MicroRNAs
(miRNAs) have received extensive attention because of their potential
as biomarkers for cancer diagnosis and monitoring, and their effective
detection is very significant. Here, a specific, one-pot, rapid, femtomolar
sensitive miRNAs detection biosensor was developed based on the target-triggered
three-way junction (3-WJ) and terminal deoxynucleotide transferase
(TDT)/Nt.BspQI in combination with activated copper nanoparticles
(CuNPs) self-assembly. To this end, a 3-WJ hairpin probe and helper
probe were designed to selectively identify the target miRNA, so as
to form a stable 3-WJ structure that further triggered the double-enzyme
cycling to produce poly T to activate the self-assembly of CuNPs.
Based on the simplicity of CuNPs generation, the poly T template fluorescence
CuNPs can detect the minimum detection limit of 1 fm within 1.75 h.
In addition, the applicability of this method in complex samples was
demonstrated by analyzing the whole-blood RNA extraction from Parkinson
patients, consisting of the results of commercial miRNA kits. The
developed strategy performs powerful implications for miRNA detection,
which may be beneficial for the effective diagnostic assays and biological
research of Parkinson’s disease.
Nanotechnology‐basedin vitro diagnostics (nanoIVDs) are widely studied for disease diagnostics with promising sensitivity, specificity, and convenience. However, it is still a major challenge for both regulatory authorities and scientific researchers to accelerate the clinical translation of such an innovative technology. Herein, this perspective discussed the benefits and challenges of nanoIVDs as well as the administration considerations on the distinguish features of nanoIVDs by regulatory authorities, so as to further achieve the evaluation, translation, and application of nanoIVD products.
Chromogenic detection of target objects has attracted extensive attention in the academic field with high specificity and sensitivity. Based on color changes in chemical reactions, biomolecular analysis with simple and rapid signal readout in disease diagnosis can be realized. At present, three main mechanisms of chromogenic detection were proposed, including proteases catalysis, enzyme mimics catalysis, and chemical reactions based on inorganic or organic for biomolecular analysis. With the unremitting efforts of scientific researchers, the evolution of chromogenic detection has been developed from the early qualitative detection to the current quantitative detection, accompanied by the improvement of sensitivity. Herein, this paper reviewed the mechanisms and relevant applications of chromogenic detection from the above three aspects, discussed the challenge, and provided a future perspective in this emerging field.
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