Nanoshells have been previously shown to tune absorption
frequencies
efficiently. What will happen when a nanoshell embeds on a small core
system? A theoretical model that a core composed of gold is embedded
within a nanoshell of Au/Ag is constructed to answer this question.
The calculations based on the discrete dipole approximation (DDA)
method are performed and proved to converge accurately by satisfying
the usual criteria related to the applicability of the DDA. The results
show that the nanoshells in the core/shell system greatly influence
the surface plasmon resonance (SPR). Indeed, the shell frequency is
tuned to match the optical properties of the absorbing core leading
to hybridization/mixing and possibly overall enhancement of absorption
cross-section. The calculation of the field enhancement also shows
that the location of the field enhancement is specified by the different
resonance patterns.
Current strand displacement amplification
(SDA)-based nucleic acid
sensing methods generally rely on a ssDNA template that involves complementary
bases to the endonuclease recognition sequence, which has the limitation
of detecting only short nucleic acids. Herein, a new SDA method in
which the defective T junction structure is first used to support
SDA (dT-SDA) was proposed and applied in longer DNA detection. In
dT-SDA, an auxiliary probe and a primer were designed to specifically
identify the target gene, following the formation of a stable defective
T junction structure through proximity hybridization, and the formation
of defective T junctions could further trigger cascade SDA cycling
to produce numerous ssDNA products. The quantity of these ssDNA products
was detected through microchip electrophoresis (MCE) and could be
transformed to the concentration of the target gene. Moreover, the
applicability of this developed strategy in detecting long genomic
DNA was verified by detecting bacterial 16S rDNA. This proposed dT-SDA
strategy consumes less time and has satisfactory sensitivity, which
has great potential for effective bacterial screening and infection
diagnosis.
Nanozymes have enzyme-like characteristics and nanozyme-based electrochemical sensors have been widely studied for biomarker detection. In this work, cuprous oxide-modified reduced graphene oxide (Cu2O-rGO) nanozyme was prepared by simultaneous reduction...
The sensitive detection of biomarkers
is crucial for the early
identification and treatment of cancer. In this study, a cysteine–histidine–Cu-modified
jujube-like Cu2O (CH-Cu@J-Cu2O) nanozyme was
synthesized and used to fabricate an electrochemical sensor for mucin-1
(MUC1) sensitive detection. Gold-modified reduced graphene oxide and
CH-Cu@J-Cu2O for the sensor were prepared and also characterized
by transmission electron microscopy (TEM), scanning electron microscopy
(SEM), high-resolution transmission electron microscopy (HRTEM), X-ray
diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The
CH-Cu@J-Cu2O nanozyme was used as a signal probe, containing
two catalytic units of cysteine–histidine–Cu and jujube-like
Cu2O. The CH-Cu@J-Cu2O nanozyme can potently
catalyze the H2O2-driven oxidation of dopamine
to aminochrome, leading to a high-level electrochemical signal. This
electrochemical sensor was used to detect MUC1 with a linear range
from 0.5 to 5000 pg·mL–1, and the limit of
detection was 0.085 pg·mL–1, owing to the excellent
catalytic activity of CH-Cu@J-Cu2O. The expression of MUC1
on the surface of MCF-7 cells was further analyzed, and the results
indicate that the proposed strategy is practical for the detection
of biomarkers.
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