Construction of a multi-layer skin substitute: Simultaneous cultivation of keratinocytes and preadipocytes on a dermal template

Keck, Maike; Haluza, Daniela; Lumenta, David B.; Burjak, S.; Eisenbock, B.; Kamolz, Lars‐Peter; Frey, Manfred

doi:10.1016/j.burns.2010.07.016

Cited by 45 publications

(35 citation statements)

References 27 publications

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“…2 and in the previous study. 32 Similar performance was achieved with 7 days of coculture in a previous study. 33 Another study showed that a period of 7 days is optimal for coculture between fibroblasts and epithelial cells.…”

Section: Discussionsupporting

confidence: 68%

A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat

Hilmi

Hassan

Halim

2015

Advances in Wound Care

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Objective: An engineered skin substitute is produced to accelerate wound healing by increasing the mechanical strength of the skin wound via high production of collagen bundles. During the remodeling stage of wound healing, collagen deposition is the most important event. The collagen deposition process may be altered by nutritional deficiency, diabetes mellitus, microbial infection, or radiation exposure, leading to impaired healing. This study describes the fabrication of an engineered bilayer skin substitute and evaluates its effectiveness for the production of collagen bundles in an impaired healing model. Approach: Rats were exposed to 10 Gy of radiation. Two months postirradiation, the wounds were excised and treated with one of three skin replacement products: bilayer engineered skin substitutes, chitosan skin templates, or duoderm ª . The collagen deposition was analyzed by hematoxylin and eosin staining. Results: On day 21 postwound, the irradiated wounds displayed increased collagen bundle deposition after treatment using bilayer engineered skin substitutes (3.4 -0.25) and chitosan skin templates (3.2 -0.58) compared with duoderm (2.0 -0.63). Innovation: We provide the first report on the fabrication of bilayer engineered skin substitutes using high density human dermal fibroblasts cocultured with HFSCs on chitosan skin templates. Conclusion: The high density of fibroblasts significantly increases the penetration of cells into chitosan skin templates, contributing to the fabrication of bilayer engineered skin substitute.

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Section: Discussionsupporting

confidence: 68%

A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat

Hilmi

Hassan

Halim

2015

Advances in Wound Care

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show abstract

“…Commercially available collagen-based dermal substitutes such as Matriderm ® are commonly used in skin engineering. Using this scaffold, a full-thickness skin graft with human ASC and keratinocytes was engineered, which demonstrated that ASC were able to migrate into the dermal substitute, similar to dermal fi broblasts [ 59 ]. Another investigation using collagen as a dermal substitute with human ASC, dermal fi broblasts and keratinocytes demonstrated that using ASC produced full-thickness skin with a stratifi ed epidermis of equal quality to dermal fi broblasts [ 60 ].…”

Section: Wound Healing and Skin Engineeringmentioning

confidence: 97%

Human Adipose-Derived Stem Cells (ASC): Their Efficacy in Clinical Applications

Locke

Feisst

2014

Regenerative Medicine

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“…For example, a skin substitute model comprised of keratinocytes and preadiposites was designed for preparation of a multilayer skin substitute that may function in replacement of epidermal, dermal and hypodermal skin layers [99]. This might prove beneficial in patients with deep fullthickness burn injuries.…”

Section: Increased Complexity Of Skin Substitutesmentioning

confidence: 99%

Skin substitutes for burn wound healing: current and future approaches

Supp¹

2011

Expert Review of Dermatology

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Construction of a multi-layer skin substitute: Simultaneous cultivation of keratinocytes and preadipocytes on a dermal template

Cited by 45 publications

References 27 publications

A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat

A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat

Human Adipose-Derived Stem Cells (ASC): Their Efficacy in Clinical Applications

Skin substitutes for burn wound healing: current and future approaches

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