Construction of simultaneous SPR and QCM sensing platform

Abstract

To construct a novel simultaneous SPR and QCM sensing system, an AT-cut quartz crystal is fabricated by sputtering 250 nm of ITO on one side of the quartz plate over a 5-nm thick underlay of titanium, while a 50-nm thick layer of gold is sputter-deposited on the other side to induce a total light reflection of an incident laser beam on the thin gold layer. The signals of the sensing system are detected using a Handy-SPR and QCA922 when dropping 200 microL of Milli-Q water into the sensing cell. A decrease in the SPR reflected light intensity is clearly identified. In the same experiment, the changes in the resonant frequency and resistance are about 2 kHz and 200 Omega, respectively. Furthermore, the oscillation stabilities of the resonant frequency and resistance are about 50 Hz and 2 Omega, respectively, which are sufficient to detect a large mass change on the QCM/SPR chip.

The principles and applications of nano-diagnosis system for a nano-biosensor

“…Studies on the change in viscoelastic properties in the mirco-area by integrating QCR and atomic force microscopy, on the change in the permeability of the PPy films based on oxidation-reduction reactions by integrating QCR and UV-visible spectroscopy, and on the analysis of the morphological structure of thin films based on the viscoelastic change using QCR and AFM were reported. A system simultaneously analyzing QCR and surface plasmon resonance (SPR) responses is actively being researched to improve the reliability and sensitivity of the sensor system [73]. In the current review, the principles of the measurement, the concrete examples of nano-sensing using the quartz crystal and the possible applications in biological science will be briefly described.…”

“…Thus, the quartz crystal microbalance was further developed as quartz crystal analyzer (QCA). Currently, it known as a measuring device for fine mass and the viscoelastic change, and various applications using its properties have been reported in the areas of immune assay, the observation of gelation reactions, electrochemical experiments, the observation of the changes in the rheological properties of organic thin films, and viscoelastic measurements in the nano-meter area [8,[62][63][64][65][66][67][68][69][70][71][72][73].…”

“…To analyze viscoelasticity of polymer films, the quartz crystal resonator (QCR) and differential scanning calorimetry (DSC) were simultaneously used by the authors' group [69,70]. In addition, morphological changes were also observed using AFM [71][72][73][74]. Studies on the change in viscoelastic properties in the mirco-area by integrating QCR and atomic force microscopy, on the change in the permeability of the PPy films based on oxidation-reduction reactions by integrating QCR and UV-visible spectroscopy, and on the analysis of the morphological structure of thin films based on the viscoelastic change using QCR and AFM were reported.…”

New trends in instrumental design for surface plasmon resonance-based biosensors

“…Moreover, it could be combined to additional devices such as electrochemical instrumentation (Baba et al 2006). Other recent combinations include surface acoustic wave sensor (Bender et al 2009; Francis et al 2006; Galopin et al 2007), HPLC (Du and Zhou 2008), and quartz crystal microbalance (QCM) (Kim et al 2010). …”

Collagen immunoassay as a method to optimise surface functionalisation

“…It is very effective at determining changes in mass caused by the attachment of molecules, including proteins, to functionalised surfaces . While enhancements to QCM, such as simultaneous SPR, QCM with dissipation (QCM‐D), and QCM with electrochemistry (EQCM) provide insights into chemical bonds and viscoelastic properties of conjugated layers, significant conjecture about bond types and conjugation strength is required.…”