Construction of a Novel Biosensor Based on the Self-assembly of Dual-Enzyme Cascade Amplification-Induced Copper Nanoparticles for Ultrasensitive Detection of MicroRNA153

Cui, Jingyu; Han, Houyu; Piao, Jiafang; Shi, Hai; Zhou, Dianming; Gong, Xiaoqun; Chang, Jin

doi:10.1021/acsami.0c06032

Cited by 25 publications

(12 citation statements)

References 36 publications

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“…The characteristic of copper nanoclusters (CuNCs) to produce red fluorescence under ultraviolet (UV) radiation has attracted wide attention and applications of scientists in biosensor design [ 11 , 12 ]. Ligands such as nucleic acids [ 13 ], cysteine [ 14 , 15 ], and bovine serum albumin [ 16 , 17 ] can generate CuNCs in the presence of reducing agents.…”

Section: Introductionmentioning

confidence: 99%

Visualization of copper nanoclusters for SARS-CoV-2 Delta variant detection based on rational primers design

Zhu

2022

Talanta

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Here, based on the design of rational primers and copper nanoclusters (CuNCs), we present a method for the accurate detection of the SARS-CoV-2 Delta variant, which is capable of distinguishing the Delta variant with its single nucleotide polymorphism from the ‘wild type’ coronavirus (NC_045512.2), and realizing visualization signal out. Speciﬁcally, we show that dual priming oligonucleotide (DPO) primers and AT primers can be used to distinguish between wild types and mutations of this virus by polymerase chain reaction (PCR) analysis and that visualization can be achieved via the red fluorescence of CuNCs in ultraviolet radiation. Among the results, it was found that the R-1-down (DPO)-6I and F-1-30AT, with the single nucleotide deletion site designed at the 3′ end of the downstream primer, showed the best specificity towards the Delta variant. Moreover, the use of AT primers increased the AT contents of the PCR products, thus meeting the template requirements generated by the CuNCs. It was also found that the AT primers could assist with improving detection specificity. Finally, we demonstrate that the visualization of the CuNCs-based detection assay exhibited a linear relationship in 0.5 pg μL −1 –50 ng μL −1 , with a limit of quantitation (LOQ) of 0.5 pg μl −1 .

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Section: Introductionmentioning

confidence: 99%

Visualization of copper nanoclusters for SARS-CoV-2 Delta variant detection based on rational primers design

Zhu

2022

Talanta

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“…Nanotechnology, with the improvement and application of the nano‐particles (NPs)/nanocarriers with dimension in the nanoscale range (1 ~ 100 nm) (Gong et al, 2018; Piao et al, 2019; B. Zhang, Gao, et al, 2018), has been widely utilized in a variety of fields, including medicine (Emerich & Thanos, 2003), pharmacy (Li et al, 2020), biological detection (Chen et al, 2020; J. Cui et al, 2020; Peng, Huang, et al, 2020), optics (Ochmann et al, 2017; Peng, Qin, et al, 2020), and agriculture (Curtis & Wilkinson, 2001; Prasad et al, 2017). The extremely small size of NPs allows efficient entrance into living system such as animal and human body.…”

Section: Introductionmentioning

confidence: 99%

Applications of nanotechnology in virus detection, tracking, and infection mechanisms

Kang

Tahir

Wang

et al. 2021

WIREs Nanomed Nanobiotechnol

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Viruses are among the most infectious pathogens, responsible for the highest death toll around the world. Lack of effective clinical drug for most of the viruses emphasizes the rapid and accurate diagnosis at early stages of infection to prevent rapid spread of the pathogens. Nanotechnology is an emerging field with applications in various domains, where nano‐biomedical science has many significant contributions such as effective delivery of drugs/therapeutic molecules to specific organs, imaging, sensitive detection of virus, and their accurate tracking in host cells. The nanomaterials reported for virus detection and tracking mainly include magnetic and gold NPs, ZnO/Pt‐Pd, graphene, and quantum dots (QDs). In addition, the single virus tracking technology (SVT) allowed to track the life cycle stages of an individual virus for better understanding of their dynamics within the living cells. Inorganic as well as non‐metallic fluorescent materials share the advantages of high photochemical stability, a wide range of light absorption curves and polychromatic emission. Hence, are considered as potential fluorescent nano‐probes for SVT. However, there are still some challenges: (i) clinical false positive rate of some detection methods is still high; (ii) in the virus tracking process, less adaptability of QDs owing to larger size, flicker, and possible interference with virus function; and (iii) in vivo tracking of a single virus, in real time needs further refinement. In the future, smaller, non‐toxic, and chemically stable nanomaterials are needed to improve the efficiency and accuracy of detection, and monitoring of virus infections to curb the mortalities. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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“…T junctions (also called three-way junctions) that formed of three strands of DNA rely on the Watson–Crick complementary base pairing principle and are a type of stable architecture with demonstrated versatility in the biosensing field. , Cheng et al reported a kind of defective T junction that contained an artificial vacuole at the junction site and was more conformationally stable than the traditional T junction . To overcome the shortcomings of the normal SDA strategy, herein, through the ingenious design of a pair of dual-function probes, a new SDA method in which the defective T junction structure is first used to support SDA (dT-SDA) is proposed.…”

Section: Introductionmentioning

confidence: 99%

Study on Defective T Junction-Mediated Strand Displacement Amplification and Its Application in Microchip Electrophoretic Detection of Longer Bacterial 16S rDNA

et al. 2021

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Current strand displacement amplification (SDA)-based nucleic acid sensing methods generally rely on a ssDNA template that involves complementary bases to the endonuclease recognition sequence, which has the limitation of detecting only short nucleic acids. Herein, a new SDA method in which the defective T junction structure is first used to support SDA (dT-SDA) was proposed and applied in longer DNA detection. In dT-SDA, an auxiliary probe and a primer were designed to specifically identify the target gene, following the formation of a stable defective T junction structure through proximity hybridization, and the formation of defective T junctions could further trigger cascade SDA cycling to produce numerous ssDNA products. The quantity of these ssDNA products was detected through microchip electrophoresis (MCE) and could be transformed to the concentration of the target gene. Moreover, the applicability of this developed strategy in detecting long genomic DNA was verified by detecting bacterial 16S rDNA. This proposed dT-SDA strategy consumes less time and has satisfactory sensitivity, which has great potential for effective bacterial screening and infection diagnosis.

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Construction of a Novel Biosensor Based on the Self-assembly of Dual-Enzyme Cascade Amplification-Induced Copper Nanoparticles for Ultrasensitive Detection of MicroRNA153

Cited by 25 publications

References 36 publications

Visualization of copper nanoclusters for SARS-CoV-2 Delta variant detection based on rational primers design

Visualization of copper nanoclusters for SARS-CoV-2 Delta variant detection based on rational primers design

Applications of nanotechnology in virus detection, tracking, and infection mechanisms

Study on Defective T Junction-Mediated Strand Displacement Amplification and Its Application in Microchip Electrophoretic Detection of Longer Bacterial 16S rDNA

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