggs in Morocco. A total of 870 table eggs resulting from 290 samples (3 eggs = 1 sample), were purchased from ambulatory sellers, street vendors, kiosks, and neighborhood markets from different cities in Morocco and transferred to the laboratory in the Hassan II Agronomy and Veterinary Medicine Institute of Rabat, Morocco. The egg shells and contents were tested separately then the isolation and identification of bacterial pathogens were performed according to the Moroccan Standard Norms. The bacterial isolates were tested for susceptibility to six commonly used antibiotics, namely nalidixic acid (30 µg), kanamycin (30 µg), gentamycin (15 µg), ciprofloxacin (15 µg), tetracycline (30 µg), and amoxicillin (10 μg). The findings revealed that 38 samples (13%) tested positive for E. coli of which 9% were on egg shells, and 4% were in egg content, while for Salmonella enteritidis (S. enteritidis), 5 samples (2%) tested positive and only in the egg contents. Escherichia coli showed the highest resistance to amoxicillin, followed by tetracycline and nalidixic acid with 92.10%, 84.21%, and 50%, respectively, and was sensitive to ciprofloxacin (84.21%), kanamycin (65.79%), and gentamicin (60.54%). Salmonella enteritidis had the highest resistance against tetracycline (80%), followed by ciprofloxacin and nalidixic acid with 40% each. The highest sensitivity rates of S. enteritidis were for gentamicin, amoxicillin, and kanamycin at 80%, 80%, and 40%, respectively. Given that these resistant bacteria could potentially be transferred to humans through eggs or egg products, it is necessary to employ strict hygiene measures and provide a wise and legal use of antibiotics in animal breeding.

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Contamination of the Shell and Internal Content of Table Eggs With Some Pathogens During Different Storage Periods

Cited by 13 publications

References 26 publications

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes

Antibiotic Resistance of Escherichia coli and Salmonella Species Isolated from Table Eggs in Morocco

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